LOCUS       sequence-192087-        3723 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea Runt-1 
            (also known as runt).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG94-runt-1
FEATURES             Location/Qualifiers
     source          1..3723
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..600
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     501..520
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     754..771
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(783..830)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        844..862
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        866..884
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     866..883
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1384..1402)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1406..1424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1438..1485
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             1892..2686
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1946..1965)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2556..2575
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2704..2772,2773..3564)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2922..2941)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
       61 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
      121 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
      181 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
      241 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      301 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      361 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      421 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      481 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      541 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      601 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      661 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
      721 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcacgc
      781 gtcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag gcgcgccgga
      841 tcctaatacg actcactata gggagattta ggtgacacta tagaagtgac cttagtcgca
      901 gtggaagagg aaaatatttc gatgtttata tccacattga gtgcaacccg aaaatcatcg
      961 ccgtttacac aaacgctatc aaggttacag tcgacggtcc tagagagccc agaaacaaac
     1021 acaaaaactt ttacttcaca aagaacaaca taaaaattcc caacttcatt ccaaaatcgg
     1081 aacccgactc gcatttctgt tacagcggct ctcctgggct cactgatcct ccgagctatt
     1141 ggagttcaac aaaaaccgat acgaaaaatc tttcagaaaa tgattcattt gaacagaaat
     1201 caaacttgat aatctctccg tctcttcttt atccatatgg gacacccaac ggttgccatt
     1261 tcagtttacc tcagaccaag caggacaatt cttcaatgct acaattcatg ctgatggcaa
     1321 cgacacttcg aatcgggctt cctttggcag agatctacaa ctcgcaatcc catcaaaggt
     1381 ctccctttag tgagggttaa ttctccctat agtgagtcgt attaggatcc ggcgcgccta
     1441 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgacgcg tcgatttcgg
     1501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaattc
     1561 agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg
     1621 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
     1681 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
     1741 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
     1801 caaagtaaac tggatggctt tcttgccgcc aatgatctga tggcgcaggg gatcaagatc
     1861 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     1921 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     1981 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     2041 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     2101 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     2161 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatcccacc ttgctcctgc
     2221 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     2281 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     2341 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     2401 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     2461 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     2521 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     2581 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     2641 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattg aaaaaggaag
     2701 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2761 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2821 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2881 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2941 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3001 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3061 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3121 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3181 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3241 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3301 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3361 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3421 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3481 tacacgacgg ggagccaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3541 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3601 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3661 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3721 atc
//
