LOCUS       sequence-191929-        2928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PSHB17.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pSHB17
FEATURES             Location/Qualifiers
     source          1..2928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1224..1240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1224..1240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1288..1319
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1320..1422
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1423..2238
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1496..1515)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2262..2289
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2301..2888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2381..2400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2928,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggaggca aggcaactga gaaattgaat agtggtttca
      181 agcccgctga ctttttgtat tatctcaatg tcggtgtttc acagtcccca gaagggggct
      241 ttgccttcaa gggagacgga agagacatcg tcaaccctgg ggagaagtat ttcaaatggc
      301 gcaagttcgc taatttttac gattaagcag tgctgtatgg ggtagttaat aaatcgggaa
      361 tatccttctg acgtgactgt aacaaatctc tttttacgtg gtgcgcatac tggacagagg
      421 cagagtctca atttcttctt ttgagacagg ctactacagc ctgtgattcc tcttggtact
      481 tggatttgct tttatctggc tccgttggga actgtgcctg ggttttgaag tatcttgtgg
      541 atgtgtttct aacacttttt caatcttctt ggagtgagaa tgcaggactt tgaacatcgt
      601 ctagctcgtt ggtaggtgaa ccgttttacc ttgcatgtgg ttaggagttt tctggagtaa
      661 ccaagaccgt cttatcatcg ccgtaaaatc gctcttactg tcgctaataa tcccgctgga
      721 agagaagttc gaacagaagt agcacgcaaa gctcttgtca aatgagaatt gttaatcgtt
      781 tgacaggtca cactcgtggg ctatgtacga tcaacttgcc ggctgttgct ggagagatga
      841 caccagttgt ggcatggcca attggtattc agccgtacca ctgtatggaa aatgagatta
      901 tcttgttctt gatctagttt cttgccattt tagagttgcc acattcgtag gtttcagtac
      961 caataatggt aacttccaaa cttccaacgc agataccaga gatctgccga tccttcccca
     1021 acaataggag cttactacgc catacatata gcctatctat tttcactttc gcgtgggtgc
     1081 ttctatataa acggttcccc atcttccgtt tcatactact tgaattttaa gcactaacat
     1141 ggcgtcttcg aattcgcgcg tgatcttacg gcattatacg tatgatcggt ccacgatcag
     1201 ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata ctgaccattt
     1261 aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt
     1321 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta
     1381 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg
     1441 gaaacgtctt gcttgaagcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     1501 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     1561 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     1621 gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     1681 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca
     1741 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     1801 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg cgatcgcgta
     1861 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt
     1921 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaactcttg
     1981 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2041 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2101 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2161 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg
     2221 ctcgatgagt ttttctaatg aggacctaaa tgtaatcacc tggctcacct tcgggtgggc
     2281 ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2341 cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2701 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2761 gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2881 gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgt
//
