LOCUS       sequence-191959-        2406 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: RPS2tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_RPS2tt
FEATURES             Location/Qualifiers
     source          1..2406
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(702..719)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(702..718)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      759..1346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1247..1266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1358..1385)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1409..2224)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2132..2151
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(2225..2327)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(2328..2359)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 caggaaacag ctatgacatc aagctgacta gataatctag ctgatcgtgg accgatcata
       61 cgtataatgc cgtaagatca cgcgcgaatt cgaagacgca gcgctctaat ccggactgtc
      121 caaaggctca tcgatcacct gaacacaaga tcaccgaaga agagaaacaa caaaaacaac
      181 agagggcttt ggaacaatgt cgatttggta aatcatgccg taattacaag tgcccaagac
      241 gtcacgcaac tactcctgta ctgtgtcgtg aaggcaacag ttgtaccaga ccaaactgtt
      301 ttttcactca tccaattgat gaggattgta agtttgcagc tggatgccgc aaccctaact
      361 gcccatttaa acacccagaa ggtcgtgaga ttagtgctgg tgcaccggtg actaatgctg
      421 tgtggactcc cgaaactggc gctactagtg aaagacagtt tgctgtccca gaggaccaga
      481 ttatggaaca agttccgttg caaaactaat aactaccaca tttgtacatt aagtaaagtt
      541 tgaacaggtg aaatagatta atatttgact aaagcgcgtc ttcgaattcg aggccgcata
      601 tcattcagga cgagcctcag actccagcgt aactggactg caatcaactc actggctcac
      661 cttccggtcc acgatcagct agaatcaagc tgactagata aactggccgt cgttttacac
      721 gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc
      781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      841 caagagctac caactctttt tccgaggtaa ctggcttcag cagagcgcag ataccaaata
      901 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
      961 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1021 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1081 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1141 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1201 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1261 atctttatag tcctgtcggg tttcgccacc tctgacttga gcatcgattt ttgtgatgct
     1321 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt
     1381 gagccaggtg attacattta ggtcctcatt agaaaaactc atcgagcatc aagtgaaact
     1441 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1501 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1561 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1621 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaag agtttatgca
     1681 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcac
     1741 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgccgt
     1801 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     1861 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg
     1921 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     1981 gaagaggcat aaattccgtc agccagttta gcctgaccat ctcatctgta acatcattgg
     2041 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     2101 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2161 cagcatccat gttggaattt aatcgcggct tcaagcaaga cgtttcccgt tgaatatggc
     2221 tcattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     2281 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaagt caaaagcctc
     2341 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgatttaaat ggtcagtatt
     2401 gagcct
//
