LOCUS       sequence-19199-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf116
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             472..513
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             523..540
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(563..580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    569..680
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaccaccatg ctcttttatc
       61 tcgaggagcc gatacgagga tacgtaataa tcttgattgt acacccatca tggaacgatt
      121 gtgcaacagg tcatattctc ataatgttac tcaattggca cgaacaaaag gaagaaggac
      181 aacatctact ttatctattc ataaaacata atcaaggata cactctcaat atactacggt
      241 atctactaga taggttcgac attcagaaag acgaatacta taataccgcc tttcaaaatt
      301 gtaacaacaa tgttgcctca tacatcggat acgacatcaa ccttccgact aaagacggta
      361 ttcgacttgg tgtttgagca attcccgggg atccgaataa agggtcaaga caattctgca
      421 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct
      481 atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat
      541 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      661 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attc
//
