LOCUS       sequence-191963-        2413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: chr4_0883tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_chr4_0883tt
FEATURES             Location/Qualifiers
     source          1..2413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(709..725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      773..804
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        805..907
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             908..1723
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(981..1000)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1747..1774
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1786..2373)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1866..1885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2413,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttctg tttgtcctta tacatcattc caaggttaga
      181 aaaggccgga aaacagacat ttttatttca tagaatattg cagtaggata tatccgctac
      241 actcgcttat cagcccattt taatttaata ttttagttta attttttaac tgatcagtta
      301 gccttctttc agcaagggct gaaagatgtc gtgcgtaaac tcaaatagtt acttacttta
      361 gcaatgatca tttgtttgct cttgttgtag ccaagtttct cggcagacaa aagccgctcg
      421 ttgaagtcca ttcggaaagt caaaggtcat cgaaagtatt tcgaaggggc tgaatctcga
      481 agggggctga atctgcaggg actttacgtg tcttatcgat caaaagatta attctaaaac
      541 aggcaagaaa tgcgcgaatt aatcttacaa ctatagttgc tgctgctttt tagagcggtc
      601 aacgtttctt accaggttgt ttcgctgcgt cttcgaattc gcgcgtgatc ttacggcatt
      661 atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt catagctgtt
      721 tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag aaagtcaaaa
      781 gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa
      841 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
      901 agctaaaatg agccatattc aacgggaaac gtcttgcttg aagccgcgat taaattccaa
      961 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     1021 gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     1081 aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc tgacggaatt
     1141 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     1201 cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     1261 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     1321 ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     1381 cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     1441 ctggaaagaa atgcataaac tcttgccatt ctcaccggat tcagtcgtca ctcatggtga
     1501 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     1561 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     1621 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     1681 gaataaattg cagtttcact tgatgctcga tgagtttttc taatgaggac ctaaatgtaa
     1741 tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc ataggctccg
     1801 cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg
     1861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2221 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg aaaaagagtt
     2281 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2341 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt ctaccgaaga
     2401 aaggcccacc cgt
//
