LOCUS       sequence-191947-        2861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PAOX1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pAOX1
FEATURES             Location/Qualifiers
     source          1..2861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1157..1173)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1157..1173)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1221..1252
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1253..1355
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1356..2171
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1429..1448)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2195..2222
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2234..2821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2314..2333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2861,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagaga cgaaaggttg aatgaaacct ttttgccatc
      181 cgacatccac aggtccattc tcacacataa gtgccaaacg caacaggagg ggatacacta
      241 gcagcagacc gttgcaaacg caggacctcc actcctcttc tcctcaacac ccacttttgc
      301 catcgaaaaa ccagcccagt tattgggctt gattggagct cgctcattcc aattccttct
      361 actaggctac taacaccgtg actttattag cctgtctatc ctggcccccc tggcgaggtt
      421 catgtttgtt tatttccgaa tgcaacaagc tccgcattac acccgaacat cactccagat
      481 gagggctttc tgagtgtggg gtcaaatagt ttcatgttcc ccaaatggcc caaaactgac
      541 agtttaaacg ctgtcttgga acctaatatg acaaaagcgt gatctcatcc aagatgaact
      601 aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg
      661 cataccgttt gtcttgtttg gtattgattg acgaatgctc aaaataatct cattaatgct
      721 tagcgcagtc tctctatcgc ttctgaaccc cggtgcacct gtgccgaaac gcaaatgggg
      781 aaacacccgc tttttggatg attatgcatt gtctccacat tgtatgcttc caagattctg
      841 gtgggaatac tgctgatagc ctaacgttca tgatcaaaat ttaactgttc taacccctac
      901 ttgacagcaa tatataaaca gaaggaagct gccctgtctt aaaccttttt ttttatcatc
      961 attattagct tactttcata attgcgactg gttccaattg acaagctttt gattttaacg
     1021 acttttaacg acaacttgag aagatcaaaa aacaactaat tattcgaaac catggcgtct
     1081 tcgaattcgc gcgtgatctt acggcattat acgtatgatc ggtccacgat cagctagatt
     1141 atctagtcag cttgatgtca tagctgtttc ctgaggctca atactgacca tttaaatcat
     1201 acctgacctc catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc
     1261 acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg
     1321 agttatcgag attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt
     1381 cttgcttgaa gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1441 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg
     1501 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1561 tggtcaggct aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1621 gtactcctga tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg
     1681 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1741 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc
     1801 tcgctcaggc gcaatcacga atgaataacg gtttggttgg tgcgagtgat tttgatgacg
     1861 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactc ttgccattct
     1921 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1981 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     2041 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     2101 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcacttg atgctcgatg
     2161 agtttttcta atgaggacct aaatgtaatc acctggctca ccttcgggtg ggcctttctg
     2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct
     2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2521 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2701 tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2821 agaagatcct ttgattttct accgaagaaa ggcccacccg t
//
