LOCUS       sequence-192174-       11977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing 
            dCas9-Mxi1 and AvrII site for sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Schwartz C, Frogue K, Ramesh A, Misa J, Wheeldon I
  TITLE     CRISPRi repression of nonhomologous end-joining for enhanced genome 
            engineering via homologous recombination in Yarrowia lipolytica.
  JOURNAL   Biotechnol Bioeng. 2017 Aug 19. doi: 10.1002/bit.26404.
  PUBMED    28832943
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2017 Aug 19. doi: 10.1002/bit.26404."
COMMENT     Sequence Label: pCRISPRi_Mxi1_yl
FEATURES             Location/Qualifiers
     source          1..11977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(1740..1766,1767..1769,1770..4256,4257..4259,
                     4260..5843)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5856..5876
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      6058..6305
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6074..6092)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6324..6340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6324..6340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6337..6359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6348..6364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6372..6378,6379..6396,6397..6402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6417..6438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6555..6572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6726..7314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6806..6825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7485..8276,8277..8345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8108..8127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8346..8450)
                     /gene="bla"
                     /label=AmpR promoter
     misc_RNA        8926..9001
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     9051..9069
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt
      421 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca
      481 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat
      541 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat
      601 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggggatccc ccgggttcga
      661 aggtaccaag gaagcatgcg gtacccgaat tcctgaggtg tctcacaagt gccgtgcagt
      721 cccgccccca cttgcttctc tttgtgtgta gtgtacgtac attatcgaga ccgttgttcc
      781 cgcccacctc gatccggtct agactgaggt gtctcacaag tgccgtgcag tcccgccccc
      841 acttgcttct ctttgtgtgt agtgtacgta cattatcgag accgttgttc ccgcccacct
      901 cgatccgggg atccctgagg tgtctcacaa gtgccgtgca gtcccgcccc cacttgcttc
      961 tctttgtgtg tagtgtacgt acattatcga gaccgttgtt cccgcccacc tcgatccggg
     1021 tcgacctgag gtgtctcaca agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt
     1081 gtagtgtacg tacattatcg agaccgttgt tcccgcccac ctcgatccgg gagctcctga
     1141 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
     1201 gtacattatc gagaccgttg ttcccgccca cctcgatccg gtctagactg aggtgtctca
     1261 caagtgccgt gcagtcccgc ccccacttgc ttctctttgt gtgtagtgta cgtacattat
     1321 cgagaccgtt gttcccgccc acctcgatcc ggggatccct gaggtgtctc acaagtgccg
     1381 tgcagtcccg cccccacttg cttctctttg tgtgtagtgt acgtacatta tcgagaccgt
     1441 tgttcccgcc cacctcgatc cgggtcgacc tgaggtgtct cacaagtgcc gtgcagtccc
     1501 gcccccactt gcttctcttt gtgtgtagtg tacgtacatt atcgagaccg ttgttcccgc
     1561 ccacctcgat ccgggcatgc ctgcagaagc ttttgtggtt gggactttag ccaagggtat
     1621 aaaagaccac cgtccccgaa ttacctttcc tcttcttttc tctctctcct tgtcaactca
     1681 cacccgaaat cgttaagcat ttccttctga gtataagaat cattcaaagg cgcgccacaa
     1741 tggataagaa atactccatt ggcctggcca tcggaaccaa ctccgtgggt tgggccgtga
     1801 tcaccgatga gtacaaggtg ccctctaaga aattcaaggt cctgggcaac accgaccgac
     1861 actccatcaa gaagaacctg atcggcgctc tgctcttcga ctctggcgag accgctgagg
     1921 ccacccgact gaagcgaacc gctcgaagac gatacacccg aagaaagaac cgaatctgtt
     1981 acctgcagga gatcttctct aacgagatgg ccaaggtgga cgactctttc ttccaccgac
     2041 tggaggagtc tttcctggtg gaggaggaca agaagcacga gcgacacccc atcttcggca
     2101 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgcgaaaga
     2161 agctggtgga ctctaccgac aaggccgacc tgcgactgat ctacctggcc ctggcccaca
     2221 tgatcaagtt ccgaggccac ttcctgatcg agggcgacct gaaccccgac aactctgacg
     2281 tggacaagct gttcatccag ctggtgcaga cctacaacca gctcttcgaa gagaacccca
     2341 ttaacgcttc tggcgtggat gctaaggcca tcctgtctgc ccgactgtct aagtctcgac
     2401 gactcgagaa cctgattgct cagctccccg gagagaagaa gaacggtctg ttcggaaacc
     2461 tgattgctct gtccctgggt ctcaccccta acttcaagtc caacttcgat ctggctgagg
     2521 acgctaagct gcagctgtct aaggacacct acgacgatga cctggataac ctgctcgccc
     2581 agattggcga ccagtacgcc gacctgttcc tggccgccaa gaacctgtct gacgccatcc
     2641 tgctgtctga catcctgcga gtgaacaccg agatcaccaa ggcccccctg tctgcctcca
     2701 tgattaagcg atacgatgag caccaccagg atctgaccct cctcaaggct ctggtccgac
     2761 agcagctgcc cgagaagtac aaggagattt tcttcgacca gtctaagaac ggctacgccg
     2821 gctacatcga cggcggcgcc tctcaggagg agttctacaa gttcattaag cccatcctgg
     2881 agaagatgga cggaaccgag gaactgctcg tgaagctgaa ccgagaggac ctcctgcgaa
     2941 agcagcgaac cttcgacaac ggctctatcc cccaccagat ccacctgggc gagctgcacg
     3001 ccatcctgcg acgacaggag gacttctacc ccttcctgaa ggacaaccga gagaagatcg
     3061 agaagatcct gaccttccga atcccctact acgtgggacc cctggcccga ggaaactctc
     3121 gattcgcttg gatgacccga aagtctgagg agaccattac cccctggaac ttcgaggagg
     3181 tggtggataa gggcgcctct gctcagtctt tcatcgagcg aatgaccaac ttcgacaaga
     3241 acctccccaa cgagaaggtc ctgcccaagc actctctgct ctacgagtac ttcaccgtct
     3301 acaacgagct caccaaggtc aagtacgtga ccgagggaat gcgaaagccc gctttcctgt
     3361 ctggagagca gaagaaggct attgtggatc tgctcttcaa gactaaccga aaggtcaccg
     3421 tcaagcagct gaaggaggat tacttcaaga agattgagtg tttcgattct gtcgagatct
     3481 ccggcgtcga ggaccgattc aacgcctctc tgggtaccta ccacgacctg ctgaagatta
     3541 tcaaggacaa ggatttcctg gataacgagg agaacgagga tattctcgag gacattgtcc
     3601 tgaccctcac cctgttcgag gatcgagaga tgattgagga gcgactcaag acctacgctc
     3661 acctgttcga cgacaaggtg atgaagcagc tgaagcgacg acgatacacc ggctggggcc
     3721 gactgtctcg aaagctgatc aacggcatcc gagacaagca gtctggcaag accatcctgg
     3781 acttcctgaa gtctgacggc ttcgccaacc gaaacttcat gcagctgatc cacgacgact
     3841 ctctgacctt caaggaggac atccagaagg cccaggtgtc tggccagggc gactctctgc
     3901 acgagcacat cgccaacctg gccggctctc ccgccattaa gaaaggtatc ctgcagaccg
     3961 tcaaggtggt cgatgagctc gtcaaggtga tgggccgaca caagcccgag aacattgtca
     4021 ttgagatggc tcgagagaac cagactactc agaagggtca gaaaaactcc cgagagcgaa
     4081 tgaagcgaat tgaggaaggt attaaggagc tgggatccca gattctcaag gagcatcccg
     4141 tggagaacac tcagctccag aacgagaagc tgtacctgta ctatctgcag aacggtcgag
     4201 acatgtacgt cgaccaggag ctggatatca accgactctc cgactacgat gtggacgcca
     4261 ttgtgcccca gtccttcctg aaggacgatt ctatcgataa caaggtgctg acccgatccg
     4321 acaagaaccg aggcaagtct gacaacgtgc cctccgagga ggtggtcaag aagatgaaga
     4381 actactggcg acagctgctg aacgccaagc tgattaccca gcgaaagttc gacaacctga
     4441 ccaaggccga gcgaggcggc ctgtctgagc tggacaaggc cggcttcatc aagcgacagc
     4501 tggtggagac ccgacagatc accaagcacg tggcccagat cctggactct cgaatgaaca
     4561 ccaagtacga cgagaacgac aagctgatcc gagaggtgaa ggtgatcacc ctgaagtcta
     4621 agctggtgtc tgacttccga aaggacttcc agttctacaa ggtgcgagag attaacaact
     4681 accaccacgc ccacgatgcc tacctgaacg ctgtcgtggg caccgccctc atcaagaagt
     4741 atcccaagct ggagtccgag ttcgtctacg gcgactacaa ggtctacgat gtgcgaaaaa
     4801 tgattgccaa gtccgagcag gagattggca aggctaccgc caagtacttc ttctactcca
     4861 acattatgaa cttcttcaag accgagatta ccctggctaa cggcgagatt cgaaagcgac
     4921 ccctcattga gaccaacgga gagaccggtg agatcgtgtg ggacaaggga cgagacttcg
     4981 ccaccgtgcg aaaggtgctg tctatgcccc aggtgaacat cgtgaagaag accgaggtgc
     5041 agaccggagg tttctctaag gagtccatcc tgcccaagcg aaactctgac aagctgatcg
     5101 cccgaaagaa ggactgggac cccaagaagt acggaggttt cgactctccc accgtggctt
     5161 actctgtgct ggtggtggcc aaggtggaga agggcaagtc taagaagctg aagtctgtga
     5221 aggagctgct gggcattacc atcatggagc gatcttcttt cgagaagaac cccattgact
     5281 tcctggaggc caagggatac aaggaggtga agaaagatct gattatcaag ctccccaagt
     5341 actctctgtt cgagctggag aacggacgaa agcgaatgct ggcctctgcc ggcgagctgc
     5401 agaagggaaa cgagctggcc ctgccctcca agtacgtcaa cttcctgtac ctcgcctccc
     5461 attacgagaa gctgaagggc tctcccgagg ataacgagca gaagcagctc ttcgtggagc
     5521 agcataagca ctacctggac gagatcatcg agcagatctc tgagttctct aagcgagtga
     5581 tcctggctga cgccaacctg gataaggtgc tgtctgctta caacaagcac cgagacaagc
     5641 ccattcgaga gcaggctgag aacatcattc acctgttcac cctgaccaac ctgggagccc
     5701 ccgctgcctt caagtacttc gacaccacca tcgaccgaaa gcgatacacc tctaccaagg
     5761 aggtgctgga cgccaccctg atccaccagt ctatcaccgg cctgtacgag acccgaatcg
     5821 acctgtctca gctgggcggc gactctcgag ccgaccccaa gaagaagcga aaggtgggat
     5881 ctggtggagg ctcctctaag ctgggcggct ctggcggctc tatggagcga gtgcgaatga
     5941 tcaacgtgca gcgactgctg gaggccgccg agttcctgga gcgacgagag cgagagtgcg
     6001 agcacggcta cgcctcttct ttcccctcta tgccctctcc ccgaggctag gctagcctca
     6061 tgtaattagt tatgtcacgc ttacattcac gccctccctc cacatccgct ctaaccgaaa
     6121 aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata gttatgttag
     6181 tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg
     6241 catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg aaggctttaa
     6301 tttgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6361 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6421 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6481 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     6541 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     6601 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     6661 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     6721 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     6781 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     6841 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     6901 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     6961 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7021 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7081 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7141 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7201 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7261 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7321 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7381 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7441 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7501 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7561 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     7621 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     7681 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     7741 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     7801 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     7861 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     7921 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     7981 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8041 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8101 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8161 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8221 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8281 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8341 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8401 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8461 cgaaaagtgc cacctgacgt ccccagttgc aaaagttgac acaactctag atctgcttcc
     8521 aaatatagaa tcataacaag ggttagggtg tgattatata atattggtct taattgatgt
     8581 gctagggctt taaaagttgg ttaaaataac gctctaatgc ctttttaata tattgtcttt
     8641 ttcaaaatct caaatcggac acttcttcgt gtatgagact ccattttttg gctccgtcac
     8701 gtgatatgta ttatcagcta tagtggtgta aacaaagttt tttactagct gtaatggcat
     8761 tttgtcggag tggtaaatcg ccttcttgtt gtgcgttcga gttctggact ctgcactggg
     8821 ctactttgaa aaatacctct aatgcgccga tggtttagtg gtaaaatcca tcgttgccat
     8881 cgatgggccc ccggttcgat tccgggtcgg cgcaggttga cctaggtttt agagctagaa
     8941 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     9001 ctttttttta cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     9061 tcacgaggcc cagatcctct agagtcgaag cggccgctat gtctgataaa aggatgtaac
     9121 ataggcaagc tgctcgtgag tgttgagtac gaaccttaga tccaaatcac ccgcacccac
     9181 ggatatactt gcttgaatat acagtagtat gcggccgctt cgacaccata tcatataaaa
     9241 ctaacaatgc attgaattaa tacgaagact acccgttgct atctccacac cgttatctcc
     9301 acggtccaaa ggctgctcaa tgtgctgcat acgtaacgtg gggtgcaacc ttgagcacat
     9361 agtactttcc gaaaaccggc gataattaag tgtgcactcc aacttttcac actgagcata
     9421 aaatgtggag aagaaatcac actaaaaagt caggtagact ggaaaaatgc gccatgaaat
     9481 aatatctctt gctacagtaa tgcccagcat cgaggggtat tgtgtcacca acactatagt
     9541 ggcagctgaa gcgctcgtga ttgtagtatg agtctttatt ggtgatggga agagtccact
     9601 caatattctc gttactgcca aaacaccacg gtaatcggcc agacaccatg gatgtagatc
     9661 accaagcctg tgaatgttat tcgagctaaa atgcacatgg ttggtgaaag gagtagttgc
     9721 tgtcgaattc cgtcgtcgcc tgagtcatca tttatttacc agttggccac aaacccttga
     9781 cgatctcgta tgtcccctcc gacatactcc cggccggctg gggtacgttc gatagcgcta
     9841 tcggcatcga caaggtttgg gtccctagcc gataccgcac tacctgagtc acaatcttcg
     9901 gaggtttagt cttccacata gcacgggcaa aagtgcgtat atatacaaga gcgtttgcca
     9961 gccacagatt ttcactccac acaccacatc acacatacaa ccacacacat ccacaatgga
    10021 acccgaaact aagaagacca agactgactc caagaagatt gttcttctcg gcggcgactt
    10081 ctgtggcccc gaggtgattg ccgaggccgt caaggtgctc aagtctgttg ctgaggcctc
    10141 cggcaccgag tttgtgtttg aggaccgact cattggagga gctgccattg agaaggaggg
    10201 cgagcccatc accgacgcta ctctcgacat ctgccgaaag gctgactcta ttatgctcgg
    10261 tgctgtcgga ggcgctgcca acaccgtatg gaccactccc gacggacgaa ccgacgtgcg
    10321 acccgagcag ggtctcctca agctgcgaaa ggacctgaac ctgtacgcca acctgcgacc
    10381 ctgccagctg ctgtcgccca agctcgccga tctctccccc atccgaaacg ttgagggcac
    10441 cgacttcatc attgtccgag agctcgtcgg aggtatctac tttggagagc gaaaggagga
    10501 tgacggatct ggcgtcgctt ccgacaccga gacctactcc gttcctgagg ttgagcgaat
    10561 tgcccgaatg gccgccttcc tggcccttca gcacaacccc cctcttcccg tgtggtctct
    10621 tgacaaggcc aacgtgctgg cctcctctcg actttggcga aagactgtca ctcgagtcct
    10681 caaggacgaa ttcccccagc tcgagctcaa ccaccagctg atcgactcgg ccgccatgat
    10741 cctcatcaag cagccctcca agatgaatgg tatcatcatc accaccaaca tgtttggcga
    10801 tatcatctcc gacgaggcct ccgtcatccc cggttctctg ggtctgctgc cctccgcctc
    10861 tctggcttct ctgcccgaca ccaacgaggc gttcggtctg tacgagccct gtcacggatc
    10921 tgcccccgat ctcggcaagc agaaggtcaa ccccattgcc accattctgt ctgccgccat
    10981 gatgctcaag ttctctctta acatgaagcc cgccggtgac gctgttgagg ctgccgtcaa
    11041 ggagtccgtc gaggctggta tcactaccgc cgatatcgga ggctcttcct ccacctccga
    11101 ggtcggagac tttgttgcca acaaggtcaa ggagctgctc aagaaggagt aagtcgtttc
    11161 tacgacgcat tgatggaagg agcaaactga cgcgcctgcg ggttggtcta ccggcagggt
    11221 ccgctagtgt ataagactct ataaaaaggg cccagccctg ctaatgaaat gatgatttat
    11281 aatttaccgg tgtagcaacc ttgactagaa gaagcagatt gggtgtgttt gtagtggagg
    11341 acagtggtac gttttggaaa cagtcttctt gaaagtgtct tgtctacagt atattcactc
    11401 ataacctcaa tagccaaggg tgtagtcggt ttattaaagg aagggagttg tggctgatgt
    11461 ggatagatat ctttaaagct ggcgactgca cccaacgagt gtggtggtag cttgttactg
    11521 tatattcggt aagatatatt ttgtggggtt tttagtggtg tttggtaggt tagtgtctgg
    11581 tatatgagtt gtaggcatga caatttggaa aggggtggac tttgggaata ttgtgggatt
    11641 tcaatacctt agtttgtaca gggtaattgt tacaaatgat acaaagaact gtatttcttt
    11701 tcatttgttt taattggttg tatatcaagt ccgttagacg agctcagtgc catggctttt
    11761 ggcactgtat ttcattttta gaggtacact acatccagtg aggtatggta aggttgaggg
    11821 cataatgaag gcaccttgta ctgacagtca cagacctctc accgagaatt ttatgagata
    11881 tactcgggtt cattttaggc tccgattcga ttcaaattat tactgtcgaa atcggttgag
    11941 catccgttga tttccgaaca gatctggccc tttcgtc
//
