LOCUS       sequence-192162-        3865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dsRNA and riboprobe synthesis for Schmidtea mediterranea estrella.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Roberts-Galbraith RH, Brubacher JL, Newmark PA
  TITLE     A functional genomics screen in planarians reveals regulators of 
            whole-brain regeneration.
  JOURNAL   Elife. 2016 Sep 9;5. pii: e17002. doi: 10.7554/eLife.17002.
  PUBMED    27612384
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-09-9"; volume: "5. pii: e17002. doi"; pages: " 
            10.7554/eLife.17002"
COMMENT     Sequence Label: pRRG820-estrella
FEATURES             Location/Qualifiers
     source          1..3865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      8..596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     497..516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     750..767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(779..826)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        840..858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        862..880
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     862..879
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1522..1540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1544..1562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1576..1623
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             2030..2824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2084..2103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2694..2713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(2842..2910,2911..3702)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3060..3079)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
       61 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      121 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
      361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
      421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
      481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
      541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
      601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      721 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcacgcgtca
      781 aaaaacccct caagacccgt ttagaggccc caaggggtta tgctaggcgc gccggatcct
      841 aatacgactc actataggga gatttaggtg acactataga agtgaccttg aagtcaatgg
      901 aacagacgag aattctagct gaatgttttc tctacaattt agtaatagga atagagaatt
      961 gagaaacttc ggagtaagca tcgtttagca atagttttgt aacgacattt tcaccgtaag
     1021 ttaaacattc gtatcggtac gaaccgtaat aatatcgatt ttcgcagcgg ttggtttgtg
     1081 tcatgttttg aggtatttga caagcgagtt catctttatc aatcttttta agccgtttta
     1141 cacctacaga ttcgataggc accttatcct tacacaagta ttgtaacact ttctcgtaga
     1201 tgtcagattg tagactcaaa ctattcgaac taaagttttt cattatttca acgctcacgt
     1261 catatgccca cttttccgca taatcacttg acacagtaca tctgctgctt tggcccatat
     1321 cacaaccaga ttcattaaac atctttaccg tttgaatatc cttaattttt attacaacct
     1381 cttaaatgtt attcgttttt ctgacgtttc tcgcaaattc aatgacgaat tcaaagcttt
     1441 gaaacgattc attcagcaaa cggtttttcg tttcaacaca taaacctgta taattctggt
     1501 cgctcactaa ttcaaggtct ccctttagtg agggttaatt ctccctatag tgagtcgtat
     1561 taggatccgg cgcgcctagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     1621 ttgacgcgtc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     1681 cgaattttaa caaaattcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc
     1741 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag
     1801 ggaaaacgca agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct
     1861 agactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
     1921 taaggttggg aagccctgca aagtaaactg gatggctttc ttgccgccaa tgatctgatg
     1981 gcgcagggga tcaagatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     2041 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     2101 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     2161 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     2221 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     2281 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     2341 atcccacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     2401 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     2461 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     2521 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     2581 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     2641 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     2701 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     2761 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     2821 ctgaattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2881 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2941 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3001 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3061 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3121 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3181 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3241 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3301 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3361 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3421 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3481 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3541 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3601 tcccgtatcg tagttatcta cacgacgggg agccaggcaa ctatggatga acgaaataga
     3661 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3721 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3781 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3841 tcagaccccg tagaaaagat caaag
//
