LOCUS       sequence-191953-        4169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea prohormone 
            convertase 2 (PC2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Roberts-Galbraith RH, Newmark PA
  TITLE     Follistatin antagonizes activin signaling and acts with notum to 
            direct planarian head regeneration.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jan 22;110(4):1363-8. doi: 
            10.1073/pnas.1214053110. Epub 2013 Jan 7.
  PUBMED    23297191
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1214053110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-01-22- 22"; volume: "110"; issue: "4"; pages: "1363-8"
COMMENT     Sequence Label: pRRG389-PC2
FEATURES             Location/Qualifiers
     source          1..4169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     498..517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     751..768
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(780..827)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        841..859
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        863..881
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     863..880
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1827..1845)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1849..1867)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1881..1928
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2335..3129
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2389..2408)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2999..3018
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3147..3215,3216..4007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3365..3384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
       61 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcacgcgtc
      781 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctaggcg cgccggatcc
      841 taatacgact cactataggg agatttaggt gacactatag aagtgacctt cactccaaca
      901 ccacaaatgc cattatcacg ttcggcggaa atctcaccgg cacatcttgt tccgtgactg
      961 ttaaaccaat cgtcagtata tcgagggaac gggtaagggt cattgctact gaaatcataa
     1021 ctgctttctg cgttataatt ttttgataaa tccgggtgaa gatagtctat tccatcatcc
     1081 atgattgcag tcgtgacccc tttgccggtt ataccttgag cccacgctga taatacatta
     1141 agatcaagat ctttcactcc atcagcttga ccggtatttc tgatgtacca ttctttagaa
     1201 aatagaggat cagtaggtaa tttcaaatcc actttaatgt tgtcagactc tcccagaccg
     1261 ggaattcctt ttttattaat cgttggcagt gggacacgac cttgtttttt aattggctta
     1321 tagcctctct ttcgtctcaa aaacccttca acttgttttg cgtttttaac aagatggtga
     1381 gccaatagaa ttttgtgttg actccaactt ggaactgaac taacgtgagg aacggttttg
     1441 tgatggaaaa caaattcatt tggagaattc agatggtttc gtaagaccga gaaaccagtt
     1501 tcctgagcta ctttatttgc cgattcttga gttgcaggat catgaaggtg gacatgccat
     1561 tgattggtga caatttttcc acaaactaca tattcgcata gaaacaaaag gatccatgta
     1621 atcttcatag taagccgtta gttttttctg aatcggactt ttagaatgtc cactagaaac
     1681 cttcagattt tctttatcat tttttctaat gattgtatca ttatttttgg taatttcttt
     1741 gtaatgagat ttgtaatgaa cttgcaaact atttttaagt aaaaatgatt ttgcacatat
     1801 ggtacaggtg tgcaacgaaa ggtctccctt tagtgagggt taattctccc tatagtgagt
     1861 cgtattagga tccggcgcgc ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     1921 gttttttgac gcgtcgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     1981 taacgcgaat tttaacaaaa ttcagggcgc aagggctgct aaaggaagcg gaacacgtag
     2041 aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg
     2101 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
     2161 tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc
     2221 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaatgatc
     2281 tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     2341 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     2401 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     2461 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     2521 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     2581 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     2641 ctgtcatccc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     2701 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     2761 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     2821 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     2881 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     2941 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     3001 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     3061 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     3121 ttcttctgaa ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     3181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     3481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     3541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     3601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     3661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     3721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     3781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     3841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     3901 agccctcccg tatcgtagtt atctacacga cggggagcca ggcaactatg gatgaacgaa
     3961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4141 gagcgtcaga ccccgtagaa aagatcaaa
//
