LOCUS       sequence-192165-        3324 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle yeast Ckb2 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Ckb2
FEATURES             Location/Qualifiers
     source          1..3324
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(913..1012)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1029..1048)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1030..1048)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1053..1069)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1053..1069)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1171..1190)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1182..1991
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1249..1268)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2138..2726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2627..2646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2880..2897
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3056..3083)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3175..3261)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3310..3324,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3324,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg ggcagtagat cggagaatgt gggaacagtg actagagaag
      181 gctccagagt ggaacaagat gatgtcctca tggatgatga ctctgactca tcggaatacg
      241 tggatatgtg gatcgattta tttcttggga gaaaaggcca tgaatatttc tgtgatgtcg
      301 atcctgagta catcactgat cgttttaatc tgatgaactt gcaaaagaca gtttcaaaat
      361 tttcctacgt ggtacaatat attgtggatg atttggatga tagtatccta gagaatatga
      421 cacatgcccg tttagaacaa ttagaatcag attcccggaa gctgtatggt ctcatccatg
      481 cacgctacat tataactatc aaaggattgc aaaaaatgta cgctaagtac aaagaggcgg
      541 actttggaag atgtccccgt gtgtattgta atttacaaca gttgttaccg gttggtttgc
      601 atgacattcc tggtatagat tgtgtcaagt tatattgtcc atcttgtgag gatctttata
      661 taccaaaatc atcgagacat agttccattg atggtgcata ctttggaaca agttttccag
      721 gtatgttttt gcaagcattt ccagatatgg tacccaaaca cccaactaaa agatatgttc
      781 caaaaatatt tggatttgaa ttacataaac aagctcaact gacaaggtgg caagaactac
      841 aacgactcaa gttagttgag aaacttgaat ccaaagatgt tgatttgacg aaaagtggtg
      901 gttttaaaac caacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat
      961 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag
     1021 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc
     1081 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
     1141 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
     1201 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
     1261 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
     1321 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
     1381 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
     1441 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
     1501 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
     1561 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
     1621 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
     1681 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
     1741 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
     1801 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
     1861 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
     1921 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
     1981 agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt
     2041 gacgggacgg cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact
     2101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2281 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2881 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2941 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3001 agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa acgcaaaaag
     3061 gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg gcgggcgtcc
     3121 tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg gcggatttgt
     3181 cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtcttccga
     3241 ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta acgctagcat
     3301 ggatgttttc ccagtcacga cgtt
//
