LOCUS       sequence-191941-        6100 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle Bms1 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Bms1
FEATURES             Location/Qualifiers
     source          1..6100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3689..3788)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3805..3824)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3806..3824)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3829..3845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3829..3845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3947..3966)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3958..4767
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4025..4044)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4914..5502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5403..5422
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5656..5673
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(5832..5859)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5951..6037)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(6086..6100,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6100,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg gagcagtcta aataaacagc accgtaaggc gaaggagaag
      181 aatacagcaa aaaagaagct ccatacgcag ggtcataatg caaaggcctt tgccgtggca
      241 gccccaggta agatggcgag aactatgcaa aggtcaagtg atgtgaacga aagaaaactg
      301 catgttccta tggttgatcg tacccctgaa gatgatccgc ctccatttat cgtcgctgtc
      361 gtgggtccac ctggaacagg aaagactact ttgattcggt ccctcgtcag gagaatgaca
      421 aaaagcactt tgaatgatat tcaaggtcct attactgttg tctctggtaa acatagaaga
      481 ctaacttttt tggagtgccc tgcggacgat ctgaatgcga tgattgatat tgctaagatt
      541 gccgacttgg ttcttttact aattgacggt aatttcggtt ttgaaatgga aacaatggaa
      601 ttcttaaata ttgctcaaca tcacggtatg ccaagagtac ttggtgtagc tacacatctt
      661 gatctgttta aatctcagtc aactttgaga gcttctaaga aaagattaaa gcacagattc
      721 tggacggaag tttatcaagg tgcaaaatta ttttacctat ctggtgtgat taatggaagg
      781 tatcctgata gagagatttt aaacctttca cgatttatat ctgttatgaa attcagacca
      841 ttaaaatgga ggaacgaaca tccctatatg ttggctgaca gattcacaga tttaactcat
      901 ccggaattaa tagagactca aggactgcaa atcgaccgca aagtcgctat ctatggttat
      961 ttgcacggta ctcctttgcc ttctgctccg ggcactaggg tgcatattgc tggtgttggt
     1021 gatttctcag tagcacaaat tgaaaaatta ccagatccat gtcccacacc tttttaccaa
     1081 cagaaactgg atgattttga acgtgaaaaa atgaaagaag aggcaaaggc taacggagaa
     1141 attacaactg cctctacgac aagaagacgt aaaaggttag atgataaaga taaattgata
     1201 tatgctccaa tgtccgatgt cggaggtgtg ttgatggata aggatgctgt ttatattgat
     1261 attggaaaga aaaatgaaga gccaagtttt gttcccggcc aagaaagagg tgaaggagaa
     1321 aaactcatga cgggtttaca aagtgttgaa caaagtattg cagagaaatt tgatggtgtg
     1381 ggtttgcaat tgtttagcaa cggcactgag cttcatgaag ttgccgatca tgaaggcatg
     1441 gatgttgaaa gtggagaaga aagtattgag gacgatgaag gaaagagcaa gggaagaacc
     1501 agtttaagaa aaccaagaat ttatggtaag ccagttcaag aagaagacgc agatattgat
     1561 aatttaccga gtgatgaaga accttataca aatgatgacg acgtgcagga ctccgaacca
     1621 aggatggttg aaatcgattt caataatacc ggtgagcagg gtgccgaaaa attagctttg
     1681 gaaacagatt ctgaatttga agagagtgaa gatgagtttt cctgggaaag aacagcggca
     1741 aacaagctga agaaaactga aagtaagaaa aggacctgga atatcggtaa gctgatatac
     1801 atggacaaca tttcacctga agaatgtata agaaggtgga ggggcgagga cgacgatagt
     1861 aaagacgaaa gtgacattga agaagatgtt gatgatgact tcttcaggaa aaaagatggt
     1921 actgttacaa aagagggcaa taaagaccat gcagttgatt tggaaaagtt tgttccatat
     1981 ttcgacactt tcgaaaaatt ggctaaaaaa tggaaaagcg ttgatgcaat caaagagcga
     2041 ttcctcggcg caggcatttt aggtaatgat aataaaacga aaagtgactc taatgaaggt
     2101 ggggaagaac tatacggcga ttttgaagat ctagaagatg gaaacccttc tgaacaagca
     2161 gaagacaata gtgacaaaga aagtgaggat gaggatgaaa acgaagacac taatggagat
     2221 gatgataatt cattcactaa ttttgatgcg gaggaaaaaa aggacttaac catggagcaa
     2281 gaaagagaaa tgaatgctgc aaagaaggaa aaactacgtg cacaattcga gatagaagaa
     2341 ggtgaaaact ttaaagaaga tgatgaaaac aatgaatatg atacatggta tgaactacag
     2401 aaggccaaga tatctaaaca gttagaaatt aacaatatag aatatcaaga aatgactcca
     2461 gaacaacgtc aaagaatcga aggtttcaaa gctggttctt atgtacgtat cgttttcgaa
     2521 aaagtcccaa tggaatttgt caagaatttc aatccaaaat ttccaattgt tatgggtggt
     2581 ttattgccga ccgaaataaa gttcggtatt gtcaaagcca ggttgagaag acatcgttgg
     2641 cataagaaga ttttgaagac aaatgaccca ctggtcttgt ctttgggttg gagaaggttc
     2701 caaactttac caatttatac aacaactgac tctagaacaa gaaccagaat gctaaagtat
     2761 acaccagaac acacatattg taatgcggct ttctacggtc ccttatgttc tccaaacaca
     2821 cctttttgtg gtgttcaaat tgttgctaat agtgataccg ggaacggttt tagaattgca
     2881 gcaacaggta tcgttgaaga gatagacgtt aatattgaaa ttgttaaaaa gttaaaatta
     2941 gttgggtttc catataaaat tttcaaaaat actgccttta taaaggacat gttttcaagt
     3001 gctatggaag ttgcaaggtt tgagggtgcg cagatcaaaa ctgtctcggg tatccgtggt
     3061 gaaatcaaaa gggcactttc aaagcctgag ggtcactata gggcagcctt tgaggataaa
     3121 atcctaatga gtgatattgt aattctaaga tcatggtatc ctgtccgcgt caagaaattc
     3181 tataatcctg ttacctcact attgttgaag gaaaaaaccg agtggaaagg tttaagattg
     3241 acaggccaaa taagagcggc tatgaacctg gaaacaccgt cgaatccgga cagtgcatat
     3301 cataaaatag aacgtgtaga aagacacttc aacggtctaa aggtcccaaa agctgttcaa
     3361 aaggaattgc cattcaaatc tcagatccat caaatgaaac cacaaaagaa gaaaacatat
     3421 atggctaaaa gagccgtggt gctaggtggt gatgaaaaga aggcaagatc attcattcaa
     3481 aaagtattga cgatttcgaa agccaaagac agcaagagga aggaacagaa ggctagtcaa
     3541 cgtaaggaaa gattgaaaaa attagctaag atggaggaag aaaaatcaca aagggataag
     3601 gagaaaaaga aagaatactt cgcccagaat ggtaaaagaa caactatggg cggtgacgat
     3661 gaatctcgtc cacgtaagat gaggaggaac ccagctttct tgtacaaagt tggcattata
     3721 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     3781 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     3841 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     3901 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     3961 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     4021 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     4081 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     4141 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     4201 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     4261 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     4321 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     4381 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     4441 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     4501 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     4561 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     4621 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     4681 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     4741 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     4801 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     4861 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4921 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     4981 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5041 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5101 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5161 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5221 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5281 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5341 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5401 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5461 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5521 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5581 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5641 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5701 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5761 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     5821 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     5881 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     5941 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     6001 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     6061 gcgttaacgc tagcatggat gttttcccag tcacgacgtt
//
