LOCUS       sequence-191961-        2425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: RPS17Btt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_RPS17Btt
FEATURES             Location/Qualifiers
     source          1..2425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(721..737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      785..816
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        817..919
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             920..1735
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(993..1012)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1759..1786
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1798..2385)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1878..1897)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2425,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttggg tttcatagct actgttagta gatcaataca
      181 cacattgtat agttaaaagt gataaccata atttccttgc acatccttcg aaatttgaaa
      241 attctgatca tcgattgtat tgacctcttg agctggttag aaacccaccg gacaaaggca
      301 tcctattctg aaaagtggca atatggcaag gtaactgagt accactggtt ggtatgtttc
      361 cctttttttt ttacttatcg gcgtgtttgt aatataaagt tctctgttta caaaactcat
      421 ttactgaaaa cagatgctgg tatttacttt gaaaaagtta gattacttgc cagttgatta
      481 aaattcgtgg cagtcctgtg tatattctac aattcgtcat ctttggccaa cacgacaaag
      541 gaaagtcaaa cggatcaaaa aaaaaaaaaa tattgaatga atttgattac catttacttt
      601 gactataaac aaccagccaa cctgaatgat caatcgctgc gtcttcgaat tcgcgcgtga
      661 tcttacggca ttatacgtat gatcggtcca cgatcagcta gattatctag tcagcttgat
      721 gtcatagctg tttcctgagg ctcaatactg accatttaaa tcatacctga cctccatagc
      781 agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa gaggttccaa
      841 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      901 aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct tgaagccgcg
      961 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     1021 gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct
     1081 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg
     1141 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     1201 atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc
     1261 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     1321 tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc
     1381 acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc
     1441 tgttgaacaa gtctggaaag aaatgcataa actcttgcca ttctcaccgg attcagtcgt
     1501 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     1561 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     1621 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     1681 taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt tctaatgagg
     1741 acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt
     1801 ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg
     1861 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1921 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1981 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2041 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2101 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2161 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2221 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc
     2281 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     2341 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt
     2401 ttctaccgaa gaaaggccca cccgt
//
