LOCUS       sequence-192190-        4045 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains expanded CGG repeats (98 repeats with 2 AGG interruptions) 
            within the 5'UTR of FMR1. Internal control with synthetic primer 
            sequence for capillary electrophoresis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Hayward BE, Usdin K
  TITLE     Improved Assays for AGG Interruptions in Fragile X Premutation 
            Carriers.
  JOURNAL   J Mol Diagn. 2017 Nov;19(6):828-835. doi: 
            10.1016/j.jmoldx.2017.06.008. Epub 2017 Aug 14.
  PUBMED    28818679
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmoldx.2017.06.008"; journalName: "J Mol Diagn"; date: 
            "2017-11"; volume: "19"; issue: "6"; pages: "828-835"
COMMENT     Sequence Label: pGLACTE-PseudoHT51
FEATURES             Location/Qualifiers
     source          1..4045
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      140..188
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     polyA_signal    448..569
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(485..504)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     539..558
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(738..755)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1668..2459,2460..2528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2291..2310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2529..2633)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2660..3115
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2747..2766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2957..2978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    3346..3414
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    3610..3628
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3646..3664
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3682..3700
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3718..3736
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3754..3772
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3790..3808
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3826..3844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3889..3913
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggcctcgagc gcccgcagcc cacctctcgg gggcgggctc ccggcgctag cagggctgaa
       61 gagaagatgg aggagctggt ggtggaagta cattgcgcag gaggcatttc tcgcggctcc
      121 atggaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg
      181 gagattttca acatgaaaaa attattattt gatgatcaga tagcggcggg gaactgccag
      241 acatcaaata aaacaaaagg ctcagtcgga agactgggcc ttttgtttta tctgttgttt
      301 gtcggtgaac actctcccgg atccaccgga tctagataac tgatcataat cagccatacc
      361 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      421 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      481 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      541 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgttg cggccgcatc
      601 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
      661 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
      721 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2641 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3061 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat
     3121 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     3181 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
     3241 gccgcggtca ggacctttta tatcggcctc tatagtctca tctcttggtc tcggctgtgt
     3301 tcggtgggac gcttgcgatt tgctgatatg taatttcaag ttattctcga agccgcggtg
     3361 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatttcatt
     3421 ttggggttgc taatgcgcgt aggatacaga gagccagctc tcacgatagc cgtaagtttt
     3481 tgcgcctatg cccaagtatg acgcacttcg tttccgtggc caccaggggg cgccggtggc
     3541 cgccaggggg caccggtggc caccaggggg cgcctgaggc tttcgtcttc aagaattcct
     3601 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     3661 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     3721 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3781 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3841 gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
     3901 gaaccgtcag atcgcctgga gcaattccgg cgcgccgctc agctccgttt cggtttcact
     3961 tccggtggag ggccgcctct gagcgggcgg cgggccgacg gcgagcgcgg gcggcggcgg
     4021 tgacggaggc gccgctgcca ggggg
//
