LOCUS       sequence-192099-        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG1013-LDLRR-1
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     505..524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     758..775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(787..834)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        848..866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        870..888
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     870..887
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(2099..2117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(2121..2139)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2153..2200
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2607..3401
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2661..2680)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3271..3290
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3419..3487,3488..4279)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3637..3656)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
       61 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
      121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
      181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
      241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
      301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
      361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
      421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
      481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
      541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
      601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
      661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
      721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      781 acgcgtcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctaggcgcgc
      841 cggatcctaa tacgactcac tatagggaga tttaggtgac actatagaag tgacctttta
      901 attgttttta agaataactt gaaaatcgtt tgagaatgat ctttgacgag aactaagagc
      961 ttcgcaattg tatatcccat cgtctccggc cttgaaatcc tttattgtca gtgaacttgt
     1021 aacgctatta caatcggtag aaaagaagtt ggttgaaaaa cgtgtggaat tttgaatgca
     1081 accccaatta aatctccaaa caagaactgg caatggctta cccgtagctt cacaggttag
     1141 agttaccagt tgcgattttg tcgccacaac ttttgttgcc gatgtttttg tgatttgtgg
     1201 agaagtacaa ccggtttcgt cctccccttg agaacagtct atgttttcat cacagacgta
     1261 gccacgaggt attgttttgt tatcagaaca agtgaagaac ttacccccac agttattcaa
     1321 agaaaagcca tcgcaatttt gttcatcgta gccatttcca cagtcttctt ctccatcaca
     1381 aatccaatga attaaaaagt aatttttctt tggtaagact tgattattta tttcacattt
     1441 tctggatatt gggtcacact ctctgcacgt aattggatct tcatcagatt tatcagcgca
     1501 gtgatttatt ttatcacaaa attttgcatt ttctaaacat tcaccatttt gacaaaaccg
     1561 atagttttat tttgttgatt tagccatgtg tatgtaaagt catccacgat cgtttcctgt
     1621 ggcagaccca agtccgagca ttgtctttta atctgtccac cgattttcaa agattcttca
     1681 ggaagaatgt caattgaaat tgaaggaatt gtaattttca aatgaagcgt cctattgaat
     1741 ctaatttgag ggtctttcac tttttcaact gaacaattat aaatcccttc gtcttgtttt
     1801 aagagagatt ttatgtaaag atatctattt gcagctgcta cctttccatc tttatccagc
     1861 catcttattg aagtttcatt aagtgcataa tttgtaacat tgcaagcaat atttataaat
     1921 tgcatttcag atttgacaat tgaatcattt tttaatggaa aaaatgtgat atttggagga
     1981 gaagtctctt cttcttcaga tgcacctcca aaagtgaatt cctcagtttc ggctttaact
     2041 aaactttgat aaatgcaaat taatgcaaaa atcttacaaa ttgaaatcat aaggtctccc
     2101 tttagtgagg gttaattctc cctatagtga gtcgtattag gatccggcgc gcctagcata
     2161 accccttggg gcctctaaac gggtcttgag gggttttttg acgcgtcgat ttcggcctat
     2221 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aattcagggc
     2281 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
     2341 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     2401 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     2461 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     2521 taaactggat ggctttcttg ccgccaatga tctgatggcg caggggatca agatctgatc
     2581 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     2641 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     2701 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     2761 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     2821 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     2881 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc ccaccttgct cctgccgaga
     2941 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     3001 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     3061 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     3121 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     3181 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     3241 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     3301 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     3361 agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattgaaaaa ggaagagtat
     3421 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     3481 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3541 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3601 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3661 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3721 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3781 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3841 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3901 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3961 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     4021 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     4081 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     4141 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     4201 gacggggagc caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     4261 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     4321 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4381 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaa
//
