LOCUS       sequence-192066-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fusion gene cloning. Recombine Gene B into this Gateway Entry 
            Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Lu H, Villafane N, Dogruluk T, Grzeskowiak CL, Kong K, Tsang YH, 
            Zagorodna O, Pantazi A, Yang L, Neill NJ, Kim YW, Creighton CJ, 
            Verhaak RG, Mills GB, Park P, Kucherlapati RS, Scott KL
  TITLE     Engineering and Functional Characterization of Fusion Genes 
            Identifies Novel Oncogenic Drivers of Cancer.
  JOURNAL   Cancer Res. 2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745.
  PUBMED    28512244
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 May 16. pii: canres.2745.2016. doi: 
            10.1158/0008-5472.CAN-16-2745."
COMMENT     Sequence Label: pFUSE-B_P2rP4
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(116..215)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(385..690)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(487..506)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 tctaagtagt tgattcatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
       61 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
      121 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
      181 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat ccgaagaaat
      241 atttcacaac tcttatactt ttctcttaca agtcgttcgg cttcatctgg attttcagcc
      301 tctatactta ctaaacgtga taaagtttct gtaatttcta ctgtatcgac ctgcagactg
      361 gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa tggcgttttt
      421 gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg agaccggcac
      481 actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca ccaccgggta
      541 aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga tcaccatccg
      601 tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat aacggctctc
      661 tcttttatag gtgtaaacct taaactgcat ttcaccagcc cctgttctcg tcagcaaaag
      721 agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt tccgctttcc
      781 agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc agaccggaga
      841 tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg tcactgtaat
      901 acgctgcttc atagcatacc tctttttgac atacttcggg tatacatatc agtatatatt
      961 cttataccgc aaaaatcagc gcgcaaatac gcatactg
//
