LOCUS       sequence-19215-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf138
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             479..520
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             530..547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(570..587)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    576..687
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtacccttca ccgaattcca gctgaccacc atgaggactc tacttattag atatattctt
       61 tggagaaatg acaacgatca aacctattat aatgatgatt ttaaaaagct tatgttgttg
      121 gatgaattgg tagatgacgg cgatgtatgt acattgatta agaacatgag aatgacgctg
      181 tccgacggtc cattgctaga tagattgaat caaccagtta ataatataga agacgctaag
      241 cgaatgatcg ctattagtgc caaagtggct agagacattg gtgaacgttc agaaattaga
      301 tgggaagagt cattcaccat actctttagg atgattgaaa catattttga tgatctaatg
      361 attgatctat atggtgaaaa ataagcaatt cccggggatc cgaataaagg gtcaagacaa
      421 ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgc ggttcgaagg
      481 taagcctatc cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca
      541 tcaccattga gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
      601 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
      661 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtc
//
