LOCUS       sequence-192181-        9752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Esf1 with Y2H activation domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pACT2-Esf1
FEATURES             Location/Qualifiers
     source          1..9752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(67..86)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     227..249
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        278..679
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             725..745
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             761..1102
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             1115..1141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1121..1147
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    1181..1205
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3096..3120)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3509..3696
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3564..3588)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(3717..3750)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4259..5353)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(5354..5759)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      6236..7578
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7199..7246)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7843..7947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7948..8016,8017..8808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8166..8185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8979..9567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9468..9487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9721..9738
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
       61 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
      121 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
      181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
      241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaacttct tttctttttt
      301 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
      361 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
      421 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
      481 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
      541 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
      601 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
      661 accaagcata caatcaactc caagctttgc aaagatggat aaagcggaat taattcccga
      721 gcctccaaaa aagaagagaa aggtcgaatt gggtaccgcc gccaatttta atcaaagtgg
      781 gaatattgct gatagctcat tgtccttcac tttcactaac agtagcaacg gtccgaacct
      841 cataacaact caaacaaatt ctcaagcgct ttcacaacca attgcctcct ctaacgttca
      901 tgataacttc atgaataatg aaatcacggc tagtaaaatt gatgatggta ataattcaaa
      961 accactgtca cctggttgga cggaccaaac tgcgtataac gcgtttggaa tcactacagg
     1021 gatgtttaat accactacaa tggatgatgt atataactat ctattcgatg atgaagatac
     1081 cccaccaaac ccaaaaaaag agatctctat ggcttaccca tacgatgttc cagattacgc
     1141 tagcttgggt ggtcatatgg ccatggaggc cccagctttc acaagtttgt acaaaaaagc
     1201 aggctacaaa atggctggag agaacccaaa gaaggaaggt gtagatgcta ggtttgccgg
     1261 gatctacagc gatcctaaat ttaagaacac gaaaaccaaa gatcataaga tcaagttaga
     1321 ttctaggttc agtaagaagg atctggaggt ccagcataaa tctaaggttg acaagtatgg
     1381 tagaaagatc aaaaatgcgc agaataacag ggaattggaa gatttcgaca aatatttcga
     1441 aaaggaagca gaaaatgatg aagattcaga agttaatgcg aaaacagtag tggaccgtgc
     1501 ccgtggtgaa gttcctgatg attatgttag ctcttctgat gaattcactt cttctgattc
     1561 tgaatcgtcc ggagaaagtg aagttgaaag cgaagaagaa aatgaagtag aaattgaaaa
     1621 tgccaaacca gaatctggtg acatctctaa gaatttggcc gtcgtaaatt tagattggga
     1681 ccatgttaaa tctgaggatt tgatgattac cttttccagt ttcgttccta aaggtggtaa
     1741 aattgaaaga gttgctattt atccaagtga atttggtaaa gaaagaatgc aacgcgagga
     1801 agttgaagga cctccaaagg aacttttcca aaagaaaaat aagaacaaaa cctccaaaaa
     1861 gaagaaaact gatgactctg attctgatat ggacatcggt attaaggatc tttatgaaga
     1921 gggagatgct gacaaggacg tggattccag agctttacgt caataccagt tggacagatt
     1981 aagatattat tacgcaatag tttattgtag tgatacaacc acctccaaag ctatctatga
     2041 taactgtgat ggtaccgaat atgagtcaac cgctaatatg ttcgatttaa ggtatgttcc
     2101 tgatggtatg acttttgatg atgatgtaag agatgaatgc agtatattgc ctaagaacta
     2161 taggccacac cagtttagca cagatgctct acaacattca agcgtcaaat tgacctggga
     2221 tgaaacgcca gctgataggg tcgaagttgc taagagggcc tttacccaaa aggaaattga
     2281 tgatatggac tttaaggctt acttggcgtc agatagtgat gaatcggatg ggcaggtcga
     2341 tgaagaggcg aaaaataaac taaagtcgtt ggttggtgac tttgggttta attccaagaa
     2401 agaaacccct aacgatgaag atgaagaagt tgatatggaa attacattta ctccagcctt
     2461 ggaaggagga aacgagaaat cgtcggaaga caaagaagaa acgactatag agaaaataag
     2521 aagaaaagag aaggaacgtc gtaaagcaag aaagcagaaa gtaaaggaat tgaaacagca
     2581 gtcggagaag gataagaaaa gcaagttgaa atctgttaat aaaaagcata ctaacgatga
     2641 agaggagatc gaaaagaatg ccaaatcaaa ggctgaattg gagcttttaa tggatgatga
     2701 tgatgatacc gaaactcagg gcacaataaa taacaaagca catttcaaca tgaacgaaat
     2761 cttaagatca gaaaaagaaa aacacaagaa aggccggtat caaaagaaag agagaattgt
     2821 tgaagacacc tttacacctg atttagaaga tcctagattt aaagaagtct tcgaagatca
     2881 cgattttgct atcgacccga cccagcctga attcaaaggt acacaagcta tgagtaaaat
     2941 cctaaaagaa cgtagtaaac gtgttaagaa taagaaaaga aaacttggcg gtagtgaaaa
     3001 caatatgacc aataacgcag atgataatga agatattggt aacttggtaa acaagttgaa
     3061 aaagaaaagc aaatcatcca agaaggtaaa ggttaaccca gctttcttgt acaaagtggt
     3121 gagtcgagag atctatgaat cgtagatact gaaaaacccc gcaagttcac ttcaactgtg
     3181 catcgtgcac catctcaatt tctttcattt atacatcgtt ttgccttctt ttatgtaact
     3241 atactcctct aagtttcaat cttggccatg taacctctga tctatagaat tttttaaatg
     3301 actagaatta atgcccatct tttttttgga cctaaattct tcatgaaaat atattacgag
     3361 ggcttattca gaagctttgg acttcttcgc cagaggtttg gtcaagtctc caatcaaggt
     3421 tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc aaatcgttgg
     3481 tagatacgtt gttgacactt ctaaataagc gaatttctta tgatttatga tttttattat
     3541 taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa
     3601 acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata
     3661 gcatgaggtc gctcttattg accacacctc taccggcatg ccggtcgagg gacctaataa
     3721 cttcgtatag catacattat acgaagttat attaagggtt attgaatatg atcggaattg
     3781 gtcgactacg tcgttaaggc cgtttctgac agagtaaaat tcttgaggga actttcacca
     3841 ttatgggaaa tggttcaaga aggtattgac ttaaactcca tcaaatggtc aggtcattga
     3901 gtgtttttta tttgttgtat tttttttttt ttagagaaaa tcctccaata tataaattag
     3961 gaatcatagt ttcatgattt tctgttacac ctaacttttt gtgtggtgcc ctcctccttg
     4021 tcaatattaa tgttaaagtg caattctttt tccttatcac gttgagccat tagtatcaat
     4081 ttgcttacct gtattccttt acatcctcct ttttctcctt cttgataaat gtatgtagat
     4141 tgcgtatata gtttcgtcta ccctatgaac atattccatt ttgtaatttc gtgtcgtttc
     4201 tattatgaat ttcatttata aagtttatgt acaaatatca taaaaaaaga gaatcttttt
     4261 aagcaaggat tttcttaact tcttcggcga cagcatcacc gacttcggtg gtactgttgg
     4321 aaccacctaa atcaccagtt ctgatacctg catccaaaac ctttttaact gcatcttcaa
     4381 tggccttacc ttcttcaggc aagttcaatg acaatttcaa catcattgca gcagacaaga
     4441 tagtggcgat agggttgacc ttattctttg gcaaatctgg agcagaaccg tggcatggtt
     4501 cgtacaaacc aaatgcggtg ttcttgtctg gcaaagaggc caaggacgca gatggcaaca
     4561 aacccaagga acctgggata acggaggctt catcggagat gatatcacca aacatgttgc
     4621 tggtgattat aataccattt aggtgggttg ggttcttaac taggatcatg gcggcagaat
     4681 caatcaattg atgttgaacc ttcaatgtag gaaattcgtt cttgatggtt tcctccacag
     4741 tttttctcca taatcttgaa gaggccaaaa cattagcttt atccaaggac caaataggca
     4801 atggtggctc atgttgtagg gccatgaaag cggccattct tgtgattctt tgcacttctg
     4861 gaacggtgta ttgttcacta tcccaagcga caccatcacc atcgtcttcc tttctcttac
     4921 caaagtaaat acctcccact aattctctga caacaacgaa gtcagtacct ttagcaaatt
     4981 gtggcttgat tggagataag tctaaaagag agtcggatgc aaagttacat ggtcttaagt
     5041 tggcgtacaa ttgaagttct ttacggattt ttagtaaacc ttgttcaggt ctaacactac
     5101 ctgtacccca tttaggacca cccacagcac ctaacaaaac ggcatcagcc ttcttggagg
     5161 cttccagcgc ctcatctgga agtggaacac ctgtagcatc gatagcagca ccaccaatta
     5221 aatgattttc gaaatcgaac ttgacattgg aacgaacatc agaaatagct ttaagaacct
     5281 taatggcttc ggctgtgatt tcttgaccaa cgtggtcacc tggcaaaacg acgatcttct
     5341 taggggcaga cattagaatg gtatatcctt gaaatatata tatatattgc tgaaatgtaa
     5401 aaggtaagaa aagttagaaa gtaagacgat tgctaaccac ctattggaaa aaacaatagg
     5461 tccttaaata atattgtcaa cttcaagtat tgtgatgcaa gcatttagtc atgaacgctt
     5521 ctctattcta tatgaaaagc cggttccggc gctctcacct ttcctttttc tcccaatttt
     5581 tcagttgaaa aaggtatatg cgtcaggcga cctctgaaat taacaaaaaa tttccagtca
     5641 tcgaatttga ttctgtgcga tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat
     5701 aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt cccacagttc
     5761 cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt
     5821 ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc
     5881 acttctacaa tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat
     5941 tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     6001 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     6061 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     6121 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     6181 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     6241 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     6301 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     6361 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgagagc gctaattttt
     6421 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     6481 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     6541 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     6601 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     6661 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     6721 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     6781 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     6841 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     6901 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     6961 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     7021 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     7081 ttgagcaatg tttgtggaag cggtattcgc aatattttag tagctcgtta cagtccggtg
     7141 cgtttttggt tttttgaaag tgcgtcttca gagcgctttt ggttttcaaa agcgctctga
     7201 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa
     7261 aacgagcgct tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc
     7321 acctatatct gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt
     7381 ttatgcttaa atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac
     7441 ctcctgtgat attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt
     7501 tagctgttct atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat
     7561 ttcctttgat attggatcat atgcatagta ccgagaaact agtgcgaagt agtgatcagg
     7621 tattgctgtt atctgatgag tatacgttgt cctggccacg gcagaagcac gcttatcgct
     7681 ccaatttccc acaacattag tcaactccgt taggcccttc attgaaagaa atgaggtcat
     7741 caaatgtctt ccaatgtgag attttgggcc attttttata gcaaagattg aataaggcgc
     7801 atttttcttc aaagctcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7861 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7921 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7981 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8041 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8101 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8161 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     8221 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8281 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8341 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8401 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8461 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     8521 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8581 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8641 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8701 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8761 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8821 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8881 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8941 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9001 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9061 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9121 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9181 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9241 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9301 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9361 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9421 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9481 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9541 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9601 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9661 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9721 agcgagtcag tgagcgagga agcggaagag cg
//
