LOCUS       sequence-19198-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf115
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             360..401
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             411..428
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(451..468)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    457..683
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catggagcaa acattaacgc ggttacacac
       61 ttaccttcaa cagtatacca aacatagtcc tcgtgtggtg tatgctcttt tatctcgagg
      121 agccgatacg aggatacgta ataatcttga ttgtacaccc atcatggaac gattgtgcaa
      181 caggtcatat tctcataatg ttactcaatt ggcacgaaca aaaggaagaa ggacaacatc
      241 tactttatct attcataaaa cataagcaat tcccggggat ccgaataaag ggtcaagaca
      301 attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaag
      361 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
      421 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      481 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      541 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
      601 gggggtgggg tgggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
      661 tggggatgcg gtgggctcta tggcttctga ggcggaaag
//
