LOCUS       sequence-192019-        2434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: RPL2Att.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_RPL2Att
FEATURES             Location/Qualifiers
     source          1..2434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(730..746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      794..825
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        826..928
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             929..1744
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1002..1021)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1768..1795
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1807..2394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1887..1906)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2434,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagttta tctagtcagc ttgattctag ctgatcgtgg accggaaggt
       61 gagccagtga gttgattgca gtccagttac gctggagtct gaggctcgtc ctgaatgata
      121 tgcggcctcg aattcgaaga cgcgcttcta tgtaactaac gaaacagcat gtactaatag
      181 aaccgtatcg agaatattta tttaggtgag tagtaggagt gaaccagaca gtcaatttag
      241 tgagctgtcc cagcttttgt gcattccaga attgccggtc aaattggtta tgggttatgg
      301 ggcttttccg attgaggttc agtttctgcg gttatctctt tcttgacctg gtcttttaca
      361 ggctgttctt tctccccatg attattcttt agctgaagat accgcttagc ctgataatgt
      421 cgtcgttttg taatcaaaat ctttagttgg gcatcgtctg aggtttcctt tggcttctgg
      481 ggttgttagt aggaacgtag gaaccatagt aacttttaca catacattct tatgattgcg
      541 aagtaagctg agtctgctgc ttggctcccg aagtactttc tctttctcta ccggttgatt
      601 ctccttctgg tgctcctaaa cgattgtgtt agaagggatt gaccgctgcg tcttcgaatt
      661 cgcgcgtgat cttacggcat tatacgtatg atcggtccac gatcagctag attatctagt
      721 cagcttgatg tcatagctgt ttcctgaggc tcaatactga ccatttaaat catacctgac
      781 ctccatagca gaaagtcaaa agcctccgac cggaggcttt tgacttgatc ggcacgtaag
      841 aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc
      901 gagattttca ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctt
      961 gaagccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1021 taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga
     1081 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     1141 gctaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     1201 tgatgatgca tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga
     1261 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     1321 gcattcgatt cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgctca
     1381 ggcgcaatca cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa
     1441 tggctggcct gttgaacaag tctggaaaga aatgcataaa ctcttgccat tctcaccgga
     1501 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     1561 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     1621 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     1681 tggtattgat aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt
     1741 ctaatgagga cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct
     1801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca
     1861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1981 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2221 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2281 agttacctcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2401 cctttgattt tctaccgaag aaaggcccac ccgt
//
