LOCUS       sequence-191942-        3238 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PGUT1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pGUT1
FEATURES             Location/Qualifiers
     source          1..3238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1550)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1534..1550)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1598..1629
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1630..1732
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1733..2548
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1806..1825)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2572..2599
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2611..3198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2691..2710)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(3238,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagata aaaaaatatg gcaactttca ccagcttagc
      181 tctccagact ttttcttccc gaatttttgg ttccgagaaa tggacctttg ctagccggtg
      241 ggaaataact tgagatgcat ggacggaatc aaacacggaa aaatctaggt catcctacag
      301 caaacacctg caaggccggg aaagaattgc tcggtatttt ctattttggg agatttgttg
      361 gcacggaaga atcggtaact ttactaatcc aatactccgc tcctgactgt ttcaagtcgg
      421 accccaactt tcaagtgacc caatttagca gcctgcattc tcttgatttt atgggggaaa
      481 ctaacaatag tgttgccttg attttaagtg gcattgttct ttgaaatcga aattggggat
      541 aacgtcatac cgaaaggtaa acaacttcgg ggaattgccc tggttaaaca tttattaagc
      601 gagataaata ggggatagcg agataggggg cggagaagaa gaagggtgtt aaattgctga
      661 aatctctcaa tctggaagaa acggaataaa ttaactcctt cctgagataa taagatccga
      721 ctctgctatg accccacacg gtactgacct cggcataccc cattggatct ggtgcgaagc
      781 aacaggtcct gaaaccttta tcacgtgtag tagattgacc ttccagcaaa aaaaggcatt
      841 atatattttg ttgttgaagg ggtgagggga ggtgcaggtg gttcttttat tcgtcttgta
      901 gttaattttg ccggggttgc ggagcgtcaa aagtttgccc gatctgatag cttgcaagat
      961 gccaccgctt atccaacgca cttcagagag cttgccgtag aaagaacgtt ttcctcgtag
     1021 tattccagca cttcatggtg aagtcgctat ttcaccgaag ggggggtatt aaggttgcgc
     1081 accccctccc cacaccccag aatcgtttat tggctgggtt caatggcgtt tgagttagca
     1141 cattttttcc ttaaacaccc tccaaacacg gataaaaatg catgtgcatc ctgaaactgg
     1201 tagagatgcg tactccgtgc tccgataata acagtggtgt tggggttgct gttagctcac
     1261 gcactccgtt tttttttcaa ccagcaaaat tcgatgggga gaaacttggg gtactttgcc
     1321 gactcctcca ccatactggt atataaataa tactcgccca cttttcgttt gctgctttta
     1381 tatttcaagg actgaaaaag actcttcttc tactttttca cactatacca cagatatatc
     1441 tactatacat ggcgtcttcg aattcgcgcg tgatcttacg gcattatacg tatgatcggt
     1501 ccacgatcag ctagattatc tagtcagctt gatgtcatag ctgtttcctg aggctcaata
     1561 ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg
     1621 cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     1681 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca
     1741 tattcaacgg gaaacgtctt gcttgaagcc gcgattaaat tccaacatgg atgctgattt
     1801 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     1861 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     1921 tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac
     1981 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg
     2041 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2101 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg
     2161 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc
     2221 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2281 taaactcttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     2341 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     2401 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     2461 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     2521 tcacttgatg ctcgatgagt ttttctaatg aggacctaaa tgtaatcacc tggctcacct
     2581 tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2641 tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2701 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2761 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2821 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2881 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2941 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3001 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     3061 tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc
     3121 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     3181 agaaaaaaag gatctcaaga agatcctttg attttctacc gaagaaaggc ccacccgt
//
