LOCUS       sequence-192197-        9243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle yeast Sen1 into Gateway destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 9243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pDONR221-Sen1
FEATURES             Location/Qualifiers
     source          1..9243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6832..6931)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6948..6967)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6949..6967)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6972..6988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6972..6988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7090..7109)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7101..7910
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7168..7187)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      8057..8645
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8546..8565
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8799..8816
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8975..9002)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(9094..9180)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(9229..9243,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9243,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagcagg ctacaaaatg aattccaaca atcctgataa taataatagc aacaacatta
      181 ataataataa taaggataag gatattgccc ccaatagcga tgttcagcta gctaccgtct
      241 ataccaaggc aaagtcttac attcctcaga ttgaacaagt ttatcaaggc acaaatccaa
      301 atattcaaga ggcaaaatta ttaggggagt tattacaagt tctagcagaa gttcccaagg
      361 gtacgcattt attttgcgac cccatacttg aaccaatatc aattttttcc ctaaccattt
      421 tctcattcaa tgaagaagct acggccacat ggctgaagaa tcattttaat ccaattctct
      481 ctgtttgtga taaatgcatt ctaaattttg cgagagggaa gtgcaaaatg ttacaacatt
      541 ttgccattca aagacatgtg ccgcatgaac atgtagccaa gtttaatgac atcgtatgcc
      601 aatggagggt ggaggctgtt tttcctattc ttagaaatat ttccgttaac gataatacgg
      661 gaattaacat tacaaatgaa attgaaactg caatgtacga gtgtctctgt aatccgcata
      721 tgttgaggct taacaagcaa cttaaggcta ctttcgaagc aatcttcaaa tttttctacg
      781 acacaaagca tcgtttgttg gacgttacga atccattaag cataaaaacc tttatatcag
      841 gtgtcatatt ttgctggtgt gaaggttcaa aagaggaaaa tgagtggtca cgagcttttc
      901 taaaggattt atactcaaga aatttccata taaatctttc taacctgaca ccggatatta
      961 ttgaggaagt ttatatccat attttattct tacagaatcc ggcaaactgg acagagattg
     1021 tggtatctca attctggtcg agattactac cggttttcaa tttatttgat aaagacgtct
     1081 tcatagagta ctttcaggtc ccaaaaaatg tggaatcttt aaaaaaaaca ttcaaatttc
     1141 cgttagagcc aatttttaaa atgtggtaca atcacttaag caaatcatat catgataagc
     1201 cactagactt tttgttaaga ggattaacaa tgttcttgaa taaatttggc tccgagtttt
     1261 ggtccaaaat tgaacccttc acattccata gcattttgga tataattttc aatagagatt
     1321 cattcccaat caaactaatc aaaattcaag acaatccaat tgttgagcat caaacagaag
     1381 tttactttca acttaccggt tctgtaaccg atttactttc ttggacctta ccattttatc
     1441 atgcactgtc accatccaaa agaattcaaa tggttagaaa ggtctcaatg gcgttcctgc
     1501 gtataatagc caattatcca tccctaaaat ccattcccaa agcttgttta atgaattcag
     1561 caactgcctt gttgagggct gtgttgacta ttaaggaaaa tgaaagggca atgctttata
     1621 agaatgatga atttgaaaca gtactgttga ctaaaacaga ttctagagct ttgctcaaca
     1681 atcctttgat ccaagacata ataattagat cagcttcgaa tcctaacgac ttttatcctg
     1741 ggctaggtgc tgcttctgca tcggtggcca catccacgat gatggtattg gcagaatgta
     1801 tcgattttga tatactgtta ttatgtcatc gaacgtttaa gctttattca ggtaaaccaa
     1861 ttagcgaaat tccaatttca accaacgtct tggaaaatgt tacaaataaa attgacctga
     1921 gatcttttca tgacggtcca ttgctggcca aacagctgct agtatcttta aaaaatatta
     1981 atggtctttt gatagtaccg tcaaatactg ccgtagctga ggcacacaac gcattgaatc
     2041 agaagttttt gctgctatca acgagattaa tggaaaaatt tgcggatatt ttgcccggtc
     2101 aactgtctaa aatccttgcg gatgaagatg catcgcaggg tttttggtcc tgcatattct
     2161 cctctgataa acacttgtat caagcagcta ctaatatttt atacaataca tttgacgttg
     2221 agggtagact ggaaggtatc ttagcgattc tgaatagtaa tttgaccgtt aacttgaaga
     2281 atataaacgt gatgctacaa agattaatca attgtgaatt ctatgagcct tgcccccgtg
     2341 ctgttcgtgt tttgatggac gtagtgagtg catttgtaga cccgatttct ggtgtgtttg
     2401 ctaatttcca aactttgaag agccaaaata ccgaaaagga atttttaaaa ttctgggaat
     2461 catgttggtt attcttggat acgatttata agtttacttt gaaatgggct tctaaatatg
     2521 actattctga actggaaaat ttcaccaaag atactttaga tctaagtcgc tctttggtgg
     2581 actcctttag agaattttca gatattcttc atgaccaaac gaaaaattta cttctcaacg
     2641 ttttagaaac ctttaagaat atgttgtatt ggctgagatt gagcgacgaa gtacttttgg
     2701 aatcttgtgt tagattaatt ataagcacgt ctgatttagc acatgaaaag catgtgaaag
     2761 tggatgattc cttggtagaa atgatggcca agtacgcctc gaaggcaaag agattttcaa
     2821 ataagttgac ggagcaacag gcgagtgaga ttctacaaaa ggctaaaata ttcaataaag
     2881 cactcacgga agaggtcgct acagaagctg aaaactacag aaaagaaaag gaattatcaa
     2941 gacttgggaa ggtgatcgac ttaacggatt ctgtgccggc gtcaccatcc ctctcgccct
     3001 ctctgtcttc aaccattgct agttcttctg ctgaatccag ggcagactat ttacaaagaa
     3061 aagctttatc ttcttcaata actgggagac caagagtagc tcagcctaaa ataacatctt
     3121 ttggtacgtt ccaatcatca gctaatgcta aactgcatcg tacaaaacct gttaagcctt
     3181 tatccaaaat ggaattagct aggatgcaat tattaaacaa cagagttgtt catccaccta
     3241 gtgcgccagc tttccataca aagagtaggg gtttgtctaa caaaaacgac gatagcagta
     3301 gtgaggagag tgacaacgat atagaaagcg ctcgggagct ttttgctatt gctaaggcaa
     3361 aaggcaaagg aatacaaact gtcgatatta atggtaaggt tgtcaagaga caaactgcgg
     3421 ctgaattggc taaacaggag ttagaacata tgagaaagag attaaatgtt gatatgaacc
     3481 ctctatatga aatcatattg caatgggatt acaccagaaa tagtgaatat cctgatgatg
     3541 agcctattgg taattattct gacgtaaagg atttcttcaa ctctcctgct gactaccaaa
     3601 aagtcatgaa gcctttacta cttctagaat cttggcaagg tctatgttct tcacgtgata
     3661 gagaggacta taaacctttc agtattattg taggaaatcg aactgctgtg tcagatttct
     3721 atgatgtgta tgcctctgtc gctaaacaag tcatacagga ttgtggtatt tctgaatctg
     3781 acttaattgt aatggcttat ttacccgatt ttcgaccaga caaaaggttg tccagtgatg
     3841 atttcaagaa agcacaacat acatgtttag caaaggtgag aacattgaaa aataccaaag
     3901 gtggtaatgt tgatgtcaca ttacgaattc atagaaatca ttctttcagt aaatttttga
     3961 cactaagatc agagatatat tgtgtgaagg ttatgcaaat gacgactatt gaaagagagt
     4021 attcaacact ggaagggctg gaatactatg acttagttgg ccaaattcta caggctaaac
     4081 catcgcctcc cgtaaatgtg gatgcggcag aaattgaaac agttaagaaa agctataagc
     4141 tcaatacgtc gcaggctgag gccatcgtta attcggtatc taaagaaggt ttttctttaa
     4201 ttcagggtcc acctggtacg ggtaaaacaa agactatact gggtattatt ggttactttt
     4261 tgtctacaaa gaatgcctca tcttcaaatg tcatcaaagt gccactagaa aagaactcat
     4321 cgaatactga acaactgtta aaaaagcaaa aaattctaat ctgtgccccc agtaatgccg
     4381 ctgtggacga aatctgcctc agactgaaga gtggagtgta tgacaagcag gggcatcaat
     4441 ttaaacctca attggtccgt gtaggtaggt cagacgttgt aaacgttgca attaaggacc
     4501 ttactttaga agaacttgtg gataaaagga taggtgaaag gaattacgaa atcagaacag
     4561 atccggaact agaacgtaaa tttaataatg ctgtgactaa aagaagagaa ttgagaggaa
     4621 aattagactc tgagagtggt aatccagaaa gtccaatgtc aaccgaagat atcagtaaat
     4681 tacaacttaa gattagggag ctaagtaaaa taatcaatga acttggccgt gatagggacg
     4741 aaatgcgtga gaagaattcc gtaaattata ggaataggga tttggataga aggaatgcac
     4801 aggcccatat cttggcggtt agtgacatca tatgttcgac tttatctggt tcagcccatg
     4861 atgtactcgc aacaatgggt attaaatttg atacggtcat tatagatgaa gcatgtcaat
     4921 gtaccgaact ttcttcaatt attcctctca ggtatggagg gaagcgttgt attatggttg
     4981 gtgatcctaa ccaactacca ccaactgttc tttcaggtgc agcaagcaat tttaaataca
     5041 atcagtcact gttcgttaga atggagaaaa atagctcccc atacttgctg gatgttcaat
     5101 accgtatgca tccttccatc agtaaattcc cctcttctga attttatcag ggacgactaa
     5161 aagacggccc tggtatggat atcttgaaca aaagaccctg gcaccagctt gagccattag
     5221 ctccctacaa attttttgac atcattagtg gtaggcaaga acaaaatgcg aagactatgt
     5281 cttatacaaa tatggaggaa atacgtgttg ccattgagtt agttgactac cttttcagaa
     5341 aattcgataa taaaattgat ttcacgggaa aaatcggtat aatttcacct tatagagagc
     5401 aaatgcaaaa aatgcgtaaa gagtttgccc gctattttgg aggcatgatc aacaaatcaa
     5461 tcgatttcaa tacaatcgac ggtttccaag gtcaagaaaa agaaatcatc ttgatatcgt
     5521 gtgtccgtgc cgatgatacg aaatctagtg tcggtttttt aaaggacttt cgtcgtatga
     5581 atgtggctct gactagagcc aagacaagta tttgggtttt gggacatcaa aggtctctgg
     5641 ctaaaagcaa gttatggaga gatttgattg aagatgcaaa agatagaagt tgtcttgcat
     5701 atgcttgctc gggtttcctg gatcccagaa ataatagagc tcaaagtatt ttaagaaagt
     5761 tcaatgtacc cgtaccatca gagcaagaag atgattataa gctacccatg gaatatatta
     5821 cgcaaggccc cgatgaggta aaatccaata aggacacaaa gaagagaaga gttgtcgatg
     5881 aaggtgaaga ggcagataaa gctgtaaaga aaaagaagaa agagaagaag aaggaaaaga
     5941 aaaagtccaa ggcagatgat aagaaaaaga ataacaagaa ggcagaatcg cccagcacct
     6001 catctggtac aaagaaaaag tcatccattt ttggtggaat gagtgtaccg agtgccgtag
     6061 ttcctaaaac ttttcctgat gtggacagca acaaaaaggc agcagcagtt gttggtaaaa
     6121 agaaaaataa caaacacgtt tgtttttcgg atgatgttag tttcatacca agaaatgacg
     6181 aacctgaaat taaagtgacc agaagtttat cctctgtatt gaaggaaaag cagttaggtt
     6241 tgaaggagac gagaacaatt tctccgcctg aaatcagtaa taatgaggat gatgatgacg
     6301 aagatgatta tacgccgtca atctccgatt cttcattaat gaaaagtgaa gcaaatggtc
     6361 gtaacaacag ggtggcttct cataaccaaa atttcagcgc tagtatatac gacgatccac
     6421 aagtttcgca agcaaagcag acgcaggtac ctgctgctat tacaaaacac agaagttcaa
     6481 atagtgtttt atccggtggt tcttcgagga tcctgactgc ttccgattac ggtgaaccca
     6541 atcaaaatgg acagaatgga gcaaatagga ccctctctca acatgtgggt aatgcgaatc
     6601 aatactcaac ggctcctgtg ggtacaggcg aactgcatga aactctgccc gcccatccgc
     6661 aagacagcta tcctgctgag gcagaagatc catatgattt aaacccccat ccgcaaccac
     6721 aatcttcagc attcaaagga cctggtagtg ggcccacagg cacacgcaac agttcgagac
     6781 ggaatgcttc atctagccca tttatcccaa aaaaaagaaa gcctagatca aacccagctt
     6841 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     6901 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     6961 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     7021 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     7081 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     7141 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     7201 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     7261 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     7321 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     7381 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     7441 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     7501 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     7561 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     7621 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     7681 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     7741 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     7801 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     7861 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     7921 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     7981 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     8041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     8101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     8161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     8221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     8281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     8341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     8401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     8461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     8521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8761 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8821 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     8881 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc
     8941 gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     9001 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     9061 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     9121 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     9181 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac
     9241 gtt
//
