LOCUS       sequence-19192-e         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf109
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             526..567
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             577..594
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(617..634)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    623..734
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gatccagtac ccttcaccga attccagctg accaccatga taccagtagt ttactgtata
       61 cactcaaatg ccgaatatgg ttataacaat attactaaca taaagataat acgtaaacta
      121 cttaatctta gtagacgtgc gtcacataat ctatttagag atcgagtcat gcacgattat
      181 ataagtaata catatattga tcttgagtgt ttagatatta ttagatcgtt ggatggattc
      241 gatatcaatg gttactttga aggacgtaca ccacttcatt gcgctataca acataacttc
      301 actcagattg ctaagtactt attagatcga ggagctgata tagtcgtacc caacacattg
      361 attatacatc agtacagaca gtaagcaatt cccggggatc cgcattgatt atacatcagt
      421 acatacagta agcaattccc ggggatccga ataaagggtc aagacaattc tgcagatatc
      481 cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa gcctatccct
      541 aaccctctcc tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt
      601 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
      661 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
      721 gaggaaattg catcgcattg tctgagtag
//
