LOCUS       sequence-192075-       13954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, 
            Hung T, Argani P, Rinn JL, Wang Y, Brzoska P, Kong B, Li R, West RB,
            van de Vijver MJ, Sukumar S, Chang HY
  TITLE     Long non-coding RNA HOTAIR reprograms chromatin state to promote 
            cancer metastasis.
  JOURNAL   Nature. 2010 Apr 15. 464(7291):1071-6.
  PUBMED    20393566
REFERENCE   2  (bases 1 to 13954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Apr 15. 464(7291):1071-6."
COMMENT     Sequence Label: LZRS-Flag_EZH2_ER
FEATURES             Location/Qualifiers
     source          1..13954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     345..368
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     rep_origin      2435..4224
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(4718..5509,5510..5578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5341..5360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5579..5683)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             6220..6689
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    6754..7111
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             7176..7592
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7522..7544
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7560..7576
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    7602..7976
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     7914..7930
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    8006..8030
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8033..8042
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8042..8065
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(11286..11310)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             11623..12092
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(12222..12239)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12393..12981)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12473..12492)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    13104..13238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13128..13147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13182..13201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(13551..13571)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 gcgccggccc ggcgcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
       61 gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc
      121 cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt
      181 cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta
      241 gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg
      301 gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa
      361 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttaattctca
      421 tgtttgacag cttatcatcg ataagctgat cctcacaggc cgcacccagc ttttcttccg
      481 ttgccccagt agcatctctg tctggtgacc ttgaagagga agaggagggg tcccgagaat
      541 ccccatccct accgtccagc aaaaaggggg acgaggaatt tgaggcctgg cttgaggctc
      601 aggacgcaaa tcttgaggat gttcagcggg agttttccgg gctgcgagta attggtgatg
      661 aggacgagga tggttcggag gatggggaat tttcagacct ggatctgtct gacagcgacc
      721 atgaagggga tgagggtggg ggggctgttg gagggggcag gagtctgcac tccctgtatt
      781 cactgagcgt cgtctaataa agatgtctat tgatctcttt tagtgtgaat catgtctgac
      841 gaggggccag gtacaggacc tggaaatggc ctaggagaga agggagacac atctggacca
      901 gaaggctccg gcggcagtgg acctcaaaga agagggggtg ataaccatgg acgaggacgg
      961 ggaagaggac gaggacgagg aggcggaaga ccaggagccc cgggcggctc aggatcaggg
     1021 ccaagacata gagatggtgt ccggagaccc caaaaacgtc caagttgcat tggctgcaaa
     1081 gggacccacg gtggaacagg agcaggagca ggagcgggag gggcaggagc aggaggggca
     1141 ggagcaggag gaggggcagg agcaggaggg gcaggagcag gaggtggagg ccggggtcga
     1201 ggaggcagtg gaggccgggg tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga
     1261 ggccgccggg gtagaggacg tgaaagagcc agggggggaa gtcgtgaaag agccaggggg
     1321 agaggtcgtg gacgtggaga aaagaggccc aggagtccca gtagtcagtc atcatcatcc
     1381 gggtctccac cgcgcaggcc ccctccaggt agaaggccat ttttccaccc tgtaggggaa
     1441 gccgattatt ttgaatacca ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg
     1501 ggagcgatag agcagggccc cgcagatgac ccaggagaag gcccaagcac tggaccccgg
     1561 ggtcagggtg atggaggcag gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa
     1621 ggaggttcca acccgaaatt tgagaacatt gcagaaggtt taagagctct cctggctagg
     1681 agtcacgtag aaaggactac cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga
     1741 ggtagtaaga cctcccttta caacctaagg cgaggaactg cccttgctat tccacaatgt
     1801 cgtcttacac cattgagtcg tctccccttt ggaatggccc ctggacccgg cccacaacct
     1861 ggcccgctaa gggagtccat tgtctgttat ttcatggtct ttttacaaac tcatatattt
     1921 gctgaggttt tgaaggatgc gattaaggac cttgttatga caaagcccgc tcctacctgc
     1981 aatatcaggg tgactgtgtg cagctttgac gatggagtag atttgcctcc ctggtttcca
     2041 cctatggtgg aaggggctgc cgcggagggt gatgacggag atgacggaga tgaaggaggt
     2101 gatggagatg agggtgagga agggcaggag tgatgtaact tgttaggaga cgccctcaat
     2161 cgtattaaaa gccgtgtatt cccccgcact aaagaataaa tccccagtag acatcatgcg
     2221 tgctgttggt gtatttctgg ccatctgtct tgtcaccatt ttcgtcctcc caacatgggg
     2281 caattgggca tacccatgtt gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt
     2341 ggaaaacatt agcgacattt acctggtgag caatcagaca tgcgacggct ttagcctggc
     2401 ctccttaaat tcacctaaga atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt
     2461 cacgccccct tgggaggtgg cggcatatgc aaaggatagc actcccactc tactactggg
     2521 tatcatatgc tgactgtata tgcatgagga tagcatatgc tacccggata cagattagga
     2581 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga
     2641 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga
     2701 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga
     2761 tagcatatgc tacccagata tagattagga tagcatatgc tatccagata tttgggtagt
     2821 atatgctacc cagatataaa ttaggatagc atatactacc ctaatctcta ttaggatagc
     2881 atatgctacc cggatacaga ttaggatagc atatactacc cagatataga ttaggatagc
     2941 atatgctacc cagatataga ttaggatagc ctatgctacc cagatataaa ttaggatagc
     3001 atatactacc cagatataga ttaggatagc atatgctacc cagatataga ttaggatagc
     3061 ctatgctacc cagatataga ttaggatagc atatgctatc cagatatttg ggtagtatat
     3121 gctacccatg gcaacattag cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa
     3181 caaccctgtg cttggcgctc aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa
     3241 tcgcgcccct atcttggccc gcccacctac ttatgcaggt attccccggg gtgccattag
     3301 tggttttgtg ggcaagtggt ttgaccgcag tggttagcgg ggttacaatc agccaagtta
     3361 ttacaccctt attttacagt ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc
     3421 ccactccaca atttcaaaaa aaagagtggc cacttgtctt tgtttatggg ccccattggc
     3481 gtggagcccc gtttaatttt cgggggtgtt agagacaacc agtggagtcc gctgctgtcg
     3541 gcgtccactc tctttcccct tgttacaaat agagtgtaac aacatggttc acctgtcttg
     3601 gtccctgcct gggacacatc ttaataaccc cagtatcata ttgcactagg attatgtgtt
     3661 gcccatagcc ataaattcgt gtgagatgga catccagtct ttacggcttg tccccacccc
     3721 atggatttct attgttaaag atattcagaa tgtttcattc ctacactagt atttattgcc
     3781 caaggggttt gtgagggtta tattggtgtc atagcacaat gccaccactg aaccccccgt
     3841 ccaaatttta ttctgggggc gtcacctgaa accttgtttt cgagcacctc acatacacct
     3901 tactgttcac aactcagcag ttattctatt agctaaacga aggagaatga agaagcaggc
     3961 gaagattcag gagagttcac tgcccgctcc ttgatcttca gccactgccc ttgtgactaa
     4021 aatggttcac taccctcgtg gaatcctgac cccatgtaaa taaaaccgtg acagctcatg
     4081 gggtgggaga tatcgctgtt ccttaggacc cttttactaa ccctaattcg atagcatatg
     4141 cttcccgttg ggtaacatat gctattgaat tagggttagt ctggatagta tatactacta
     4201 cccgggaagc atatgctacc cgtttagggt taacaagggg gccttataaa cactattgct
     4261 aatgccctct tgagggtccg cttatcggta gctacacagg cccctctgat tgacgttggt
     4321 gtagcctccc gtagtcttcc tgggcccctg ggaggtacat gtcccccagc attggtgtaa
     4381 gagcttcagc caagagttac acataaaggc aatgttgtgt tgcagtccac agactgcaaa
     4441 gtctgctcca ggatgaaagc cactcagtgt tggcaaatgt gcacatccat ttataaggat
     4501 gtcaactaca gtcagagaac ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca
     4561 gggcccagtt ggcaagttgt accaaccaac tgaagggatt acatgcactg ccccgcgaag
     4621 aaggggcaga gatgtcgtag tcaggtttag ttcgtccggg gcggggatct ggccgcaaat
     4681 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     4741 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4801 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4861 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4921 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4981 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5041 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5101 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5161 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5221 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5281 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5341 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5401 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5461 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5521 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5581 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5641 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     5701 tgccaccagc tttgctctta ggagtttcct aatacatccc aaactcaaat atataaagca
     5761 tttgacttgt tctatgccct agggggcggg gggaagctaa gccagctttt tttaacattt
     5821 aaaatgttaa ttccatttta aatgcacaga tgtttttatt tcataagggt ttcaatgtgc
     5881 atgaatgctg caatattcct gttaccaaag ctagtataaa taaaaataga taaacgtgga
     5941 aattacttag agtttctgtc attaacgttt ccttcctcag ttgacaacat aaatgcgctg
     6001 ctgagaagcc agtttgcatc tgtcaggatc aatttcccat tatgccagtc atattaatta
     6061 ctagtcaatt agttgatttt tatttttgac atatacatgt gaaagacccc acctgtaggt
     6121 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa
     6181 tagaaaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga
     6241 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat
     6301 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     6361 atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag
     6421 ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt
     6481 ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca
     6541 ctcggcgcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc
     6601 ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg
     6661 attgactacc cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc
     6721 tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct
     6781 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
     6841 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
     6901 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa
     6961 atcccgatcg ttttggactc tttggtgcac cccccttaga ggagggatat gtggttctgg
     7021 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
     7081 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
     7141 ctgtgtttct gtatttgtct gaaaatttgg gcccgggcca gactgttacc actcccttaa
     7201 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagttg
     7261 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     7321 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     7381 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     7441 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     7501 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     7561 tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg
     7621 gggcaccccc gccccttgta aacttccctg accctgactt gacaagagtt actaacagcc
     7681 cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc
     7741 tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg
     7801 gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac
     7861 cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga
     7921 tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgccgg
     7981 atcccagtgt ggtggtacgg gaatcacaag tttgtacaaa aaagcaggct tcgccaccat
     8041 ggattacaag gacgacgatg acaagatggg ccagactggg aagaaatctg agaagggacc
     8101 agtttgttgg cggaagcgtg taaaatcaga gtacatgcga ctgagacagc tcaagaggtt
     8161 cagacgagct gatgaagtaa agagtatgtt tagttccaat cgtcagaaaa ttttggaaag
     8221 aacggaaatc ttaaaccaag aatggaaaca gcgaaggata cagcctgtgc acatcctgac
     8281 ttctgtgagc tcattgcgcg ggactaggga gtgttcggtg accagtgact tggattttcc
     8341 aacacaagtc atcccattaa agactctgaa tgcagttgct tcagtaccca taatgtattc
     8401 ttggtctccc ctacagcaga attttatggt ggaagatgaa actgttttac ataacattcc
     8461 ttatatggga gatgaagttt tagatcagga tggtactttc attgaagaac taataaaaaa
     8521 ttatgatggg aaagtacacg gggatagaga atgtgggttt ataaatgatg aaatttttgt
     8581 ggagttggtg aatgcccttg gtcaatataa tgatgatgac gatgatgatg atggagacga
     8641 tcctgaagaa agagaagaaa agcagaaaga tctggaggat caccgagatg ataaagaaag
     8701 ccgcccacct cggaaatttc cttctgataa aatttttgaa gccatttcct caatgtttcc
     8761 agataagggc acagcagaag aactaaagga aaaatataaa gaactcaccg aacagcagct
     8821 cccaggcgca cttcctcctg aatgtacccc caacatagat ggaccaaatg ctaaatctgt
     8881 tcagagagag caaagcttac actcctttca tacgcttttc tgtaggcgat gttttaaata
     8941 tgactgcttc ctacatcgta agtgcaatta ttcttttcat gcaacaccca acacttataa
     9001 gcggaagaac acagaaacag ctctagacaa caaaccttgt ggaccacagt gttaccagca
     9061 tttggaggga gcaaaggagt ttgctgctgc tctcaccgct gagcggataa agaccccacc
     9121 aaaacgtcca ggaggccgca gaagaggacg gcttcccaat aacagtagca ggcccagcac
     9181 ccccaccatt aatgtgctgg aatcaaagga tacagacagt gatagggaag cagggactga
     9241 aacgggggga gagaacaatg ataaagaaga agaagagaag aaagatgaaa cttcgagctc
     9301 ctctgaagca aattctcggt gtcaaacacc aataaagatg aagccaaata ttgaacctcc
     9361 tgagaatgtg gagtggagtg gtgctgaagc ctcaatgttt agagtcctca ttggcactta
     9421 ctatgacaat ttctgtgcca ttgctaggtt aattgggacc aaaacatgta gacaggtgta
     9481 tgagtttaga gtcaaagaat ctagcatcat agctccagct cccgctgagg atgtggatac
     9541 tcctccaagg aaaaagaaga ggaaacaccg gttgtgggct gcacactgca gaaagataca
     9601 gctgaaaaag gacggctcct ctaaccatgt ttacaactat caaccctgtg atcatccacg
     9661 gcagccttgt gacagttcgt gcccttgtgt gatagcacaa aatttttgtg aaaagttttg
     9721 tcaatgtagt tcagagtgtc aaaaccgctt tccgggatgc cgctgcaaag cacagtgcaa
     9781 caccaagcag tgcccgtgct acctggctgt ccgagagtgt gaccctgacc tctgtcttac
     9841 ttgtggagcc gctgaccatt gggacagtaa aaatgtgtcc tgcaagaact gcagtattca
     9901 gcggggctcc aaaaagcatc tattgctggc accatctgac gtggcaggct gggggatttt
     9961 tatcaaagat cctgtgcaga aaaatgaatt catctcagaa tactgtggag agattatttc
    10021 tcaagatgaa gctgacagaa gagggaaagt gtatgataaa tacatgtgca gctttctgtt
    10081 caacttgaac aatgattttg tggtggatgc aacccgcaag ggtaacaaaa ttcgttttgc
    10141 aaatcattcg gtaaatccaa actgctatgc aaaagttatg atggttaacg gtgatcacag
    10201 gataggtatt tttgccaaga gagccatcca gactggcgaa gagctgtttt ttgattacag
    10261 atacagccag gctgatgccc tgaagtatgt cggcatcgaa agagaaatgg aaatcccgga
    10321 tccacgaaat gaaatgggtg cttcaggaga catgagggct gccaaccttt ggccaagccc
    10381 tcttgtgatt aagcacacta agaagaatag ccctgccttg tccttgacag ctgaccagat
    10441 ggtcagtgcc ttgttggatg ctgaaccgcc catgatctat tctgaatatg atccttctag
    10501 acccttcagt gaagcctcaa tgatgggctt attgaccaac ctagcagata gggagctggt
    10561 tcatatgatc aactgggcaa agagagtgcc aggctttggg gacttgaatc tccatgatca
    10621 ggtccacctt ctcgagtgtg cctggctgga gattctgatg attggtctcg tctggcgctc
    10681 catggaacac ccggggaagc tcctgtttgc tcctaacttg ctcctggaca ggaatcaagg
    10741 taaatgtgtg gaaggcatgg tggagatctt tgacatgttg cttgctacgt caagtcggtt
    10801 ccgcatgatg aacctgcagg gtgaagagtt tgtgtgcctc aaatccatca ttttgcttaa
    10861 ttccggagtg tacacgtttc tgtccagcac cttgaagtct ctggaagaga aggaccacat
    10921 ccaccgtgtc ctggacaaga tcacagacac tttgatccac ctgatggcca aagctggcct
    10981 gactctgcag cagcagcatc gccgcctagc tcagctcctt ctcattcttt cccatatccg
    11041 gcatatgagt aacaaacgca tggagcatct ctacaacatg aaatgcaaga acgtggtacc
    11101 cctctatgac ctgctcctgg agatgttgga tgcccaccgc cttcatgccc cagccagtcg
    11161 catgggagtg cccccagagg agcccagcca gacccagctg gccaccacca gctccacttc
    11221 agcacattcc ttacaaacct actacatacc cccggaagca gagggcttcc ccaacacgat
    11281 ctaggaccca gctttcttgt acaaagtggt gatattccag ctgagcgccg gtcgctacca
    11341 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt
    11401 tcgcgtaagg aaatccatta tgtactattt tcgagcggcc gccagcacag tggtcgacga
    11461 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
    11521 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
    11581 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca
    11641 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg
    11701 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
    11761 cagatggtcc ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc
    11821 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
    11881 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
    11941 tcactcgggg cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa
    12001 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
    12061 gtgattgact acccgtcagc gggggtcttt cacatgcagc atgtatcaaa attaatttgg
    12121 ttttttttct taagtattta cattaaatgg ccatagttgc attaatgaat cggccaacgc
    12181 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    12241 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    12301 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    12361 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12421 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12481 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12541 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12601 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12661 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12721 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12781 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12841 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12901 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12961 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    13021 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    13081 tagatccttt tgcggccgaa ttagatccag acatgataag atacattgat gagtttggac
    13141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
    13201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
    13261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
    13321 aatgtggtat ggctgattat gatcctctag agtcggtggg cctcgggggc gggtgcgggg
    13381 tcggcggggc cgccccgggt ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt
    13441 cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt
    13501 gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa
    13561 ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc gctcggccgc
    13621 ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt cgggcgagac
    13681 gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca ggaggccttc
    13741 catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg ggccgatctc
    13801 ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc
    13861 gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga agagttcttg
    13921 cagctcggtg acccgctcga tgtggcggtc cggg
//
