LOCUS       sequence-191945-       11669 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Dhr1 with Y2H activation domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 11669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pACT2-Dhr1
FEATURES             Location/Qualifiers
     source          1..11669
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..397
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             443..463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             479..820
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             833..859
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             839..865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    899..923
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4731..4755)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      5144..5331
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5199..5223)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(5352..5385)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(5894..6988)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(6989..7394)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      7871..9213
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8834..8881)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        9478..9582
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9583..9651,9652..10443)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9801..9820)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10614..11202
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11103..11122
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11356..11373
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11388..11528
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11454..11473)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11614..11636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca
       61 atgacaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga cagcaccaac
      121 agatgtcgtt gttccagagc tgatgagggg tatctcgaag cacacgaaac tttttccttc
      181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact
      241 tgaatttgaa ataaaaaaaa gtttgctgtc ttgctatcaa gtataaatag acctgcaatt
      301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg
      361 cacaatattt caagctatac caagcataca atcaactcca agctttgcaa agatggataa
      421 agcggaatta attcccgagc ctccaaaaaa gaagagaaag gtcgaattgg gtaccgccgc
      481 caattttaat caaagtggga atattgctga tagctcattg tccttcactt tcactaacag
      541 tagcaacggt ccgaacctca taacaactca aacaaattct caagcgcttt cacaaccaat
      601 tgcctcctct aacgttcatg ataacttcat gaataatgaa atcacggcta gtaaaattga
      661 tgatggtaat aattcaaaac cactgtcacc tggttggacg gaccaaactg cgtataacgc
      721 gtttggaatc actacaggga tgtttaatac cactacaatg gatgatgtat ataactatct
      781 attcgatgat gaagataccc caccaaaccc aaaaaaagag atctctatgg cttacccata
      841 cgatgttcca gattacgcta gcttgggtgg tcatatggcc atggaggccc cagctttcac
      901 aagtttgtac aaaaaagcag gctacaaaat gggtacttac agaaaaaggt ttaatgaaaa
      961 agccagatcc ggccatatgg ccaaattaaa agaactgaaa agaattagga ataagcagtt
     1021 cactagacag gatgaaaacg atgaaagagt agaaaaccca gattcagctc ccgctgagag
     1081 ttctactacg gagccaaatg ccaatgcgga aattcttgaa cctctcactg aagaagaaaa
     1141 gaaaatgaag aaaagaaaac tacaggagtt atttacccca aaggaatcga aagtttcgag
     1201 attgaagaag aaaagactgg ataaatttat tgaacatcaa ttaaaaagag aggaaagaaa
     1261 gacaattatt ggtaaacttc aagattataa gatagataca agtttgttga caagctctaa
     1321 aaggttagga gagggcaggc agactaaaaa ggaagaattc aaagaagcac tgagtttaga
     1381 acgtcaagga agaggaaatg aacaaacaaa tgaaatatta tacgaagaat atgaaccaaa
     1441 agtttgggat gagtatggtg aaggagggag ctctgaggat gatgacggcg aagatgattt
     1501 cgaggcttca tttggtagta tgccgaaacc aacggacaat gaggagaaga aatcatcagg
     1561 ttttattgat catagaccag caaaatttgg tggatctggt cttagctttg ggtttagcaa
     1621 tataaaagtc ataaacaaag aaagcaaaac tccgaagaag aaatataatt ggagacaacg
     1681 agtagaaatg gaagaattga agaagcatgg gaaagaagat gaaatggatt tcgacacgac
     1741 atctgaagat gatgacgaag aggaggatca ggaagaagaa gacaaaatgc atcctagtga
     1801 aaacccactc gaagaagttg aaagcgcaga tagtgaaact ggctcagaaa aatttgacca
     1861 aaatgatgtt gctaacgaat tcaaggattg ggccaatcaa gaaataaaaa aattggaagg
     1921 tagagatcaa gagctagtta ctcccacttt gaacatcgat tataaaccga taattagaaa
     1981 ggaagattta gatgatggtc tccaagaagc ttatgttcca ataaacgaga attccacccg
     2041 taaagccttc tatgttgaag ttagcagatc agatgaaatt cagaaagcaa gaattcaact
     2101 accagtattt ggtgaagaac acaaaattat ggaggctatt caccataatg atgtagtgat
     2161 tatttgtggt gaaacggggt caggtaagac tacacaagtc ccacaatttt tatatgaggc
     2221 tgggtttggt gccgaagatt ctccagatta cccaggaatg gtgggtatca ctcaaccaag
     2281 aagagtggcc gctgtgtcca tggctgaacg tgtagctaat gaattggggg accatggtca
     2341 taaagtagga taccaaatta ggtttgactc taccgcaaag gaggacacaa aggtcaagtt
     2401 tatgacagat ggtgtcttgc tacgagaaat gatgcacgat ttcaagttga ctaaatattc
     2461 ctccattatc atagatgaag cacatgaaag aaacattaac actgatattt tgatcggtat
     2521 gcttagccgt tgtgttcggt taagagctaa actacataag gagaacccga tcgagcacaa
     2581 gaagctaaaa ctcatcatca tgtcagctac attaagggta tcagatttca gcgaaaacaa
     2641 aacattgttc cccatagcgc cccctgtctt acaggttgat gcaaggcagt ttccagtctc
     2701 tattcacttt aatcgtagaa ctgcattcaa ctatacagac gaggctttca gaaagacttg
     2761 caaaattcac cagaaacttc ctcctggcgc tatattggtg tttttaaccg gtcagcagga
     2821 aattacacac atggtaaaaa ggctaagaaa ggaatttcct tttaagaaaa actccaagta
     2881 caacaaagat ttagaaacac ccgtttctaa aatgggtatc aattcaaaaa caacagattt
     2941 ggaggctgag gatatagact tcagtgtaca agttatcgat caagataagt tcaagagcgc
     3001 gataagatac gaagaagatg aaggtaacag tggaaacggt gaagatgagg aagacgaaga
     3061 ggaagaaggg tttgaagaag tcctaactga agggcaaaca gctaacgatc ctttatatgt
     3121 ccttccactg tattctctat taccaaccaa ggaacaaatg agagtctttc aaaaacctcc
     3181 tcaaggttct aggctttgta ttgttgcaac aaatgttgca gaaacatcgt taactatacc
     3241 tggagttaga tatgttgttg attccgggag gtctaaagag cgtaaatata atgaatcaaa
     3301 tggcgtacag agttttgaag tgggctgggt aagtaaagct agtgccaatc aaagaagcgg
     3361 tagagcaggt cgtactggac caggccattg ttaccgttta tattcgtctg ctgtttttga
     3421 acatgatttc gaacagttct ctaaacctga aatcttaaga atgccagtgg agagcatcgt
     3481 gttacaaatg aagagcatgg ccattcataa tatcattaat tttccatttc caactcctcc
     3541 agatagagtt gccctttcca aggcaataca attactgcaa tatttaggtg ctcttgataa
     3601 taaggagatg attactgaag atggtaaaaa aatgagcctg ttcccacttt cacccagatt
     3661 ttccaaaatg ttacttgttt ctgacgaaaa agcctgcctt ccttatattg tagcaattgt
     3721 tagtgccctt tcggtgggag atccttttat caatgaattt gagttgggaa taaacgaaat
     3781 ttctagaaaa ccaaatccag atgaaaatct agatgataag attagagagc acgatgaatc
     3841 aacaccaggt atggatcctg aactgaaaaa ggaattgcgc agtaaattct ataaaagcag
     3901 gagtcagttt agtaaattag acaaatttag tgacgtattc cgtttattaa gtgttgtaag
     3961 tgcaatggac tacgttccaa aggaacaaaa agaaatattc atgaagaaaa attttttaag
     4021 aggcaaacta atggaagaaa tagtaaaatt gaggaagcag ttaatgtata tcatcaagtc
     4081 caacacttca aaagaaaata ttgccgtagt aattaggaat gaagacttga aaagtgatat
     4141 tccaaacgtc atacaaataa aacttttaaa acaaatgatt tgcgctggtt ttgtcgacca
     4201 tgttgccgta agagcagatg ttttatttcc agatgatgct aagataacca acagaacttc
     4261 cataatcaac attccttata ttcctgtttt ggccacgaga acaccaaata tcgaggactg
     4321 ctttgtctat atccatccga cttcaatttt aaataatttg ggggaaatgc cacccaaata
     4381 catgctctat tattccttgc accttggtgg aaataataaa acaaggatga atactttatg
     4441 tgacatcgca agcaccccct tagcgaacat agcaagaaag ggacttcttt tgacatacag
     4501 taaacctctg actggtcaag gcttgaagac cgttaattta tcacctacgg aacggtattg
     4561 ttatgtggtt cctagatttg gatccacagt tgacaacgat ctcaagattg gttgggattt
     4621 aaatcctata gcagtgcatc agaagaagca aaaaggccag tggacagtca ttaagtttat
     4681 caccagaaag ggcttccaga ccatcacagg tgaagagaaa gaaaaaaaaa acccagcttt
     4741 cttgtacaaa gtggtgagtc gagagatcta tgaatcgtag atactgaaaa accccgcaag
     4801 ttcacttcaa ctgtgcatcg tgcaccatct caatttcttt catttataca tcgttttgcc
     4861 ttcttttatg taactatact cctctaagtt tcaatcttgg ccatgtaacc tctgatctat
     4921 agaatttttt aaatgactag aattaatgcc catctttttt ttggacctaa attcttcatg
     4981 aaaatatatt acgagggctt attcagaagc tttggacttc ttcgccagag gtttggtcaa
     5041 gtctccaatc aaggttgtcg gcttgtctac cttgccagaa atttacgaaa agatggaaaa
     5101 gggtcaaatc gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt
     5161 tatgattttt attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga
     5221 ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt
     5281 gctttctcag gtatagcatg aggtcgctct tattgaccac acctctaccg gcatgccggt
     5341 cgagggacct aataacttcg tatagcatac attatacgaa gttatattaa gggttattga
     5401 atatgatcgg aattggtcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg
     5461 agggaacttt caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa
     5521 tggtcaggtc attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc
     5581 caatatataa attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg
     5641 gtgccctcct ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga
     5701 gccattagta tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga
     5761 taaatgtatg tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta
     5821 atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa
     5881 aaagagaatc tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt
     5941 cggtggtact gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt
     6001 taactgcatc ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca
     6061 ttgcagcaga caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag
     6121 aaccgtggca tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg
     6181 acgcagatgg caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat
     6241 caccaaacat gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga
     6301 tcatggcggc agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga
     6361 tggtttcctc cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca
     6421 aggaccaaat aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga
     6481 ttctttgcac ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt
     6541 cttcctttct cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag
     6601 tacctttagc aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt
     6661 tacatggtct taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt
     6721 caggtctaac actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat
     6781 cagccttctt ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatcgatag
     6841 cagcaccacc aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa
     6901 tagctttaag aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca
     6961 aaacgacgat cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat
     7021 attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta accacctatt
     7081 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt
     7141 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcgctct cacctttcct
     7201 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     7261 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     7321 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     7381 gtattcccac agttccttac cactcttttg ttactctatt gatccagctc agcaaaggca
     7441 gtgtgatcta agattctatc ttcgcgatgt agtaaaacta gctagaccga gaaagagact
     7501 agaaatgcaa aaggcacttc tacaatggct gccatcatta ttatccgatg tgacgctgca
     7561 gcttctcaat gatattcgaa tacgctttga ggagatacag cctaatatcc gacaaactgt
     7621 tttacagatt tacgatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg
     7681 agttttccct gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt
     7741 tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt
     7801 aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca
     7861 tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc
     7921 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa
     7981 agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg
     8041 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     8101 gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca
     8161 tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg
     8221 ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag
     8281 gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta
     8341 ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt
     8401 ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt
     8461 cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga
     8521 aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt
     8581 tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc
     8641 aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat
     8701 agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct
     8761 cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt
     8821 tcaaaagcgc tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt
     8881 caaagcgttt ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct
     8941 cactgttcac gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa
     9001 cggcatagtg cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga
     9061 aaggtagtct agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc
     9121 ttcagcacta ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc
     9181 cttcaatgct atcatttcct ttgatattgg atcatatgca tagtaccgag aaactagtgc
     9241 gaagtagtga tcaggtattg ctgttatctg atgagtatac gttgtcctgg ccacggcaga
     9301 agcacgctta tcgctccaat ttcccacaac attagtcaac tccgttaggc ccttcattga
     9361 aagaaatgag gtcatcaaat gtcttccaat gtgagatttt gggccatttt ttatagcaaa
     9421 gattgaataa ggcgcatttt tcttcaaagc tcaggtggca cttttcgggg aaatgtgcgc
     9481 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9541 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9601 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9661 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9721 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9781 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     9841 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9901 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9961 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
    10021 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
    10081 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
    10141 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
    10201 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    10261 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10321 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10381 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10441 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10501 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10561 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    10621 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    10681 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    10741 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    10801 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10861 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10921 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10981 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    11041 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    11101 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    11161 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    11221 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    11281 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    11341 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
    11401 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
    11461 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
    11521 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    11581 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    11641 caccgtcatc accgaaacgc gcgaggcag
//
