LOCUS       sequence-192183-       11817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express yeast Kre33 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11817)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 11817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Kre33
FEATURES             Location/Qualifiers
     source          1..11817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(51..298)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     264..282
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        1715..2419
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2447..2887
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2945..2969
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6144..6168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      6198..6385
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6253..6277)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(6409..6425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6409..6425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6422..6444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6433..6449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6457..6463,6464..6481,6482..6487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6502..6523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6640..6657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6811..7399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6891..6910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7570..8361,8362..8430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8193..8212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8431..8535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8562..9904
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(9525..9572)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     9945..9963
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10001..10023)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10123..10142
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10163..10444
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             10445..11119
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(11222..11677)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11359..11380)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11571..11590
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctttcgc gagctcgaga tcccgagctt gcaaattaaa
       61 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
      121 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      181 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      241 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgata
      301 tcgacaaagg aaaaggggcc tgtttactca caggcttttt tcaagtaggt aattaagtcg
      361 tttctgtctt tttccttctt caacccacca aaggccatct tggtactttt tttttttttt
      421 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      481 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      541 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      601 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      661 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt gatagaaata
      721 atacagaagt agatgttgaa ttagattaaa ctgaagatat ataatttatt ggaaaataca
      781 tagagttttt gttgatgcgc ttaagcgatc aattcaacaa caccaccagc agctctgatt
      841 ttttcttcag ccaacttgga gacgaatcta gctttgacga taactggaac atttgggatt
      901 ctacccttac ccaagatctt accgtaaccg gctgccaaag tgtcaataac tggagcagtt
      961 tccttagaag cagatttcaa gtattggtct ctcttgtctt ctgggatcaa tgtccacaat
     1021 ttgtccaagt tcaagactgg cttccagaaa tgagcttgtt gcttgtggaa gtatctcata
     1081 ccaaccttac cgaaataacc tggatggtat ttatccatgt taattctgtg gtgatgttga
     1141 ccaccggcca tacctctacc accggggtgc tttctgtgct taccgatacg acctttaccg
     1201 gctgagacgt gacctctgtg ctttctagtc ttagtgaatc tggaaggcat tcttgattag
     1261 ttggatgatt gttctgggat ttaatgcaaa aaaatcacta agaaggaaaa aaatcaacgg
     1321 agaaagcaaa cgccatctta aatatacggg atacagatga aaggtttgaa cctatctggg
     1381 aaaatacgca ttaaacaagc gaaaaactgc gaggaaaatt gtttgcgtct ctgcgggcta
     1441 ttcacgcgcc agaggaaaaa taggaaaaat aacaggcatt agaaaaataa ttttgatttt
     1501 ggtaatgtgt gggtccctgg tgtacagatg ttacattggt tacagtactc ttgtttttgc
     1561 tgtgtttttc gatgaatctc caaaatggtt gttagcacat ggaagagtca ccgatgctaa
     1621 gttatctcta tgtaagctac gtggcgtgac ttttgatgaa gccgcacaag agatacagga
     1681 ttggcaactg caaatagaat ctggggatct agatatcctt ttgttgtttc cgggtgtaca
     1741 atatggactt cctcttttct ggcaaccaaa cccatacatc gggattccta taataccttc
     1801 gttggtctcc ctaacatgta ggtggcggag gggagatata caatagaaca gataccagac
     1861 aagacataat gggctaaaca agactacacc aattacactg cctcattgat ggtggtacat
     1921 aacgaactaa tactgtagcc ctagacttga tagccatcat catatcgaag tttcactacc
     1981 ctttttccat ttgccatcta ttgaagtaat aataggcgca tgcaacttct tttctttttt
     2041 tttcttttct ctctcccccg ttgttgtctc accatatccg caatgacaaa aaaatgatgg
     2101 aagacactaa aggaaaaaat taacgacaaa gacagcacca acagatgtcg ttgttccaga
     2161 gctgatgagg ggtatctcga agcacacgaa actttttcct tccttcattc acgcacacta
     2221 ctctctaatg agcaacggta tacggccttc cttccagtta cttgaatttg aaataaaaaa
     2281 aagtttgctg tcttgctatc aagtataaat agacctgcaa ttattaatct tttgtttcct
     2341 cgtcattgtt ctcgttccct ttcttccttg tttctttttc tgcacaatat ttcaagctat
     2401 accaagcata caatcaactc caagcttgaa gcaagcctcc tgaaagatga agctactgtc
     2461 ttctatcgaa caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa
     2521 accgaagtgc gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa
     2581 aaggtctccg ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga
     2641 acagctattt ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc
     2701 tttacaggat ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga
     2761 tgccgtcaca gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca
     2821 tagaataagt gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac
     2881 tgtatcgccg gaattgcaat acccagcttt gactcatatg gccatggagg ccgaattagc
     2941 tttcacaagt ttgtacaaaa aagcaggcta caaaatggcc aaaaaagcta tcgattcaag
     3001 aatcccgtca ctgatcagga atggtgttca aaccaaacaa aggtccatat ttgtcatcgt
     3061 tggggacagg gcacgtaacc agctcccaaa tttacattat ttgatgatga gtgctgacct
     3121 taagatgaac aagtccgtct tatgggctta taagaagaag cttctaggat tcacttcgca
     3181 cagaaaaaaa agagagaata aaattaagaa agaaattaaa agaggtacaa gggaagtgaa
     3241 tgaaatggac ccttttgaat cttttatctc taaccaaaat atcagatatg tatactacaa
     3301 agagagtgag aaaatattgg gtaataccta tggtatgtgt attcttcagg acttcgaggc
     3361 attaacccca aatcttttag caagaaccat tgaaactgta gaaggtggtg gtattgtggt
     3421 tattttattg aaatcaatgt catcgttaaa gcaactgtac accatgacta tggatgttca
     3481 cgcccgttat cgtactgagg ctcatggtga cgttgttgca agatttaacg aaagattcat
     3541 actttcttta ggttcaaatc caaactgttt agtcgttgat gatgaattaa atgttctacc
     3601 gctatctggt gccaaaaacg tgaaaccact acctcctaag gaagatgacg aattgcctcc
     3661 aaagcaatta gagttgcaag aattaaaaga atccttagaa gatgtccaac cagctggttc
     3721 attggtttct ctatccaaaa ctgtcaatca agcccacgct attctttctt tcattgatgc
     3781 catttcggaa aaaactttga atttcacagt tgcactaaca gctggaagag gtagaggtaa
     3841 gtctgctgct ttaggtattt ctattgccgc ggccgtttct cacgggtatt ctaatatttt
     3901 tgttacttct ccatctcccg aaaatttaaa gactctattt gagtttattt tcaaaggatt
     3961 cgatgcctta ggttaccaag aacacattga ttatgatatc attcaatcta cgaaccccga
     4021 cttcaacaaa gccattgtga gagttgatat taaaagagat cacagacaaa cgattcaata
     4081 tattgttcct caagaccatc aagttctggg ccaagctgaa ttagttgtca ttgatgaagc
     4141 tgcagctatt cctttgccaa tcgtcaagaa cttgctgggc ccatatctag tcttcatggc
     4201 atccactatc aatggttacg aaggtacggg tagatcttta tctctgaagt taattcagca
     4261 attgcgtaat caaaacaaca catccggtcg ggaaagtact caaaccgccg ttgtttcgag
     4321 agataataag gaaaaagact ctcacttaca ttctcaatcc cgtcaattac gtgaaatttc
     4381 cttggacgaa ccaatcagat atgctcctgg tgacccaatt gaaaaatggt taaacaagct
     4441 cttatgttta gatgttactt taattaaaaa cccaagattt gcaacaagag gaactcctca
     4501 cccatcccaa tgtaatctat ttgttgtcaa tagagatact ctattttctt atcatcctgt
     4561 ctcagaaaat ttcctggaaa aaatgatggc actgtacgtt tcatcccatt acaagaattc
     4621 tccaaacgac ttacaactga tgagtgacgc cccagcgcac aaactatttg ttcttttacc
     4681 accaattgat ccaaaggatg gcggcaggat ccctgaccct ctatgtgtca ttcaaattgc
     4741 attggaaggt gaaatcagta aggagagtgt cagaaattca ttatcgagag gccaaagagc
     4801 tggtggtgat ttaatcccat ggctgatctc tcaacaattc caagacgagg aattcgctag
     4861 tttgagtggt gctcgtatcg tcagaattgc cactaatcct gaatacgcat ccatgggcta
     4921 tggttctcgt gcaattgaat tactgagaga ctatttcgag ggtaaattca ctgacatgtc
     4981 tgaagatgtt cgtcctaagg attattctat aaagagggtg agcgataagg aactggccaa
     5041 aactaattta ttaaaggatg acgttaaatt aagagacgca aaaacgttgc cacctctact
     5101 attaaaactt tctgaacagc ctcctcatta tctacattat ttgggtgttt catatggttt
     5161 aacgcaatcg ttgcataaat tctggaagaa taacagcttt gttcctgttt atttacgtca
     5221 aaccgcaaac gatttaactg gtgaacatac ttgtgttatg ttgaacgttt tggaaggcag
     5281 agaatcaaac tggctcgttg aatttgccaa agatttccgt aaaagatttc tgtcgcttct
     5341 ttcttatgat tttcataagt ttactgccgt tcaagcctta agtgtcattg aaagtagtaa
     5401 gaaagcacaa gatttgtctg atgacgaaaa gcatgataat aaagaattga cgcggacgca
     5461 tctggatgac attttttctc catttgactt gaaaagattg gatagctact caaataattt
     5521 gctagattat cacgttatcg ttgatatgat acccatgctt gctcttttgt acttcggcga
     5581 taaaatggga gattcagtaa aactatcaag cgtccagagt gctatcctgt tggctatagg
     5641 gttacagcgt aaaaacatcg acactattgc caaggaattg aatttacctt ccaatcaaac
     5701 tattgctatg tttgcgaaga tcatgagaaa aatgtctcag tacttccgtc agctgttaag
     5761 ccaatcaatt gaagaaactc taccaaatat caaagatgat gctattgctg aaatggatgg
     5821 tgaagaaatc aaaaattaca acgccgcaga agcactggac caaatggaag aagatttaga
     5881 agaagccggt tctgaagccg ttcaggcgat gagggaaaaa caaaaagagc taatcaactc
     5941 cttgaactta gataaatacg ccataaatga taacagtgag gaatgggctg aatctcaaaa
     6001 atctttagaa atagctgcca aggccaaagg cgtcgtcagt ttaaaaactg gtaaaaagag
     6061 aacgactgaa aaggctgaag atatctatag acaagagatg aaagctatga aaaaaccaag
     6121 aaagtctaaa aaggctgcaa ataacccagc tttcttgtac aaagtggtga gtcgacctgc
     6181 agccaagcta attccgggcg aatttcttat gatttatgat ttttattatt aaataagtta
     6241 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     6301 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     6361 ctcttattga ccacacctct accggcatgc aagcttggcg taatcatggt catagctgtt
     6421 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6481 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6541 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6661 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7141 cgacttatcg ccactggcag cagccactag taacaggatt agcagagcga ggtatgtagg
     7201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7441 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7501 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8161 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8521 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gaacgaagca tctgtgcttc
     8581 attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     8641 gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg
     8701 cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     8761 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
     8821 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
     8881 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
     8941 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
     9001 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
     9061 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
     9121 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
     9181 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
     9241 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
     9301 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
     9361 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
     9421 tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt
     9481 tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt
     9541 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     9601 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
     9661 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
     9721 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     9781 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     9841 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     9901 gatcatacta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9961 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
    10021 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
    10081 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10141 tactgagagt gcaccataga tcaacgacat tactatatat ataatatagg aagcatttaa
    10201 tagaacagca tcgtaatata tgtgtacttt gcagttatga cgccagatgg cagtagtgga
    10261 agatattctt tattgaaaaa tagcttgtca ccttacgtac aatcttgatc cggagctttt
    10321 ctttttttgc cgattaagaa ttaattcggt cgaaaaaaga aaaggagagg gccaagaggg
    10381 agggcattgg tgactattga gcacgtgagt atacgtgatt aagcacacaa aggcagcttg
    10441 gagtatgtct gttattaatt tcacaggtag ttctggtcca ttggtgaaag tttgcggctt
    10501 gcagagcaca gaggccgcag aatgtgctct agattccgat gctgacttgc tgggtattat
    10561 atgtgtgccc aatagaaaga gaacaattga cccggttatt gcaaggaaaa tttcaagtct
    10621 tgtaaaagca tataaaaata gttcaggcac tccgaaatac ttggttggcg tgtttcgtaa
    10681 tcaacctaag gaggatgttt tggctctggt caatgattac ggcattgata tcgtccaact
    10741 gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag ttattaaaag
    10801 actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga aacctcattc
    10861 gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg attggaactc
    10921 gatttctgac tgggttggaa ggcaagagag ccccgaaagc ttacatttta tgttagctgg
    10981 tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta ttggtgttga
    11041 tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag caaatttcgt
    11101 caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt gtttgtgcac
    11161 ttgccgatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    11221 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
    11281 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
    11341 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
    11401 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
    11461 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
    11521 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
    11581 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
    11641 acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca
    11701 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    11761 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgtt
//
