LOCUS       sequence-191944-        2838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PDAS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pDAS2
FEATURES             Location/Qualifiers
     source          1..2838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1134..1150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1134..1150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1198..1229
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1230..1332
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1333..2148
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1406..1425)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2172..2199
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2211..2798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2291..2310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2838,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagcaa tctgtggttt gctaaactgg aagtctggta
      181 aggactctag caagtccgtt actcaaaaag tcataccaag taagattacg taacacctgg
      241 gcatgacttt ctaagttagc aagtcaccaa gagggtccta tttaacgttt ggcggtatct
      301 gaaacacaag acttgcctat cccatagtac atcatattac ctgtcaagct atgctacccc
      361 acagaaatac cccaaaagtt gaagtgaaaa aatgaaaatt actggtaact tcaccccata
      421 acaaacttaa taatttctgt agccaatgaa agtaaacccc attcaatgtt ccgagattta
      481 gtatacttgc ccctataaga aacgaaggat ttcagcttcc ttaccccatg aacagaaatc
      541 ttccatttac cccccactgg agagatccgc ccaaacgaac agataataga aaaaagaaat
      601 tcggacaaat agaacacttt ctcagccaat taaagtcatt ccatgcactc cctttagctg
      661 ccgttccatc cctttgttga gcaacaccat cgttagccag tacgaaagag gaaacttaac
      721 cgataccttg gagaaatcta aggcgcgaat gagtttagcc tagatatcct tagtgaaggg
      781 ttgttccgat acttctccac attcagtcat agatgggcag ctttgttatc atgaagagac
      841 ggaaacgggc attaagggtt aaccgccaaa ttatataaag acaacatgtc cccagtttaa
      901 agtttttctt tcctattctt gtatcctgag tgaccgttgt gtttaatata acaagttcgt
      961 tttaacttaa gaccaaaacc agttacaaca aattataacc cctctaaaca ctaaagttca
     1021 ctcttatcaa actatcaaac atcaaaacat ggcgtcttcg aattcgcgcg tgatcttacg
     1081 gcattatacg tatgatcggt ccacgatcag ctagattatc tagtcagctt gatgtcatag
     1141 ctgtttcctg aggctcaata ctgaccattt aaatcatacc tgacctccat agcagaaagt
     1201 caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac
     1261 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1321 aaggaagcta aaatgagcca tattcaacgg gaaacgtctt gcttgaagcc gcgattaaat
     1381 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     1441 ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     1501 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg
     1561 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     1621 ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca
     1681 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1741 tgtaattgtc cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1801 aataacggtt tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1861 caagtctgga aagaaatgca taaactcttg ccattctcac cggattcagt cgtcactcat
     1921 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1981 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2041 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     2101 gatatgaata aattgcagtt tcacttgatg ctcgatgagt ttttctaatg aggacctaaa
     2161 tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg
     2221 ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg
     2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2401 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2641 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa
     2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc
     2821 gaagaaaggc ccacccgt
//
