LOCUS       sequence-192017-        2104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: RPS3tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_RPS3tt
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(400..416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      464..495
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        496..598
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             599..1414
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(672..691)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1438..1465
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1477..2064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1557..1576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2104,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttctg tttaattctg taggataaac gaaataactg
      181 tatctttata atcatcctta agatagccct ggactcgtca gacattagtt cgcgcacatt
      241 attttcctcc actgtctgca aacagccaca cggcatttcc ctctctagtt tcatttatca
      301 aagtagcaca acacgctgcg tcttcgaatt cgcgcgtgat cttacggcat tatacgtatg
      361 atcggtccac gatcagctag attatctagt cagcttgatg tcatagctgt ttcctgaggc
      421 tcaatactga ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac
      481 cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag
      541 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
      601 gagccatatt caacgggaaa cgtcttgctt gaagccgcga ttaaattcca acatggatgc
      661 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
      721 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
      781 tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct
      841 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
      901 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
      961 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1021 taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     1081 tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     1141 aatgcataaa ctcttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     1201 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     1261 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     1321 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     1381 gcagtttcac ttgatgctcg atgagttttt ctaatgagga cctaaatgta atcacctggc
     1441 tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc gcccccctga
     1501 cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag gactataaag
     1561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1921 agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt tggtagctct
     1981 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2041 acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccgaag aaaggcccac
     2101 ccgt
//
