LOCUS       sequence-19205-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf128
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             361..402
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             412..429
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(452..469)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    458..686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc caccgaattc cagctgacca ccatgttaaa gttaaaggat atagcgatgg
       61 ctctactaga agccactgga tttagcaata taaatgactt taatatattc agctatatga
      121 aatccaaaaa tgtagacgtt gacttgataa aggtgttggt agaacatgga tttgacttga
      181 gtgttaaatg tgaaaaccat cgttcagtta tagaaaatta tgtaatgaca atgatcctgt
      241 tcctaaaatt attgatttgt tcataggcaa ttcccgggga tccgaataaa gggtcaagac
      301 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa
      361 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac
      421 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
      481 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
      541 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
      601 gggggttggg gggtggggca ggacagcaag gggggaggat tgggaagaca atagcaggca
      661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      721 ggggtat
//
