LOCUS       sequence-192195-        4637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains normal CGG repeats (29 repeats with 2 AGG interruptions) 
            within the 5'UTR of FMR1. To generate capillary electrophoresis size
            standards..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Hayward BE, Usdin K
  TITLE     Improved Assays for AGG Interruptions in Fragile X Premutation 
            Carriers.
  JOURNAL   J Mol Diagn. 2017 Nov;19(6):828-835. doi: 
            10.1016/j.jmoldx.2017.06.008. Epub 2017 Aug 14.
  PUBMED    28818679
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmoldx.2017.06.008"; journalName: "J Mol Diagn"; date: 
            "2017-11"; volume: "19"; issue: "6"; pages: "828-835"
COMMENT     Sequence Label: pGLACTE-HT51-13
FEATURES             Location/Qualifiers
     source          1..4637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(133..157)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    202..220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    238..256
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    274..292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    310..328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    346..364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    382..400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    418..436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    632..700
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(931..1386)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1068..1089)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1280..1299
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1413..1517
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1518..1586,1587..2378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1736..1755)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2549..3137
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3038..3057
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3291..3308
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    3477..3598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3488..3507)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3542..3561
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    3722..3787
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(join(3788..4498,4499..4501,4502..4504))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3788..4498,4499..4501,4502..4504))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3830..3851)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4179..4198
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4438..4459
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccccctggca gcggcgcctc cgtcaccgcc gccgcccgcg ctcgccgtcg gcccgccgcc
       61 cgctcagagg cggccctcca ccggaagtga aaccgaaacg gagctgagcg gcgcgccgga
      121 attgctccag gcgatctgac ggttcactaa acgagctctg cttttatagg cgcccaccgt
      181 acacgcctaa agcttatacg ttctctatca ctgataggga gtaaactgga tatacgttct
      241 ctatcactga tagggagtaa actgtagata cgttctctat cactgatagg gagtaaactg
      301 gtcatacgtt ctctatcact gatagggagt aaactcctta tacgttctct atcactgata
      361 gggagtaaag tctgcatacg ttctctatca ctgataggga gtaaactctt catacgttct
      421 ctatcactga tagggagtaa actcgaggaa ttcttgaaga cgaaagcctc aggcgccccc
      481 tggtggccac cggtgccccc tggcggccac cggcgccccc tggtggccac ggaaacgaag
      541 tgcgtcatac ttgggcatag gcgcaaaaac ttacggctat cgtgagagct ggctctctgt
      601 atcctacgcg cattagcaac cccaaaatga aatgggtgag gtggagtacg cgcccgggga
      661 gcccaagggc acgccctggc acccgcaccg cggcttcgag aataacttga aattacatat
      721 cagcaaatcg caagcgtccc accgaacaca gccgagacca agagatgaga ctatagaggc
      781 cgatataaaa ggtcctgacc gcggccgctc caagtacctc ccgtacctta atattactta
      841 cttatcatgg tagcttgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      901 aacagttgcg cagcctgaat ggcgaatggc aaattgtaag cgttaatatt ttgttaaaat
      961 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     1021 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     1081 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     1141 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     1201 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     1261 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     1321 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     1381 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     1441 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     1501 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     1561 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     1621 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     1681 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1741 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1801 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1861 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1921 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1981 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2041 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2101 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2161 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     2221 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     2281 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     2341 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     2401 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     2461 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     2521 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     2581 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     2641 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2701 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2761 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2821 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2881 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2941 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3001 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3061 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3121 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3181 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3241 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3301 tgagcgagga agcggaagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     3361 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     3421 ttgaaggctc tcaagggcat cggtcgatgc ggccgcaacg cttacaattt acgcgttaag
     3481 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3541 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3601 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     3661 aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat ctagatccgg
     3721 tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct cgagatctga
     3781 gtccggactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     3841 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     3901 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     3961 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
     4021 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
     4081 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
     4141 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
     4201 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
     4261 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
     4321 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
     4381 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
     4441 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
     4501 ccatgggaac atcctttaca aatgccttgt aaaagcgcca ttggagcccc gcacttccac
     4561 caccagctcc tccatcttct cttcagccct gctagcgccg ggagcccgcc cccgagaggt
     4621 gggctgcggg cgctcga
//
