LOCUS       sequence-191934-        2939 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PLAT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pLAT1
FEATURES             Location/Qualifiers
     source          1..2939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1235..1251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1235..1251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1299..1330
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1331..1433
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1434..2249
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1507..1526)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2273..2300
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2312..2899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2392..2411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2939,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggaggga agaatatgaa ccacgattga aagactttgg
      181 gtttgccgat tctaaaacac ttactgaaca gaaaagaagt gaattattag agagtttatg
      241 caacggagat catgagctta gaaagaacgt aggctgggca acaacatcca tttctgcctg
      301 cgatatttcc agtgcaatgc taaagccaca taatataggc aattacaact tgaatgagca
      361 ggctcatgat gtcaccatgg aactgataga taaagtcatc caaaaaggtg tcaatttacg
      421 gcacgtctac gtagatactg tcggacctcc agcatcatac caaaagaaac tgcaacaaag
      481 gtttcccgca ctgctgatca ccgtatctaa aaaagcagat gccatctatc catgtgtttc
      541 aagtgcttca gtttgcgcca aggtcactag agacgtctgt ttgagaacct caaatgggga
      601 ggagaaccta ttagattgtg ggtctggata tccctcggac ccaaatacta agaaatggct
      661 agcccagtct atacatccat tgtttggctg gagtcgtgat gtcaggtttt cgtggcagac
      721 ggccaaagat tgtcttgaga gacacaactc aatcaaagtt gtgtttggcg aagaggagcc
      781 taccccggtt aatttcggtg atctggggtt gagcaaacag aagcccgact ttaaggattt
      841 agaaatcagt accagttggt ggggacatta attggtggag gagagccaat attctcaact
      901 gttcgtagga ccataacccc ccactcgcac cagaaatact cattggcgct gacaactagt
      961 ttgagccgct tcaattgcgg caagtcccca gttttgtttg gtactcccct ctcgcctcgc
     1021 gtgtttttgt tcctccgaat tgtgcatcac cctgtcacat ccgtacacca tcggaacact
     1081 ctgctcctcc tcctttctcc tcttaattga acgtatatct aattacccta ataacaagta
     1141 tcttcaacca tggcgtcttc gaattcgcgc gtgatcttac ggcattatac gtatgatcgg
     1201 tccacgatca gctagattat ctagtcagct tgatgtcata gctgtttcct gaggctcaat
     1261 actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag
     1321 gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1381 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagcc
     1441 atattcaacg ggaaacgtct tgcttgaagc cgcgattaaa ttccaacatg gatgctgatt
     1501 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     1561 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1621 atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg cctcttccga
     1681 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatcccag
     1741 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1801 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaacg
     1861 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttggtg
     1921 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1981 ataaactctt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     2041 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     2101 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2161 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2221 ttcacttgat gctcgatgag tttttctaat gaggacctaa atgtaatcac ctggctcacc
     2281 ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2341 atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2401 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2461 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2521 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2581 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2641 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2701 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2761 ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc
     2821 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2881 cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgt
//
