LOCUS       sequence-192200-       15192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Brr2 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15192)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 15192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Brr2
FEATURES             Location/Qualifiers
     source          1..15192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(51..298)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     264..282
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        1769..2473
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2501..2941
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    2999..3023
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9519..9543)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9573..9760
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(9628..9652)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(9784..9800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9784..9800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9797..9819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9808..9824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9832..9838,9839..9856,9857..9862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9877..9898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10015..10032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10186..10774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10266..10285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10945..11736,11737..11805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11568..11587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11806..11910)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      11937..13279
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(12900..12947)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     13320..13338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13376..13398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     13498..13517
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        13538..13819
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             13820..14494
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(14597..15052)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14734..14755)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14946..14965
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(15178..15192,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(15192,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctttcgc gagctcgaga tcccgagctt gcaaattaaa
       61 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
      121 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      181 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      241 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgata
      301 tcgacaaagg aaaaggggcc tgtttactca caggcttttt tcaagtaggt aattaagtcg
      361 tttctgtctt tttccttctt caacccacca aaggccatct tggtactttt tttttttttt
      421 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      481 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      541 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      601 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      661 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      721 tttttttttt tttttttttt tttttttttt tttttttttt ttttgataga aataatacag
      781 aagtagatgt tgaattagat taaactgaag atatataatt tattggaaaa tacatagagt
      841 ttttgttgat gcgcttaagc gatcaattca acaacaccac cagcagctct gattttttct
      901 tcagccaact tggagacgaa tctagctttg acgataactg gaacatttgg gattctaccc
      961 ttacccaaga tcttaccgta accggctgcc aaagtgtcaa taactggagc agtttcctta
     1021 gaagcagatt tcaagtattg gtctctcttg tcttctggga tcaatgtcca caatttgtcc
     1081 aagttcaaga ctggcttcca gaaatgagct tgttgcttgt ggaagtatct cataccaacc
     1141 ttaccgaaat aacctggatg gtatttatcc atgttaattc tgtggtgatg ttgaccaccg
     1201 gccatacctc taccaccggg gtgctttctg tgcttaccga tacgaccttt accggctgag
     1261 acgtgacctc tgtgctttct agtcttagtg aatctggaag gcattcttga ttagttggat
     1321 gattgttctg ggatttaatg caaaaaaatc actaagaagg aaaaaaatca acggagaaag
     1381 caaacgccat cttaaatata cgggatacag atgaaaggtt tgaacctatc tgggaaaata
     1441 cgcattaaac aagcgaaaaa ctgcgaggaa aattgtttgc gtctctgcgg gctattcacg
     1501 cgccagagga aaaataggaa aaataacagg cattagaaaa ataattttga ttttggtaat
     1561 gtgtgggtcc ctggtgtaca gatgttacat tggttacagt actcttgttt ttgctgtgtt
     1621 tttcgatgaa tctccaaaat ggttgttagc acatggaaga gtcaccgatg ctaagttatc
     1681 tctatgtaag ctacgtggcg tgacttttga tgaagccgca caagagatac aggattggca
     1741 actgcaaata gaatctgggg atctagatat ccttttgttg tttccgggtg tacaatatgg
     1801 acttcctctt ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt
     1861 ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca
     1921 taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa
     1981 ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt
     2041 ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt
     2101 ttctctctcc cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca
     2161 ctaaaggaaa aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat
     2221 gaggggtatc tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct
     2281 aatgagcaac ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt
     2341 gctgtcttgc tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat
     2401 tgttctcgtt ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag
     2461 catacaatca actccaagct tgaagcaagc ctcctgaaag atgaagctac tgtcttctat
     2521 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa
     2581 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc
     2641 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct
     2701 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca
     2761 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt
     2821 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat
     2881 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc
     2941 gccggaattg caatacccag ctttgactca tatggccatg gaggccgaat tagctttcac
     3001 aagtttgtac aaaaaagcag gctacaaaat gactgagcat gaaacgaagg ataaggccaa
     3061 aaaaattaga gaaatttatc gttatgatga gatgtccaat aaggtgttga aagttgataa
     3121 acgctttatg aataccagcc aaaatcctca aagggatgcg gaaatatcac aaccaaaatc
     3181 catgagtggt agaattagtg ctaaggatat gggacagggc ctgtgcaata acattaataa
     3241 aggactaaaa gaaaatgatg tagccgttga aaaaactgga aagagcgcat cattgaaaaa
     3301 aattcagcag cataacacca ttttgaattc ttcctccgat ttcaggttac attattatcc
     3361 aaaggatccc tcaaatgtcg aaacttacga gcagattttg cagtgggtta cagaagtttt
     3421 aggcaatgat ataccccatg atcttattat tggaactgct gatatattca tcagacaatt
     3481 gaaagaaaat gaggaaaatg aggatggtaa tattgaagag cgaaaggaaa agatccagca
     3541 tgagctaggc atcaatatag actccctgaa gtttaacgaa ttagttaaac ttatgaaaaa
     3601 cattaccgat tatgaaacac atcctgacaa tagcaataaa caagcagtag caattcttgc
     3661 cgatgatgaa aaatcagatg aagaagaagt aacagaaatg tccaacaatg ctaatgttct
     3721 tgggggagaa atcaatgata atgaagatga tgatgaggaa tatgattaca atgacgttga
     3781 agtcaattct aaaaaaaaaa acaagagggc attgccaaac attgaaaacg atataattaa
     3841 actctccgat agtaaaacaa gtaatatcga gagcgttccg atatactcaa tcgatgaatt
     3901 ttttttgcag agaaaacttc ggtcagagct tggttacaag gatacctctg taatacaaga
     3961 cctgtcagaa aaaattttga atgatattga aacacttgag cataatccag tagcgttgga
     4021 gcagaagttg gttgatctgc ttaaattcga gaatatatca ctggctgaat ttattctcaa
     4081 gaatagatct acaatttttt ggggcatacg actggcgaag agcacagaga atgaaatacc
     4141 gaacttaata gagaaaatgg ttgcaaaagg tttgaatgat cttgtggaac agtataaatt
     4201 tagagaaact acacactcaa agcgtgaact cgattccggt gacgaccagc cacagtcttc
     4261 ggaagcaaaa agaacaaaat tcagtaatcc agcaataccg cccgtcatag atttggaaaa
     4321 gattaaattc gacgaaagtt ctaaattaat gaccgtgaca aaagtatccc taccggaagg
     4381 atcattcaaa agggtgaaac cacaatatga cgaaatacat attccagctc ctagcaaacc
     4441 tgttattgat tatgagttga aagaaattac ttctctacca gattggtgtc aggaggcgtt
     4501 tccttcatcg gaaacaacgt ccttaaaccc tatacaatca aaggtttttc acgcagcatt
     4561 tgaaggtgat tcaaacatgc taatatgtgc acccactggt tcaggtaaga cgaatatcgc
     4621 gttattgacg gtattgaaag ccttatccca tcattataac ccgaaaacta agaaattaaa
     4681 cctttctgca tttaaaattg tttatatagc acccttgaaa gccttagtac aggaacaagt
     4741 ccgagaattt caaaggaggt tggcttttct aggtataaaa gtcgctgaat tgactggtga
     4801 ttctaggctt agtagaaagc aaattgacga gacgcaggtt ttggtgtcta cgccagagaa
     4861 atgggacata actactagaa attcaaataa tttggccatc gtagaacttg ttcgtttgtt
     4921 gattatagat gaaatacatt tgttacatga tgacagggga ccagtattgg aaagcatcgt
     4981 ggctaggaca ttttgggctt ccaaatatgg ccaggaatac cccagaataa taggcctttc
     5041 tgccacctta ccaaattatg aggatgtcgg taggttttta agagttccga aggaaggatt
     5101 gttttatttt gactcctcat ttagaccgtg tcctctatct caacagtttt gtggcattaa
     5161 ggaacggaac tccttgaaaa agctgaaggc tatgaatgat gcttgttatg aaaaggtatt
     5221 ggaatccatc aatgaaggta atcaaataat cgtgtttgtt cattctcgaa aagaaacatc
     5281 acgaacagca acatggttga aaaataaatt tgctgaagag aacattactc ataaattaac
     5341 caaaaatgat gcaggatcga agcagatttt aaagacagaa gcagccaacg tactcgatcc
     5401 aagtctacga aagttgattg aaagcggtat agggacgcat cacgcagggc tgaccagaag
     5461 tgacagatcc ttatctgaag atttatttgc agacgggttg ttgcaagtat tggtatgcac
     5521 tgcgacgtta gcatggggtg ttaatctacc tgctcataca gtgattataa aaggaacaga
     5581 tgtctattca cctgaaaagg ggtcctggga acaactttct ccacaagatg tacttcaaat
     5641 gttaggtaga gcaggcagac cgagatatga cacatttggc gagggtatta taataacaga
     5701 ccaatcgaat gttcaatatt atctctcggt tttaaatcaa caattaccaa tagaatcgca
     5761 atttgtttca aaattagttg acaacttaaa tgcagaagtt gtcgcgggta acattaagtg
     5821 caggaatgat gctgtgaatt ggttggcgta tacttaccta tatgtcagaa tgttagcttc
     5881 ccctatgctt tacaaagtac ctgatatttc aagtgatggg caattgaaaa agtttagaga
     5941 aagcttagtc cattcggccc tatgcatttt aaaagaacaa gagttggtgt tatatgatgc
     6001 tgaaaatgat gtaattgagg cgaccgatct aggaaatata gcgtcgtcat tttacataaa
     6061 tcatgcttcc atggatgttt acaatagaga gttagatgaa catacaactc agattgatct
     6121 ttttagaata ttctcaatgt cggaagagtt taagtacgta tcggtgagat atgaagagaa
     6181 aagagaacta aaacaacttc tagagaaagc acccattcct atcagagaag atattgatga
     6241 tcctttagca aaagtgaatg tactactgca gtcttatttt tcccagctaa agttcgaagg
     6301 ttttgcactt aattctgata tagtgtttat tcaccaaaat gctggtaggt tattgcgtgc
     6361 tatgtttgaa atatgcttga agagaggatg gggacatccc acacggatgt tgttaaatct
     6421 ttgcaaatct gccacaacta aaatgtggcc aacaaactgt cctttaagac agtttaaaac
     6481 atgccctgtc gaagtgatta aaaggctgga agcctctaca gtaccgtggg gagattatct
     6541 acagctagaa acacctgccg aagttggtcg tgcaatcaga tctgaaaaat atggaaaaca
     6601 ggtttacgat ttattgaaaa gatttcccaa aatgtccgta acatgcaacg ctcagccaat
     6661 tacccgtagc gttatgaggt tcaacattga aataatagcc gactggatat gggatatgaa
     6721 tgtacacggt tcccttgaac cttttttgct catgctagaa gacactgatg gtgattcaat
     6781 actttattat gacgtactat tcatcactcc agatatagtt ggtcatgaat ttaccttatc
     6841 ctttacttat gagttaaagc agcacaatca aaataactta ccacctaatt tcttccttac
     6901 tttgatatca gaaaattggt ggcacagtga attcgagatt ccagtttcct tcaatggatt
     6961 taaacttccg aaaaaatttc ctcctcctac tccccttctg gagaatatta gtatttcaac
     7021 atcagagctt ggcaatgatg atttttccga agtatttgaa tttaaaactt tcaacaagat
     7081 tcagagccag gtcttcgagt ctctctacaa ttcgaatgac tcggtctttg taggttcagg
     7141 aaagggcaca ggtaaaacag ctatggctga attagcactg ttaaatcatt ggagacagaa
     7201 taaaggaaga gcggtttata tcaatccgtc tggggaaaaa attgattttt tgctatctga
     7261 ttggaataaa aggttctctc atcttgctgg tggtaagata ataaataagc taggaaatga
     7321 tccttcacta aatttaaaat tattagcaaa aagccatgtt ctcttggcta cccccgtgca
     7381 atttgagctt ttgtcacgtc gttggagaca aaggaagaac atccaaagtt tagaattaat
     7441 gatatatgat gacgctcatg agattagcca aggtgtttac ggtgctgtgt atgaaacgct
     7501 aatttctaga atgatattta tagcaaccca acttgaaaag aaaattcgat ttgtttgcct
     7561 ttcgaactgt ttggcaaatg ctcgtgattt tggtgaatgg gctggtatga cgaagtcaaa
     7621 catctacaac ttttctccaa gcgaaagaat tgagcctctt gaaattaata ttcagtcatt
     7681 taaagatgtt gagcacatat cattcaattt ttcgatgctt caaatggcat ttgaagcatc
     7741 tgctgctgca gcgggaaatc gtaattccag ttcggtgttc ttaccatcta gaaaggattg
     7801 tatggaagtt gcgtctgcat ttatgaagtt ttcaaaagca attgaatggg atatgttgaa
     7861 cgttgaagaa gagcaaattg taccatacat tgaaaagtta acagatggtc atctaagagc
     7921 acctttaaaa cacggtgttg gtattttgta taaggggatg gcatcaaatg atgaaagaat
     7981 tgtgaaaagg ttatacgaat atggtgcggt atcggtttta ctcatttcta aggattgttc
     8041 tgcattcgct tgtaaaactg atgaagtgat tattttagga accaaccttt atgatggtgc
     8101 agagcataaa tatatgccat ataccataaa tgaacttcta gaaatggtgg gactagctag
     8161 tggtaacgat tcaatggcag gcaaggtttt aattttgaca agtcacaaca tgaaggccta
     8221 ctacaaaaaa ttcttaattg agcctttgcc tactgagagt tacctacagt atattattca
     8281 tgatactctg aacaacgaga tagcaaattc tattatacaa agcaaacaag attgtgttga
     8341 ctggtttaca tattcgtatt tctatcgtcg tatccatgtg aatccaagct attatggcgt
     8401 aagggatacg tctccacatg gaatttcagt ttttttgagt aatttagttg aaacttgttt
     8461 gaatgactta gttgaatcat ccttcattga aattgacgat acagaagctg aagtaaccgc
     8521 agaagttaat ggaggagatg atgaagcaac tgaaatcatt tctaccttga gcaacgggct
     8581 aattgcttct cactatggcg tatcattttt tactattcag tcattcgttt cttctttatc
     8641 gaatacgtca acgctcaaaa acatgctgta tgttctttca acagcagtag aattcgaaag
     8701 tgttccactc agaaaaggag acagagcatt acttgtcaaa ttaagtaaaa gactaccatt
     8761 gagattccct gaacacacct cttctggttc ggtgagcttt aaagttttct tattgttaca
     8821 agcatatttt tcacgtcttg aattacctgt cgactttcaa aatgatttaa aagacattct
     8881 agaaaaggtt gttccactaa ttaacgtagt ggttgatatc ctttcagcaa acgggtattt
     8941 gaatgctact accgccatgg atttggcgca gatgttgatt cagggtgtat gggacgttga
     9001 taatccgttg agacagattc cacacttcaa caacaaaatt ttggaaaaat gtaaagaaat
     9061 taacgtcgag acggtctatg atataatggc ccttgaggat gaagagaggg atgaaatttt
     9121 aacgcttact gattcacagc ttgcgcaggt cgccgcgttt gttaacaatt accccaacgt
     9181 tgaacttact tattcgttga ataattctga ttcgttaatt tcaggtgtaa aacagaaaat
     9241 taccattcaa ttgacaagag atgttgaacc tgagaactta caagtaacat ctgaaaagta
     9301 tccgtttgat aagctagaaa gttggtggtt ggttttaggt gaagtatcta aaaaagagct
     9361 ttatgcgatc aagaaagtta ctttaaataa agagactcag caatatgaat tggaatttga
     9421 cactccgaca tctggtaaac ataacttaac aatttggtgt gtctgtgatt catatcttga
     9481 cgcagataaa gagttgtcct ttgaaataaa tgtgaaaaac ccagctttct tgtacaaagt
     9541 ggtgagtcga cctgcagcca agctaattcc gggcgaattt cttatgattt atgattttta
     9601 ttattaaata agttataaaa aaaataagtg tatacaaatt ttaaagtgac tcttaggttt
     9661 taaaacgaaa attcttattc ttgagtaact ctttcctgta ggtcaggttg ctttctcagg
     9721 tatagcatga ggtcgctctt attgaccaca cctctaccgg catgcaagct tggcgtaatc
     9781 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     9841 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     9901 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     9961 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    10021 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    10081 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    10141 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    10201 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    10261 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    10321 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    10381 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    10441 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    10501 caacccggta agacacgact tatcgccact ggcagcagcc actagtaaca ggattagcag
    10561 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    10621 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    10681 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    10741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    10801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    10861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    10921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    10981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    11041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    11101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    11161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    11221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    11281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    11341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    11401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    11461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    11521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    11581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    11641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    11701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    11761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    11821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    11881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgaacg
    11941 aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta atttttcaaa
    12001 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg ctattttacc
    12061 aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag cgctaatttt
    12121 tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga gagcgctatt
    12181 ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc gagagcgcta
    12241 tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct ataatgcagt
    12301 ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta ctttggtgtc
    12361 tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga ttactagcga
    12421 agctgcgggt gcattttttc aagataaagg catccccgat tatattctat accgatgtgg
    12481 attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt ggtcagaaaa
    12541 ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg tttacatttt
    12601 cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg tctaaagagt
    12661 aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt tcaaggagcg
    12721 aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca aagagatact
    12781 tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt acagtccggt
    12841 gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa aagcgctctg
    12901 aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
    12961 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
    13021 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
    13081 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
    13141 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
    13201 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
    13261 tttcctttga tattggatca tactaagaaa ccattattat catgacatta acctataaaa
    13321 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
    13381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    13441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
    13501 catcagagca gattgtactg agagtgcacc atagatcaac gacattacta tatatataat
    13561 ataggaagca tttaatagaa cagcatcgta atatatgtgt actttgcagt tatgacgcca
    13621 gatggcagta gtggaagata ttctttattg aaaaatagct tgtcacctta cgtacaatct
    13681 tgatccggag cttttctttt tttgccgatt aagaattaat tcggtcgaaa aaagaaaagg
    13741 agagggccaa gagggagggc attggtgact attgagcacg tgagtatacg tgattaagca
    13801 cacaaaggca gcttggagta tgtctgttat taatttcaca ggtagttctg gtccattggt
    13861 gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt gctctagatt ccgatgctga
    13921 cttgctgggt attatatgtg tgcccaatag aaagagaaca attgacccgg ttattgcaag
    13981 gaaaatttca agtcttgtaa aagcatataa aaatagttca ggcactccga aatacttggt
    14041 tggcgtgttt cgtaatcaac ctaaggagga tgttttggct ctggtcaatg attacggcat
    14101 tgatatcgtc caactgcatg gagatgagtc gtggcaagaa taccaagagt tcctcggttt
    14161 gccagttatt aaaagactcg tatttccaaa agactgcaac atactactca gtgcagcttc
    14221 acagaaacct cattcgttta ttcccttgtt tgattcagaa gcaggtggga caggtgaact
    14281 tttggattgg aactcgattt ctgactgggt tggaaggcaa gagagccccg aaagcttaca
    14341 ttttatgtta gctggtggac tgacgccaga aaatgttggt gatgcgctta gattaaatgg
    14401 cgttattggt gttgatgtaa gcggaggtgt ggagacaaat ggtgtaaaag actctaacaa
    14461 aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt actgagtagt atttatttaa
    14521 gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata ccgcacagat gcgtaaggag
    14581 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
    14641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
    14701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
    14761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
    14821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
    14881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
    14941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
    15001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
    15061 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    15121 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    15181 agtcacgacg tt
//
