LOCUS       sequence-19207-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf130
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             677..718
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             728..745
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gtaccgagct cggatccagt acccttcacc gaattccagc tgaccaccat gtcttcgatt
       61 agatttattg catgtctata tctcatttcc atcttcggaa attgtcatga ggatccatat
      121 tatcaaccat ttaataaatt aaacattact ctagatatat acacttatga ggatctagta
      181 ccatacaccg tagacaatga cacaacttct ttcgttaaga tatactttaa aaaattttgg
      241 attactgtta tgactaaatg gtgtgctccg tttattgata ccgttagcgt atacacatcg
      301 catgataatc tgaatataga attttatact agagacgaat atgatacaca aagcgaggat
      361 aaaatttgta ccattgatgt taaagcacga tgcaaccatc taacaaaacc agaagttaca
      421 gtacaaaaag aagcgtacag atattcatta tcttctgacc tatcgtgttt tgattctata
      481 gatctagaca ttgatctgat cgaaactaat agtactgaca ctacagtact gaaatcatat
      541 gagctcatgc ttcccaaacg tgctaaatcc atacataact gagcaattcc cggggatccg
      601 aataaagggt caagacaatt ctgcagatat ccagcacagt ggcggccgct cgagtctaga
      661 gggcccgcgg ttcgaaggta agcctatccc taaccctctc ctcggtctcg attctacgcg
      721 tacgggtcat catcaccatc accattgagt ttaaacc
//
