LOCUS       sequence-191949-        2487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: promoter PDAS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_12_pDAS1
FEATURES             Location/Qualifiers
     source          1..2487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(783..799)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      847..878
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        879..981
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             982..1797
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1055..1074)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1821..1848
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1860..2447)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1940..1959)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2487,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcggagaat gatataaaca acaattgagt gacaggtcta
      181 ctttgttctc aaaaggccat aaccatctgt ttgcatctct tatcaccaca ccatcctcct
      241 catctggcct tcaattgtgg ggaacaacta gcatcccaac accagactaa ctccacccag
      301 atgaaaccag ttgtcgctta ccagtcaatg aatgttgagc taacgttcct tgaaactcga
      361 atgatcccag ccttgctgcg tatcatccct ccgctattcc gccgcttgct ccaaccatgt
      421 ttccgccttt ttcgaacaag ttcaaatacc tatctttggc aggacttttc ctcctgcctt
      481 ttttagcctc agttctcggt tagcctctag gcaaattctg ttcttcatac ctatatcaac
      541 ttttcatcag atagcctttg ggttcaaaaa agaactaaag caggatgcct gatatataaa
      601 tcccagatga tctgcttttg aaactatttt cagtatcttg attcgtttac ttacaaacaa
      661 ctattgttga ttttatctgg agaataatcg aacaaacatg gcgtcttcga attcgcgcgt
      721 gatcttacgg cattatacgt atgatcggtc cacgatcagc tagattatct agtcagcttg
      781 atgtcatagc tgtttcctga ggctcaatac tgaccattta aatcatacct gacctccata
      841 gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc
      901 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
      961 tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg cttgaagccg
     1021 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1081 gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt
     1141 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac
     1201 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1261 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
     1321 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1381 attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1441 tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg
     1501 cctgttgaac aagtctggaa agaaatgcat aaactcttgc cattctcacc ggattcagtc
     1561 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1621 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1681 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1741 gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctaatga
     1801 ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt
     1861 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg
     1921 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1981 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     2041 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     2101 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     2161 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     2221 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     2281 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2341 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2401 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2461 ttttctaccg aagaaaggcc cacccgt
//
