LOCUS       sequence-192095-        2140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BB1 with insert: TDH3tt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Prielhofer R, Barrero JJ, Steuer S, Gassler T, Zahrl R, Baumann K, 
            Sauer M, Mattanovich D, Gasser B, Marx H
  TITLE     GoldenPiCS: a Golden Gate-derived modular cloning system for applied
            synthetic biology in the yeast Pichia pastoris.
  JOURNAL   BMC Syst Biol. 2017 Dec 8;11(1):123. doi: 10.1186/s12918-017-0492-3.
  PUBMED    29221460
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Syst 
            Biol."; date: "2017-12-8"; volume: "11(1):123. doi"; pages: " 
            10.1186/s12918-017-0492-3"
COMMENT     Sequence Label: BB1_34_TDH3tt
FEATURES             Location/Qualifiers
     source          1..2140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      138..165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(177..764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(257..276)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     804..821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     805..821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1240..1256)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1240..1256)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      1304..1335
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1336..1438
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             join(1439..2140,1..114)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1512..1531)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
       61 aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgagga
      121 cctaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc
      181 cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga
      241 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      301 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      361 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      421 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      481 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      541 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      601 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg
      661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt
      781 tctaccgaag aaaggcccac ccgtgtaaaa cgacggccag tttatctagt cagcttgatt
      841 ctagctgatc gtggaccgga aggtgagcca gtgagttgat tgcagtccag ttacgctgga
      901 gtctgaggct cgtcctgaat gatatgcggc ctcgaattcg aagacgcgct ttcgatttgt
      961 atgtgaaata gctgaaattc gaaaatttca ttatggctgt atctacttta gcgtattagg
     1021 catttgagca ttggcttgaa caatgcgggc tgtagtgtgt caccaaagaa accattcggg
     1081 ttcggatctg gaagtcctca tcacgtgatg ccgatctcgt gtattttatt ttcagataac
     1141 acctgaggac tttcgctgcg tcttcgaatt cgcgcgtgat cttacggcat tatacgtatg
     1201 atcggtccac gatcagctag attatctagt cagcttgatg tcatagctgt ttcctgaggc
     1261 tcaatactga ccatttaaat catacctgac ctccatagca gaaagtcaaa agcctccgac
     1321 cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata atgaaataag
     1381 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     1441 gagccatatt caacgggaaa cgtcttgctt gaagccgcga ttaaattcca acatggatgc
     1501 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     1561 tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     1621 tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat ttatgcctct
     1681 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     1741 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     1801 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     1861 taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     1921 tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     1981 aatgcataaa ctcttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2041 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2101 aatcgcagac cgataccagg atcttgccat cctatggaac
//
