LOCUS       sequence-191992-        2585 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid vector pDGB2-alpha-1, adapted for S. cerevisiae genome 
            integration by flanking the GB cassette with recombination arms of 
            homology for integration at the YPRC-Delta-15 solo LTR loci.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, 
            Navarro-Rodriguez M, Rubio LM, Caro E
  TITLE     Adaptation of the GoldenBraid modular cloning system and creation of
            a toolkit for the expression of heterologous proteins in yeast 
            mitochondria.
  JOURNAL   BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 
            10.1186/s12896-017-0393-y.
  PUBMED    29132331
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2017-11-13"; volume: "17(1):80. doi"; pages: " 
            10.1186/s12896-017-0393-y"
COMMENT     Sequence Label: YPRC-Delta-15-alpha-1
FEATURES             Location/Qualifiers
     source          1..2585
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1051..1374)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     1056..1075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1269..1291
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1283..1300
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1284..1300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1301..1357
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1370..1386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1370..1386)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1383..1405)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1394..1410
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1418..1424,1425..1442,1443..1448))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1463..1484
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcggccgcta gggataacag ggtaatcgag atagtttgac gttcgttttt tactttgaat
       61 atactcgtag tctttttact ttttgagtat aaaaaaaaaa tgactagcaa aataaaatta
      121 gtagtctaaa aaagaaagct cgcactcagg atcgaactaa ggaccaacag atttgcaatc
      181 tgctgcgcta ccactgcgcc atacgagctt ttgaattatg gtaattttga ttatcctaga
      241 atgttatatc tcaatatctc aatatatttt ggacatctat gaaacaccca taaagcagcc
      301 gctaccaaac agacaagatt cagtatgtaa ggtaaatacc tttttgcaca gttaaactac
      361 ccaaacttat taaagcttga taaattactg aaattccacc tttcagttag attcaggcct
      421 catatagatt agatataggg tacgtaacat tctgtcaacc aagttgttgg aatgaaagtc
      481 taaaatgtca tctattcggt agcactcatg ttactagtat actgtcacat gcggtgtaac
      541 gtggggacat aaaacagaca tcaaatataa tggaagctga aatgcaaaga tcgataatgt
      601 aataggaatg aaacatataa aacgaaagga gaagtaatgg taatattagt atgtagaaat
      661 accgattcaa ttttggggat tcttatattc tcgagagaat ttctagtata atctgtatac
      721 ataatattat aggctttacc aacaatggaa tttcgacaat tatcatatta ttcaccaatt
      781 aatcacaagt tggtaatgag tttgataaca agttactttc ttaacaacgt tagtatcgtc
      841 aaaacactcg gttttactcg agcttgtagc acaataatac cgtgtagagt tctgtattgt
      901 tcttcttagt gcttgtatat gctcatcccg accttccatt gaattcgtct caggagagag
      961 acccacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1021 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     1081 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     1141 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     1201 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     1261 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc tcggtacccg
     1321 gggatcctct agagtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt
     1381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1501 tgcccgcttt ccagtcggga aacctgtcgt gccagctggg gtctctcgct gtcatgagac
     1561 gaattctttg cgaaacccta tgctctgttg ttcggatttg aaattttaaa actacattaa
     1621 tgtgttagtt tttctttctt tctttctttg tcttgacgtg atttggactt ctgtcttgca
     1681 ttcgcgtcca ttcatctgac ccaatattcc ttttggtttt gttatcctta taaaaagaaa
     1741 ggaagcttct tagagggaaa aaaatgatga agagtaatgc caaaatataa ataaataaat
     1801 aaatatgaaa atcattttct atttttaata gaataagaag agcatcttaa gattacaatt
     1861 tcaagaaata gtttacacag tatatccaat aactccaata aactactttc ctatacaaat
     1921 ttctatggtg ggattaatag taaaacttct gtacttctct aattcaccaa gaaattaagg
     1981 taaacatctg gtaagcacta tccagctttt tgctattaca catatggctt ttctgcaatc
     2041 atttcttccc attttgtctc aagccgttag tcttgaaacc acaggcggag tagagttact
     2101 tgatgcggta ttttacatgc cttttttcac tgcaaaaaaa atgaaataca tatttacacg
     2161 atttgcagga cagtttacga tagtgagtat gcagaatagt taacaccttt gttttatcct
     2221 tttgtgtctt aattatatga tataaaggcg cctggcgtta tcggatagta atagatgcta
     2281 gttatcaaca tttcacaatt gaaggaaata aagttgaagt actcaacaaa aacttacttc
     2341 agaattaaat ttttgggggg aacataggca tcctatgaca ggtgaccaca agcccctcaa
     2401 cgcaatctaa tattttacaa agtggtaaaa ttctttcgtt cttcgtttta atatacagtc
     2461 atttattgat tctattacat taatattcct acgcttcggc tcacataatt aacaggactt
     2521 cgagtccgtt aaacttggga tcaactaatt tctacggatt agggataaca gggtaatgcg
     2581 gccgc
//
