LOCUS       sequence-191946-        3703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea soxB (also 
            known as soxB1-1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Roberts-Galbraith RH, Newmark PA
  TITLE     Follistatin antagonizes activin signaling and acts with notum to 
            direct planarian head regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 
            10.1073/pnas.1214053110. Epub 2013 Jan 7.
  PUBMED    23297191
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1214053110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-22- 22"; volume: "110"; issue: "4"; pages: "1363-8"
COMMENT     Sequence Label: pRRG217-soxB
FEATURES             Location/Qualifiers
     source          1..3703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     491..510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     744..761
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(773..820)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        834..852
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        856..874
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     856..873
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1354..1372)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1376..1394)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1408..1455
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1862..2656
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1916..1935)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2526..2545
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2674..2742,2743..3534)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2892..2911)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
       61 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcacgc gtcaaaaaac
      781 ccctcaagac ccgtttagag gccccaaggg gttatgctag gcgcgccgga tcctaatacg
      841 actcactata gggagattta ggtgacacta tagaagtgac ctttactcat ccgccctttc
      901 atcctactat cagaaattaa gaaatacctc tgtagatttt gatgttccaa acgatgttta
      961 tgacaacatt tattccaact ctctccaatc ggaaaagaat ccctatggct cgtacttttc
     1021 cagtccgact ctcgattcga aatcttttga cgattcgaaa ctatcgagtc aaacaaataa
     1081 tttcgattgt ttaaaacagt tttctcaaat tgacacaaac accttgcaaa cagttcaaac
     1141 gattgctgct ttgcattctc ggcatcattt gagctcaagt tctagtattg atggtaataa
     1201 cgttgaaccc aatcacaaat ccgattcgtc acccaatcca ccaacgactc agtgtcaagg
     1261 tgctcacagt tttcccactt taaacaattc atcttgtcaa cagaggcctg gattacgtac
     1321 tccgaagtcc gaagacattg atcggaaggt ctccctttag tgagggttaa ttctccctat
     1381 agtgagtcgt attaggatcc ggcgcgccta gcataacccc ttggggcctc taaacgggtc
     1441 ttgaggggtt ttttgacgcg tcgatttcgg cctattggtt aaaaaatgag ctgatttaac
     1501 aaaaatttaa cgcgaatttt aacaaaattc agggcgcaag ggctgctaaa ggaagcggaa
     1561 cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc
     1621 tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac
     1681 atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg
     1741 ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc
     1801 aatgatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg
     1861 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     1921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     1981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     2041 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     2101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     2161 ggatctcctg tcatcccacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     2221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     2281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     2341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
     2401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     2461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     2521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     2581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     2641 tgacgagttc ttctgaattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     2701 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     2761 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     2821 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2881 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2941 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     3001 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     3061 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     3121 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     3181 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     3241 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     3301 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     3361 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     3421 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagccaggc aactatggat
     3481 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3541 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3601 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat ctt
//
