LOCUS       sequence-19193-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf110
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             548..589
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             599..616
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(639..656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    645..756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaaccaccat ggaggaggat
       61 acaaatattt caaataaagt tataaggtac aacactgtca ataatatatg ggaaacatta
      121 cctaacttct ggactggaac tataaatcca ggcgtggtct cgcataaaga tgatatatat
      181 gttgtatgcg acatcaaaga tgaaaaaaat gttaaaactt gtatatttag atataacacg
      241 aatacgtata acggatggga attggtcacg acgacagaaa gcagattatc agctctgcat
      301 actattcttt ataacaatac cataatgatg ttacattgtt atgaatcgta tatgttacaa
      361 gatacattta atgtgtacac tcgcgaatgg aatcatatgt gtcatcaaca ttcgaatagt
      421 tatatcatgt acaatatact acccatctac taagcaattc ccggggatcc gaataaaggg
      481 tcaagacaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgcg
      541 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
      601 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
      661 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
      721 cactgtcctt tcctaaataa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
      781 tatctggggg ggggg
//
