LOCUS       sequence-191960-        2437 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: RPP1Btt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_RPP1Btt
FEATURES             Location/Qualifiers
     source          1..2437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      797..828
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        829..931
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             932..1747
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1005..1024)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1771..1798
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1810..2397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1890..1909)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2437,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttgga tgatacttta attgatgcat gtgaagaatt
      181 gaagaaacag taatctagta aaatataaga aagtcctgaa agtagttcat ttagtaagct
      241 atgaacccaa gttggggtaa ccgatttttc aggtaaacag ctgtagtgag gagacatctc
      301 aaatcgcctg agaagaaaaa tatctatgag acaactacaa tgcgaagaat gtgttcaaat
      361 gctctattat tcactagttt acctattcaa tgaccaactc ctgggaggct tcgttaccga
      421 taattggtaa cttaactagt acgtccttca tcaactcgta gacagtgaaa gtgacggctt
      481 gtcccggagc aacacgcatg atacgcggcg tgataccttt gtacagagca cgcatacctt
      541 cctcttggat caatctcttg gaaatacgaa gaatacgggc cacaccagac tcattggtga
      601 caccagtctc tctctgcata cgagtcttga tggtatctag aggagccgct gcgtcttcga
      661 attcgcgcgt gatcttacgg cattatacgt atgatcggtc cacgatcagc tagattatct
      721 agtcagcttg atgtcatagc tgtttcctga ggctcaatac tgaccattta aatcatacct
      781 gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg atcggcacgt
      841 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
      901 atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg aaacgtcttg
      961 cttgaagccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     1021 cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc
     1081 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     1141 caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     1201 tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt
     1261 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     1321 gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc
     1381 tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg atgacgagcg
     1441 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaactcttgc cattctcacc
     1501 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     1561 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     1621 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     1681 atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc tcgatgagtt
     1741 tttctaatga ggacctaaat gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt
     1801 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gatgctcaag
     1861 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     1921 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     1981 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2041 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2101 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2161 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2221 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2281 gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2341 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2401 gatcctttga ttttctaccg aagaaaggcc cacccgt
//
