LOCUS       sequence-192168-        3192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle yeast Utp16 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Utp16
FEATURES             Location/Qualifiers
     source          1..3192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(781..880)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(897..916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(898..916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(921..937)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(921..937)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1039..1058)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1050..1859
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1117..1136)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2006..2594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2495..2514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2748..2765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2924..2951)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3043..3129)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(3178..3192,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3192,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg agtaatggac acgtcaagtt tgatgccgat gaaagtcaag
      181 ccagcgcaag tgctgtcacg gatagacaag atgatgtgct ggtaatatca aaaaaggaca
      241 aggaagtgca ttcaagtagt gatgaggaaa gtgatgatga tgatgctcca caagaagaag
      301 ggcttcactc tgggaaaagt gaggtagaat cacagatcac tcagagggag gaggctataa
      361 ggctggaaca atcacaattg cggtccaaga gaaggaagca aaatgaatta tacgccaagc
      421 aaaagaaatc agtgaatgag acagaggtca ctgatgaagt aattgcggag cttcctgagg
      481 aattgctaaa aaatatagat cagaaggacg aaggtagtac gcagtactca tcttcaagac
      541 atgtaacttt cgataaattg gatgagtcag atgaaaatga agaggcattg gctaaagcta
      601 taaaaactaa gaaaaggaag acattaaaaa atttaaggaa agattcggtg aaaagaggaa
      661 aatttagagt tcaattgtta tccaccacac aagattctaa aacattacca ccaaagaaag
      721 aatcatccat catacgttca aaagaccgtt ggctaaatag aaaggctttg aataaaggga
      781 acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
      841 acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct
      901 atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa
      961 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
     1021 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc
     1081 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
     1141 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt
     1201 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1261 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1321 atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat
     1381 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1441 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1501 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1561 ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag
     1621 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1681 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1741 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1801 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1861 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1921 caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc
     1981 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2041 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2101 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     2161 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2221 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     2281 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     2341 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     2401 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     2461 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     2521 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     2581 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     2641 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     2701 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2761 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2821 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2881 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag
     2941 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc
     3001 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag
     3061 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg
     3121 ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc
     3181 agtcacgacg tt
//
