LOCUS       sequence-19209-e         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf132
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             571..612
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             622..639
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ccgaattcca gctgaccacc atgaatgcgt ataataaagc cgattcgttt tctttagagt
       61 ctgattctat caaagatgtt atacacgatt atatttgttg gctcagtatg actgatgaaa
      121 tgagaccatc tatcggaaac gtctttaaag cgatggaaac gtttaagata gacgcggtta
      181 gatattacga tggtaacata tatgaattag ctaaagatat aaatgcgatg tcgtttgacg
      241 gttttataag atctctacaa actatcgctt caaagaaaga taaactcact gtttatggaa
      301 ccatgggact gctgtctatt gtcgtagata ttaacaaagg ttgtgatata tccaatatca
      361 agttcgctgc cggaataatc attttaatgg agtatatttt tgatgacacg gatatgtctc
      421 atcttaaagt agcactctat cgtagaatac agagacgtga tgatgtagat agataagcaa
      481 ttcccgggga tccgaataaa gggtcaagac aattctgcag atatccagca cagtggcggc
      541 cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt
      601 ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca
      661 gcctcgactg tgccttct
//
