LOCUS       sequence-192132-        5986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea LDLRR-3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG1014-LDLRR-3
FEATURES             Location/Qualifiers
     source          1..5986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      22..610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     511..530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     764..781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(793..840)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        854..872
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        876..894
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     876..893
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(3657..3675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(3679..3697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      3711..3758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             4165..4959
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4219..4238)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4829..4848
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(4977..5045,5046..5837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5195..5214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
       61 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
      121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
      181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
      241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
      301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
      361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
      421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      781 ggaagcacgc gtcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
      841 gcgcgccgga tcctaatacg actcactata gggagattta ggtgacacta tagaagtgac
      901 cttatgaagt atctatcgtt gtttttcgct attctgtcgt ttctatatgt tgttaatgct
      961 caagaagagg ccatattcgt tgaacttggc catacagtta ttctgcaatg caggccacaa
     1021 tcggtgatgt caaacgttcc aacaacatac gaatggatag atcctagaaa caatctacgg
     1081 ggatcaaacg tcaatttaac tataacaaat ttttcctatg aacatgtagg cagatatacg
     1141 tgcaaagcta gccaaatcat tgacggtagg ctctcaacaa ctaacaaatc gctccgacta
     1201 caagtcaaag ataagactgg tctaggttcg aataatttcc aagtgattgt tcctcaagtt
     1261 tacgaaacat tcgcaagtgg gataatattt atggcatgta aagaggcatc cggtgcgtca
     1321 gcaaattata aatatgaatg gatcactcca agtggagaaa gacaaagttc atcgtcgttt
     1381 tttaaaaacc aagtcggtcc ggaattcacg ggccaataca cttgtagaat gactcgaatc
     1441 tcggataatc aagtgtctga aggtaaagcc tttgtacaag ttaaagcact tcctatcatg
     1501 attgaaaagg tatctggaaa ccctgtgttt ggtgaaacgg ttgaactcaa atgccaagtt
     1561 gataaatcaa agacggacaa taaagtattc tcgtcgtaca cgtattcttg gttggattcg
     1621 aaaaataata tcatttcgaa tgatcaaatt ttgagattga aactcgacga ccaaaccaag
     1681 tttggaacct atacttgtag aataaatact cagtccatga atcctccata cgttcagaac
     1741 caagtaatcg aaatcagatt taccggtgat tcaaacactg gaagtagatc aagaaatgaa
     1801 tattcaaccc gttttagcag caatccgaaa aatgttcggt tcttcgaacc gttagaactc
     1861 aactgcgaag taactccacc attgagagac gtgacctatg aaatctactt caatgacaga
     1921 ctaatttccc agtcgagcag gcacaccatt ccaaggctgg acaaaacaaa tatgggagtt
     1981 tatcaatgcg tttctagagg aagagattca aacactaacg agctgataaa ctacaattct
     2041 acatacgtta ttcaagcaga attcaaatct gcatctaaaa tcgaattatc agaaccaccg
     2101 ggacctcaga tctttgttcg agcaggagat aaaagagaat tgcactgtga aattggtgta
     2161 aataccggct tgcaaggtaa aaatgccatc tggtacttta aaggtcaaga aataaactcg
     2221 aacgattatg cgagatataa cattcaagtg attccaagac tctatgtgag catcatggta
     2281 ctcagaaata ttcaacaaac caatgaagga aaatacgaat gcagggtccg aggcgaaacc
     2341 aaagaggctt acatcattgt aatggctaaa agcaaaaata cagaagttcc tccaagtcaa
     2401 caaactcgaa aagttggcga tggaatacct gtagaattcc attgtagagt cgaaggatat
     2461 caaactagaa atcgggatgt tatatggtca tttttgagca aagagacagg acaaataact
     2521 aatatagatt caagatggag tcgatctgat gattcagcag ccgaagaaac gagtttcatt
     2581 attctcaccc aagcagaaat gaaagatgca ggagtgtata tttgtacgga acctcaaacc
     2641 cgagatacgg ctagagccac tttaattgtt gaaagtgacc tgagaatcat tgtaacacct
     2701 ccaatcatca aactcagatc tgggcaaaca gcaacattcc agtgctatgt tttaggtgca
     2761 ggaggaagtc cagactatcc agaatttttc atggctaatg gcggtcgagc agcggacact
     2821 caaagaacaa ctgttgaaaa gttggaagca ggaaaagtgc aacttaccta tcgggcagtg
     2881 actacaaatg acaacggcac ccgattcact cgccgaaaag gaaggtcaac ggctgaagtt
     2941 ttagtcttgg ttgaccctgt ctgctcatct ggatcaagat tatgtcgaaa tggtgaatgc
     3001 ataaattcag caaaattttg tgacggaaaa ccagactgtg cagatggatc cgatgaagat
     3061 caagaatttt gccgccaatg tgatccgctt tcaaagcgtt gcgaaaccta taatggcgtc
     3121 gcacctataa atccaattta tcaagcacac tgggcctgtg atggtgaaga cgactgcggc
     3181 aacggatacg atgaagctaa atgttttggt aatgttcttg aatgtggagg tttgaagtac
     3241 caatgtaatg atggaaccaa atccattcca agagcttttg tttgcgataa agacactgac
     3301 tgtgtcaatg gagatgatga aagccgatgc atccaaccaa taataataca agaatcatta
     3361 gattcataca agatcaatgc tcgaaggggg gaaactgtta ccctcacatg tcaggttact
     3421 ggatttccat tgccaaaaat catttggcga ttcaattggg gctgtttgac gaatgagcgc
     3481 cgaatggtcg tcatagaatc atatagaaat tgtggtgcca cagttagcac cttgactatc
     3541 tcagaattcc aaccgggaga tgatggcatt tacaattgcg aagctctgtc aggaggaaag
     3601 agagcctggt ccaaagatta tcaactgttg atgaattttc gaaattaaaa ggtctccctt
     3661 tagtgagggt taattctccc tatagtgagt cgtattagga tccggcgcgc ctagcataac
     3721 cccttggggc ctctaaacgg gtcttgaggg gttttttgac gcgtcgattt cggcctattg
     3781 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa ttcagggcgc
     3841 aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc
     3901 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag
     3961 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca
     4021 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
     4081 aactggatgg ctttcttgcc gccaatgatc tgatggcgca ggggatcaag atctgatcaa
     4141 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     4201 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     4261 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     4321 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     4381 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     4441 ctattgggcg aagtgccggg gcaggatctc ctgtcatccc accttgctcc tgccgagaaa
     4501 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     4561 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     4621 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     4681 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     4741 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     4801 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     4861 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     4921 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttgaaaaagg aagagtatga
     4981 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5041 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5101 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5161 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5221 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5281 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5341 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5401 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5461 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5521 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5581 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5641 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5701 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5761 cggggagcca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5821 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgt
//
