LOCUS       sequence-192201-        9416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express yeast Mrt4 with Y2H binding domain fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Vincent NG, Charette JM, Baserga SJ
  TITLE     The SSU processome interactome in Saccharomyces cerevisiae reveals 
            potential new protein subcomplexes.
  JOURNAL   RNA. 2017 Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117.
  PUBMED    29054886
REFERENCE   2  (bases 1 to 9416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2017 
            Oct 20. pii: rna.062927.117. doi: 10.1261/rna.062927.117."
COMMENT     Sequence Label: pAS2-1-Mrt4
FEATURES             Location/Qualifiers
     source          1..9416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(51..298)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     264..282
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        1774..2478
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             2506..2946
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    3004..3028
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3743..3767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3797..3984
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3852..3876)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4008..4024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4008..4024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4021..4043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4032..4048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4056..4062,4063..4080,4081..4086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4101..4122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4239..4256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4410..4998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4490..4509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5169..5960,5961..6029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5792..5811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6030..6134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6161..7503
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(7124..7171)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     7544..7562
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7600..7622)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7722..7741
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7762..8043
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             8044..8718
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(8821..9276)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8958..8979)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9170..9189
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9402..9416,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9416,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgcc aagctttcgc gagctcgaga tcccgagctt gcaaattaaa
       61 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
      121 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      181 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      241 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgata
      301 tcgacaaagg aaaaggggcc tgtttactca caggcttttt tcaagtaggt aattaagtcg
      361 tttctgtctt tttccttctt caacccacca aaggccatct tggtactttt tttttttttt
      421 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      481 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      541 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      601 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      661 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      721 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttg atagaaataa
      781 tacagaagta gatgttgaat tagattaaac tgaagatata taatttattg gaaaatacat
      841 agagtttttg ttgatgcgct taagcgatca attcaacaac accaccagca gctctgattt
      901 tttcttcagc caacttggag acgaatctag ctttgacgat aactggaaca tttgggattc
      961 tacccttacc caagatctta ccgtaaccgg ctgccaaagt gtcaataact ggagcagttt
     1021 ccttagaagc agatttcaag tattggtctc tcttgtcttc tgggatcaat gtccacaatt
     1081 tgtccaagtt caagactggc ttccagaaat gagcttgttg cttgtggaag tatctcatac
     1141 caaccttacc gaaataacct ggatggtatt tatccatgtt aattctgtgg tgatgttgac
     1201 caccggccat acctctacca ccggggtgct ttctgtgctt accgatacga cctttaccgg
     1261 ctgagacgtg acctctgtgc tttctagtct tagtgaatct ggaaggcatt cttgattagt
     1321 tggatgattg ttctgggatt taatgcaaaa aaatcactaa gaaggaaaaa aatcaacgga
     1381 gaaagcaaac gccatcttaa atatacggga tacagatgaa aggtttgaac ctatctggga
     1441 aaatacgcat taaacaagcg aaaaactgcg aggaaaattg tttgcgtctc tgcgggctat
     1501 tcacgcgcca gaggaaaaat aggaaaaata acaggcatta gaaaaataat tttgattttg
     1561 gtaatgtgtg ggtccctggt gtacagatgt tacattggtt acagtactct tgtttttgct
     1621 gtgtttttcg atgaatctcc aaaatggttg ttagcacatg gaagagtcac cgatgctaag
     1681 ttatctctat gtaagctacg tggcgtgact tttgatgaag ccgcacaaga gatacaggat
     1741 tggcaactgc aaatagaatc tggggatcta gatatccttt tgttgtttcc gggtgtacaa
     1801 tatggacttc ctcttttctg gcaaccaaac ccatacatcg ggattcctat aataccttcg
     1861 ttggtctccc taacatgtag gtggcggagg ggagatatac aatagaacag ataccagaca
     1921 agacataatg ggctaaacaa gactacacca attacactgc ctcattgatg gtggtacata
     1981 acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt ttcactaccc
     2041 tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt ttcttttttt
     2101 ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa aaatgatgga
     2161 agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt tgttccagag
     2221 ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca cgcacactac
     2281 tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga aataaaaaaa
     2341 agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt ttgtttcctc
     2401 gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt tcaagctata
     2461 ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgaa gctactgtct
     2521 tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa
     2581 ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa
     2641 aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa
     2701 cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct
     2761 ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat
     2821 gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat
     2881 agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact
     2941 gtatcgccgg aattgcaata cccagctttg actcatatgg ccatggaggc cgaattagct
     3001 ttcacaagtt tgtacaaaaa agcaggctac aaaatgccaa gatcaaaacg ttccaagcta
     3061 gtcactttag cacaaaccga taaaaagggc agagaaaata aagaaagaat tttcgatgaa
     3121 gtgagggaag ccttagatac ttatagatac gtctgggtcc tacatcttga cgacgtaaga
     3181 actccagttt tacaagaaat cagaacatct tgggcaggct ctaagttaat tatgggtaag
     3241 aggaaagttt tacaaaaagc attgggagaa aagagggaag aagaatacaa agaaaatctg
     3301 tatcaattga gtaagctttg tagcggtgtc actggtttat tgttcactga tgaggatgtc
     3361 aacactgtca aggaatactt taagtcatac gttcgttcag actattcaag accgaacacg
     3421 aaagcaccac taacatttac aattcctgag ggcattgtct actcacgtgg tggtcaaatt
     3481 ccagctgagg aagatgttcc aatgattcat tctttagagc caactatgag aaacaaattt
     3541 gaaattccaa ctaaaatcaa agctggtaag atcaccattg acagtccata tttagtttgc
     3601 actgaaggag aaaaattaga tgttcgtcaa gctttaatac tgaaacaatt cggtatcgct
     3661 gcttctgaat tcaaagtcaa ggtgtcggcc tactatgaca acgatagctc cactgttgaa
     3721 agcactaaca tcaacatgga aaacccagct ttcttgtaca aagtggtgag tcgacctgca
     3781 gccaagctaa ttccgggcga atttcttatg atttatgatt tttattatta aataagttat
     3841 aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac gaaaattctt
     3901 attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc
     3961 tcttattgac cacacctcta ccggcatgca agcttggcgt aatcatggtc atagctgttt
     4021 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4081 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4141 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4741 gacttatcgc cactggcagc agccactagt aacaggatta gcagagcgag gtatgtaggc
     4801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5221 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5401 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6121 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca
     6181 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     6241 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     6301 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     6361 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     6421 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     6481 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     6541 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     6601 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     6661 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     6721 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     6781 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
     6841 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
     6901 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
     6961 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
     7021 ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt
     7081 gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
     7141 tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga gcgcttccga
     7201 aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta tatctgcgtg
     7261 ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg cttaaatgcg
     7321 tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct gtgatattat
     7381 cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct gttctatatg
     7441 ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct ttgatattgg
     7501 atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     7561 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7621 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     7681 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7741 actgagagtg caccatagat caacgacatt actatatata taatatagga agcatttaat
     7801 agaacagcat cgtaatatat gtgtactttg cagttatgac gccagatggc agtagtggaa
     7861 gatattcttt attgaaaaat agcttgtcac cttacgtaca atcttgatcc ggagcttttc
     7921 tttttttgcc gattaagaat taattcggtc gaaaaaagaa aaggagaggg ccaagaggga
     7981 gggcattggt gactattgag cacgtgagta tacgtgatta agcacacaaa ggcagcttgg
     8041 agtatgtctg ttattaattt cacaggtagt tctggtccat tggtgaaagt ttgcggcttg
     8101 cagagcacag aggccgcaga atgtgctcta gattccgatg ctgacttgct gggtattata
     8161 tgtgtgccca atagaaagag aacaattgac ccggttattg caaggaaaat ttcaagtctt
     8221 gtaaaagcat ataaaaatag ttcaggcact ccgaaatact tggttggcgt gtttcgtaat
     8281 caacctaagg aggatgtttt ggctctggtc aatgattacg gcattgatat cgtccaactg
     8341 catggagatg agtcgtggca agaataccaa gagttcctcg gtttgccagt tattaaaaga
     8401 ctcgtatttc caaaagactg caacatacta ctcagtgcag cttcacagaa acctcattcg
     8461 tttattccct tgtttgattc agaagcaggt gggacaggtg aacttttgga ttggaactcg
     8521 atttctgact gggttggaag gcaagagagc cccgaaagct tacattttat gttagctggt
     8581 ggactgacgc cagaaaatgt tggtgatgcg cttagattaa atggcgttat tggtgttgat
     8641 gtaagcggag gtgtggagac aaatggtgta aaagactcta acaaaatagc aaatttcgtc
     8701 aaaaatgcta agaaataggt tattactgag tagtatttat ttaagtattg tttgtgcact
     8761 tgccgatcta tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     8821 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     8881 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8941 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     9001 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     9061 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     9121 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     9181 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     9241 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggctgcgcaa
     9301 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     9361 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgtt
//
