LOCUS       sequence-192176-       10613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Esf1 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Esf1
FEATURES             Location/Qualifiers
     source          1..10613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(51..298)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     264..282
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        1795..2499
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2527..2967
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    3025..3049
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4940..4964)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      4994..5181
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5049..5073)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5205..5221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5205..5221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5218..5240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5229..5245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5253..5259,5260..5277,5278..5283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5298..5319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5436..5453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5607..6195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5687..5706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6366..7157,7158..7226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6989..7008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7227..7331)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7358..8700
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(8321..8368)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     8741..8759
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8797..8819)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8919..8938
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8959..9240
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             9241..9915
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(10018..10473)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10155..10176)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10367..10386
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(10599..10613,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(10613,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctttcgc gagctcgaga tcccgagctt gcaaattaaa
       61 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
      121 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      181 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      241 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgata
      301 tcgacaaagg aaaaggggcc tgtttactca caggcttttt tcaagtaggt aattaagtcg
      361 tttctgtctt tttccttctt caacccacca aaggccatct tggtactttt tttttttttt
      421 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      481 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      541 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      601 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      661 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      721 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      781 tttttttttt gatagaaata atacagaagt agatgttgaa ttagattaaa ctgaagatat
      841 ataatttatt ggaaaataca tagagttttt gttgatgcgc ttaagcgatc aattcaacaa
      901 caccaccagc agctctgatt ttttcttcag ccaacttgga gacgaatcta gctttgacga
      961 taactggaac atttgggatt ctacccttac ccaagatctt accgtaaccg gctgccaaag
     1021 tgtcaataac tggagcagtt tccttagaag cagatttcaa gtattggtct ctcttgtctt
     1081 ctgggatcaa tgtccacaat ttgtccaagt tcaagactgg cttccagaaa tgagcttgtt
     1141 gcttgtggaa gtatctcata ccaaccttac cgaaataacc tggatggtat ttatccatgt
     1201 taattctgtg gtgatgttga ccaccggcca tacctctacc accggggtgc tttctgtgct
     1261 taccgatacg acctttaccg gctgagacgt gacctctgtg ctttctagtc ttagtgaatc
     1321 tggaaggcat tcttgattag ttggatgatt gttctgggat ttaatgcaaa aaaatcacta
     1381 agaaggaaaa aaatcaacgg agaaagcaaa cgccatctta aatatacggg atacagatga
     1441 aaggtttgaa cctatctggg aaaatacgca ttaaacaagc gaaaaactgc gaggaaaatt
     1501 gtttgcgtct ctgcgggcta ttcacgcgcc agaggaaaaa taggaaaaat aacaggcatt
     1561 agaaaaataa ttttgatttt ggtaatgtgt gggtccctgg tgtacagatg ttacattggt
     1621 tacagtactc ttgtttttgc tgtgtttttc gatgaatctc caaaatggtt gttagcacat
     1681 ggaagagtca ccgatgctaa gttatctcta tgtaagctac gtggcgtgac ttttgatgaa
     1741 gccgcacaag agatacagga ttggcaactg caaatagaat ctggggatct agatatcctt
     1801 ttgttgtttc cgggtgtaca atatggactt cctcttttct ggcaaccaaa cccatacatc
     1861 gggattccta taataccttc gttggtctcc ctaacatgta ggtggcggag gggagatata
     1921 caatagaaca gataccagac aagacataat gggctaaaca agactacacc aattacactg
     1981 cctcattgat ggtggtacat aacgaactaa tactgtagcc ctagacttga tagccatcat
     2041 catatcgaag tttcactacc ctttttccat ttgccatcta ttgaagtaat aataggcgca
     2101 tgcaacttct tttctttttt tttcttttct ctctcccccg ttgttgtctc accatatccg
     2161 caatgacaaa aaaatgatgg aagacactaa aggaaaaaat taacgacaaa gacagcacca
     2221 acagatgtcg ttgttccaga gctgatgagg ggtatctcga agcacacgaa actttttcct
     2281 tccttcattc acgcacacta ctctctaatg agcaacggta tacggccttc cttccagtta
     2341 cttgaatttg aaataaaaaa aagtttgctg tcttgctatc aagtataaat agacctgcaa
     2401 ttattaatct tttgtttcct cgtcattgtt ctcgttccct ttcttccttg tttctttttc
     2461 tgcacaatat ttcaagctat accaagcata caatcaactc caagcttgaa gcaagcctcc
     2521 tgaaagatga agctactgtc ttctatcgaa caagcatgcg atatttgccg acttaaaaag
     2581 ctcaagtgct ccaaagaaaa accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt
     2641 cgctactctc ccaaaaccaa aaggtctccg ctgactaggg cacatctgac agaagtggaa
     2701 tcaaggctag aaagactgga acagctattt ctactgattt ttcctcgaga agaccttgac
     2761 atgattttga aaatggattc tttacaggat ataaaagcat tgttaacagg attatttgta
     2821 caagataatg tgaataaaga tgccgtcaca gatagattgg cttcagtgga gactgatatg
     2881 cctctaacat tgagacagca tagaataagt gcgacatcat catcggaaga gagtagtaac
     2941 aaaggtcaaa gacagttgac tgtatcgccg gaattgcaat acccagcttt gactcatatg
     3001 gccatggagg ccgaattagc tttcacaagt ttgtacaaaa aagcaggcta caaaatggct
     3061 ggagagaacc caaagaagga aggtgtagat gctaggtttg ccgggatcta cagcgatcct
     3121 aaatttaaga acacgaaaac caaagatcat aagatcaagt tagattctag gttcagtaag
     3181 aaggatctgg aggtccagca taaatctaag gttgacaagt atggtagaaa gatcaaaaat
     3241 gcgcagaata acagggaatt ggaagatttc gacaaatatt tcgaaaagga agcagaaaat
     3301 gatgaagatt cagaagttaa tgcgaaaaca gtagtggacc gtgcccgtgg tgaagttcct
     3361 gatgattatg ttagctcttc tgatgaattc acttcttctg attctgaatc gtccggagaa
     3421 agtgaagttg aaagcgaaga agaaaatgaa gtagaaattg aaaatgccaa accagaatct
     3481 ggtgacatct ctaagaattt ggccgtcgta aatttagatt gggaccatgt taaatctgag
     3541 gatttgatga ttaccttttc cagtttcgtt cctaaaggtg gtaaaattga aagagttgct
     3601 atttatccaa gtgaatttgg taaagaaaga atgcaacgcg aggaagttga aggacctcca
     3661 aaggaacttt tccaaaagaa aaataagaac aaaacctcca aaaagaagaa aactgatgac
     3721 tctgattctg atatggacat cggtattaag gatctttatg aagagggaga tgctgacaag
     3781 gacgtggatt ccagagcttt acgtcaatac cagttggaca gattaagata ttattacgca
     3841 atagtttatt gtagtgatac aaccacctcc aaagctatct atgataactg tgatggtacc
     3901 gaatatgagt caaccgctaa tatgttcgat ttaaggtatg ttcctgatgg tatgactttt
     3961 gatgatgatg taagagatga atgcagtata ttgcctaaga actataggcc acaccagttt
     4021 agcacagatg ctctacaaca ttcaagcgtc aaattgacct gggatgaaac gccagctgat
     4081 agggtcgaag ttgctaagag ggcctttacc caaaaggaaa ttgatgatat ggactttaag
     4141 gcttacttgg cgtcagatag tgatgaatcg gatgggcagg tcgatgaaga ggcgaaaaat
     4201 aaactaaagt cgttggttgg tgactttggg tttaattcca agaaagaaac ccctaacgat
     4261 gaagatgaag aagttgatat ggaaattaca tttactccag ccttggaagg aggaaacgag
     4321 aaatcgtcgg aagacaaaga agaaacgact atagagaaaa taagaagaaa agagaaggaa
     4381 cgtcgtaaag caagaaagca gaaagtaaag gaattgaaac agcagtcgga gaaggataag
     4441 aaaagcaagt tgaaatctgt taataaaaag catactaacg atgaagagga gatcgaaaag
     4501 aatgccaaat caaaggctga attggagctt ttaatggatg atgatgatga taccgaaact
     4561 cagggcacaa taaataacaa agcacatttc aacatgaacg aaatcttaag atcagaaaaa
     4621 gaaaaacaca agaaaggccg gtatcaaaag aaagagagaa ttgttgaaga cacctttaca
     4681 cctgatttag aagatcctag atttaaagaa gtcttcgaag atcacgattt tgctatcgac
     4741 ccgacccagc ctgaattcaa aggtacacaa gctatgagta aaatcctaaa agaacgtagt
     4801 aaacgtgtta agaataagaa aagaaaactt ggcggtagtg aaaacaatat gaccaataac
     4861 gcagatgata atgaagatat tggtaacttg gtaaacaagt tgaaaaagaa aagcaaatca
     4921 tccaagaagg taaaggttaa cccagctttc ttgtacaaag tggtgagtcg acctgcagcc
     4981 aagctaattc cgggcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     5041 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt
     5101 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     5161 tattgaccac acctctaccg gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     5221 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5281 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5341 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5941 ttatcgccac tggcagcagc cactagtaac aggattagca gagcgaggta tgtaggcggt
     6001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7321 ggggttccgc gcacatttcc ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt
     7381 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     7441 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     7501 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     7561 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     7621 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     7681 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     7741 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     7801 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     7861 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     7921 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     7981 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     8041 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     8101 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     8161 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     8221 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     8281 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     8341 agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa
     8401 tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat ctgcgtgttg
     8461 cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt aaatgcgtac
     8521 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc
     8581 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg
     8641 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc
     8701 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     8761 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     8821 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     8881 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     8941 gagagtgcac catagatcaa cgacattact atatatataa tataggaagc atttaataga
     9001 acagcatcgt aatatatgtg tactttgcag ttatgacgcc agatggcagt agtggaagat
     9061 attctttatt gaaaaatagc ttgtcacctt acgtacaatc ttgatccgga gcttttcttt
     9121 ttttgccgat taagaattaa ttcggtcgaa aaaagaaaag gagagggcca agagggaggg
     9181 cattggtgac tattgagcac gtgagtatac gtgattaagc acacaaaggc agcttggagt
     9241 atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag
     9301 agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt
     9361 gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta
     9421 aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa
     9481 cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat
     9541 ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc
     9601 gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt
     9661 attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt
     9721 tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga
     9781 ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta
     9841 agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa
     9901 aatgctaaga aataggttat tactgagtag tatttattta agtattgttt gtgcacttgc
     9961 cgatctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa
    10021 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    10081 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    10141 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    10201 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    10261 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    10321 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    10381 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    10441 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg
    10501 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
    10561 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gtt
//
