LOCUS       sequence-191938-        5247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle Sgd1 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Sgd1
FEATURES             Location/Qualifiers
     source          1..5247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2836..2935)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2952..2971)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2953..2971)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2976..2992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2976..2992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3094..3113)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3105..3914
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3172..3191)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4061..4649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4550..4569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4803..4820
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4979..5006)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5098..5184)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(5233..5247,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(5247,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg caaaaaacag atggtattag aattccaggc gttattttgg
      181 acgagttgaa gactcttgac tacagtcaag atgaaaggtt ttctatttca gaaggaaaaa
      241 agagaaaacg aggaaatggc aaacatttaa gccgtaaaga aaagagaaag atggaacggg
      301 ccgacaagaa acgtaaaatt atatcaacta gagaaatcaa ttccagccgg ctaaaatcag
      361 caccaacgtc cgaaaagagg agtgctaatg caggtgtgaa aaacgtaggc aagcaggcta
      421 acggtaaaaa tccgatttct tcagatgaat cagaaagtaa tgaaaactgg gatagtgacg
      481 aagtgcttac tgatgaagta gcagaagaaa gcggtgagca ggcaatgtct gcggaagaaa
      541 caatgaaaaa actagaatca ttgaaacgga aagcaaaggg tatacaagga gctgaaaact
      601 cgggagaaat aaaaggtaac tcatatgaaa agaaacatat tcgtaacaga gatacaaatg
      661 agaattttgt gagttatccg ctggcccctt cagacagatc agcgtttgaa agggacgaga
      721 tggacatgca atattacgca aaaaagttgg gcttgaaagg agaaaggaag gctatacatg
      781 caaaagatga atttgatgct attggtggac ttcttgaagg tttggaatac tttgaaaatt
      841 atggcaaaag tgacgaagag tatggagact ttgccacaga aactaattct atgagaaaag
      901 atgatgaagc aagcgagaaa gcattttcct cggatgatga tctttcagct tctgattttg
      961 aagactcaga tgggcttagt gaatctgata acgacagcgt tgcggactcg gatgataatt
     1021 atagaagaga aaaggaaaat ccctatgtag ctcccgcaca atctgtagag agctatgttc
     1081 ccccatcact gagaaagaaa ttggatgata gtgaaaacaa tagtactttg tttgaaatta
     1141 gcaagaaggt caactcctct ttgaataagc tttcagactc caatattact attattatca
     1201 ctgatttaaa cagattgtat gattccctgc ctaggcagta cgttacagaa tcgttaacga
     1261 aagggatatt gaatatcata agccaaaatc agaagttgtt ggacggattt ataatgaatt
     1321 atgctgctct tgcgtataca ttatcaaaac taagaggtat tgaggtgggt gcttttttca
     1381 ttcaaaaaac ggtagaggca tttctgcatc attacgaaga ggaaatggaa aatatattaa
     1441 aggatcagca gagcaaaatt tcttctaaaa tatgcatcaa tattgcaacc ttactatcgt
     1501 attgttataa ttttggattt gtttcgtgtc gcttaatcta tgacattatt cgaatatttg
     1561 tagcagatcc taatgaattc actactgagt tacttttacg aattatatcg atttcaggac
     1621 aactgatccg gggtgatgat ccatccgccc tgagagatat acgatcagaa ctgttgaaga
     1681 acgccaagaa tttaaaagag cagagcccaa gattgagatt tttaatggat accatgtctg
     1741 acttgaaaaa caacaggtta aagccttcta ttttggcgac tgatcatcat ccattaaaga
     1801 aaaacttgca aagcattttg aattcttctt cttcttggga gcctttacag gtttctctgg
     1861 acgacattaa aaacattgat tccaagggga aatggtggct ggttggtgct tcttggcgag
     1921 gaaacatgga aaatgctttt gaagtttcga tcaataatga aaatgatgcc agcaagtcca
     1981 agaaatctaa aatttcaatc gaggatgatc ttcttgatga tattccagat tggaatatta
     2041 tcgccagaca acagcgtatg aatactgaca ttcgaagggc tatatttata agtattatgt
     2101 ctgcacaaga ttacctggat gcatttagta aacttgaaaa gttaagcttg aaaaacaagc
     2161 aagttcttga aattccaaga attgttttac attgtctttt ggctgatagt ggatccaatg
     2221 gttataatca ttactacgca ttggtcgcga acaaaatatg tgaaaggtat agccatttat
     2281 cgaagtcgtt tcaattccta ttctgggatg tgataaaaaa atttgaagac aaagaattcg
     2341 attctgaaag cgatactgac gaagaagatg atttagatga taaagaaaaa ctgcttcgca
     2401 tctcaaatca ggggagattt tttggatcgt tgcttgctaa tgacattttg aaattagatg
     2461 ttttcaagca tgttccattt atgggtggat taaacacaga agggatgtta tttatggaga
     2521 tattgctatt tcagttattt ttaactgtag ctaaaaaaag tgagaagaaa ctcaagatgg
     2581 atgaaagtgg aaataagcgt ataatctact ccgatgatta tttgcgcgat tttttaacga
     2641 agaacgttaa atcggaaaat atgctattca ttctcaaagg tttgaagtgg tttattaaca
     2701 agaaatttag ataccataat ttccttgcag gaaagaaagg agataaagct tttgataggg
     2761 atgaaagaag attggcttgg gcttcaaaag ctgccaaatc tattatagac aaagaactgg
     2821 aaaatataga ttcgaaccca gctttcttgt acaaagttgg cattataaga aagcattgct
     2881 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     2941 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     3001 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     3061 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     3121 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     3181 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     3241 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     3301 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     3361 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     3421 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     3481 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     3541 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     3601 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     3661 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     3721 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     3781 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     3841 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     3901 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     3961 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     4021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4201 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4741 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4801 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4861 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4921 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa
     4981 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg
     5041 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt
     5101 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc
     5161 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag
     5221 catggatgtt ttcccagtca cgacgtt
//
