LOCUS       sequence-191919-        2903 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PFBA1-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pFBA1-1
FEATURES             Location/Qualifiers
     source          1..2903
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1199..1215)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1199..1215)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1263..1294
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1295..1397
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1398..2213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1471..1490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2237..2264
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2276..2863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2356..2375)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2903,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggaggaa tgaagttatt ggtcttgggt gcagctttcc
      181 atctgttcga tttattcggc taagagttta ccattgtgtg ctcgtatggg gaagggtgca
      241 aggatcagta atacagtcga acctggagta tctaccatag tggggataca atgtagttta
      301 tctgttatct cgattgttcc taattaaggt tttctttgat cctcttctag tccacacctc
      361 ctagatgaca ttcgagctgc ctggattgga tgcctaggtt tattgcctag ttcaatacaa
      421 ttcgtgcggg ctacagtaga aggcccttac ataatccgga aagcatggtc ccccaccaaa
      481 ttgagagctt tttcagcctt cactggtggt atcattttcg ggagataata aggtttcgat
      541 tgggaattcc caccagagaa cactatagag ggaccaagct gatgctagcc tgacatcccc
      601 aaagcacact tcgtaattga aaaccgttac ctctagcaca ctgtccagac tacccccgtc
      661 aaaaaaacgc tctttttctc gactaattga gtctgcaact catcccgtcc ttgccgaatt
      721 acttgaattc atttcacacc tccgttgctt acgtactctc accggcctcc ggtgtacatg
      781 gatccgctat tgccagatat ttctcataca acaatcacca gatcaaggtc gtgaacggac
      841 caatggcatc cagagcaatc ctgaacagat aggggtccgg gctgtataaa gtgaaataac
      901 gtgacttgaa ccagcaacta tgtcccagtt gtgctacact taacacgcga ttaccccgga
      961 gctcaccagg cctcttcccc ctctcattgg aaccctccta gcgcttcgaa ataatggctg
     1021 cgtactattt aactggtgcc agttcccgct gacaatatcc tttttcttct ccccttagtt
     1081 ccccacatat caattgaaca tattttttac accatggcgt cttcgaattc gcgcgtgatc
     1141 ttacggcatt atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt
     1201 catagctgtt tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag
     1261 aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact
     1321 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1381 gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgcttg aagccgcgat
     1441 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1501 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     1561 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc
     1621 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1681 ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg
     1741 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1801 ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1861 gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg
     1921 ttgaacaagt ctggaaagaa atgcataaac tcttgccatt ctcaccggat tcagtcgtca
     1981 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     2041 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     2101 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     2161 atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc taatgaggac
     2221 ctaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc
     2281 ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa
     2341 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2401 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2461 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2521 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2581 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2641 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2701 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg
     2761 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2821 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt
     2881 ctaccgaaga aaggcccacc cgt
//
