LOCUS       sequence-192164-        3384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle yeast Ckb1 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Ckb1
FEATURES             Location/Qualifiers
     source          1..3384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(973..1072)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1089..1108)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1090..1108)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1113..1129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1113..1129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1231..1250)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1242..2051
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1309..1328)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2198..2786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2687..2706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2940..2957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3116..3143)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3235..3321)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3370..3384,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3384,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg tcgcaagagt ttgtggaaga ctattcacgt acagggtcct
      181 cagacgacga ggatagtgga gcttatgacg agtggattcc atcgttctgt tccagatttg
      241 gtcacgagta cttctgccaa gtcccgacag agtttatcga ggatgatttc aatatgacct
      301 cgttatccca agaggtaccc cactaccgga aagctctgga tttgatattg gatttggagg
      361 ctatgagtga tgaagaagag gacgaagacg acgtggtgga agaagacgaa gtggatcaag
      421 agatgcagag taatgacggt catgatgaag gcaaaaggag aaacaagtcg ccagtggtga
      481 acaagagtat tatcgagcac gcagcagaac agttatacgg attgatacat gcaagattta
      541 ttctgaccaa gccaggcctg caagcaatgg ctgaaaaatt cgaccacaag gaatttggaa
      601 cgtgcccccg ttactattgt aatggcatgc agttgctgcc ttgtgggttg agcgatacag
      661 ttgggaaaca caccgtaagg ttgtactgtc ccagttgtca ggacttgtat cttccccaat
      721 catctagatt cctgtgctta gaaggtgcat tttgggggac aagcttccct ggtgtgttct
      781 tgaaacattt caaagaattg gaagagtatg tggaaaggaa aagtaaagag tcctacgagt
      841 tgaaagtttt tgggttcagg ataaatgatg aggcagtatc tggtcctaga atgaagtggt
      901 tgagacaata tccatccacg gaggaggact gggaagaatt tgccaaatgc gagttcgaga
      961 caccggcggt taacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
     1021 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1081 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1141 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1201 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1261 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1321 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     1381 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1441 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1501 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     1561 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1621 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     1681 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     1741 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     1801 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1861 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1921 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1981 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     2041 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2101 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2341 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2941 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3001 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3061 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3121 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3181 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3241 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3301 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     3361 ggatgttttc ccagtcacga cgtt
//
