LOCUS       sequence-192207-        9449 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9449)
  AUTHORS   Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K
  TITLE     DNA dynamics during early double-strand break processing revealed by
            non-intrusive imaging of living cells.
  JOURNAL   PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 
            10.1371/journal.pgen.1004187. eCollection 2014 Mar.
  PUBMED    24625580
REFERENCE   2  (bases 1 to 9449)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-03-13"; volume: "10(3):e1004187. doi"; pages: "
            10.1371/journal.pgen.1004187. eCollection 2014 Mar"
COMMENT     Sequence Label: pCM184-ParB2::eGFP
FEATURES             Location/Qualifiers
     source          1..9449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(28..414,415..1035))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     943..962
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(1120..1144)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1141..1344)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1170..1190)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1345..1724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      1836..2023
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1891..1915)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(2081..2099,2100..2122,2123..2141,2142..2164,2165..2183,
                     2184..2206,2207..2225,2226..2248,2249..2267,2268..2290,
                     2291..2309,2310..2332,2333..2351)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             join(3646..3648,3649..3651,3652..4362)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     terminator      4397..4644
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4413..4431)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4667..4683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4667..4683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4680..4702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4691..4707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4715..4721,4722..4739,4740..4745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4760..4781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4898..4915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5069..5657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5149..5168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5828..6619,6620..6688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6451..6470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6689..6793)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6861..6879
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6899..7000
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             7001..7675
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     misc_feature    7514..8676
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      7514..8351
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     primer_bind     9418..9440
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9432..9449
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9433..9449
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc
       61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc
      121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc
      181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc
      241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa
      301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag
      361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc
      421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      481 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      601 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      841 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      961 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
     1021 atctaatcta gacatatgaa ttaattcggg gccgcggagg ctggatcggt cccggtgtct
     1081 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
     1141 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     1201 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     1261 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     1321 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     1381 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     1441 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     1501 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     1561 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     1621 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     1681 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacatg gcggtaatgt
     1741 tggacatgag ccaatataaa tgtacatatt atgatatgga tacaacgtat gcaatgggcc
     1801 aagctcctcg agtaattcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     1861 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     1921 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     1981 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     2041 acagggtaat atagatcaat tcctcgatcg agtttaccac tccctatcag tgatagagaa
     2101 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2161 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2221 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2281 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2341 agtgatagag aaaagtgaaa gtcgagctcg gtaccctatg gcatgcatgt gctctgtatg
     2401 tatataaaac tcttgttttc ttcttttctc taaatattct ttccttatac attaggtcct
     2461 ttgtagcata aattactata cttctataga cacgcaaaca caaatacaca cactaaatta
     2521 ccggatcaat tcgggggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
     2581 atacccgttt ttttgggcta gcagggaatt cgaatggcaa aggacacatc taaggacaaa
     2641 aaacccacgg gcaacttgca cttagcagct ggcttgttaa ggggtttagc acaggaaaat
     2701 gcagctttgg aaaccaggct tcctgagcca accgcagcgc cggcagcggt cgttgaggca
     2761 actccagcca gtgcgccagc tgcggcgacc ccggcaggaa cggcagacct tggtgctcct
     2821 caaaaggtac ttgttaagga ttgtatacca aacccattca atccgagggt attttatagc
     2881 gaatccagtc tacatgaact agcactaact ttaaaaagag aaggtcaaat tgaaccaatc
     2941 aaggttactc gtttgccaga gtttccaggt aagttagtcg tcattgatgg acaaagaaga
     3001 ctgagagcca cttctatcaa tggggacgaa acaataaatg caacctttag aacagatcat
     3061 accccagaac aactttacac tatcgcatat agggctaatc atgatcatga aagacaaacc
     3121 atatttgatg acgctgttgc ttggaagaga ttactggatg aaaaagtctt ctccgatcag
     3181 aatactttgg ctgagaagat tggtaaagac aaagcttcca taagtaaaac tttatctttg
     3241 aatgcattac ctaatacact tttagaaaga atggcatctg caaatgatgt cgtaggtctg
     3301 caagctgcat attttctgaa gttgatttat gaaaggttgg gagagccgac agcggataga
     3361 ctattgactg cagtgattga tagaaaaaaa agtgttagag atctggaaaa ttttttaagg
     3421 gctcaaagcg atggtacgaa aaaggctggt agaaccagat atagtgttag acacgatttt
     3481 gctttggaaa gtagagctat cggtcaactt aagacttacc cagacggtag attagattta
     3541 caattaaaag gtgtagatgc cagtcaccag gaggcgttgg ccgataagtt aaagaccgtg
     3601 atcgatgcat acgtcgcaga tttagcaaca gctacaagta agcatatggt ttctaagggt
     3661 gaagagttat ttactggtgt tgttccaatc ttagttgaat tggacggcga tgtgaacggg
     3721 cacaagttct cagtatccgg tgaaggagag ggtgatgcga cctatggaaa attgaccctg
     3781 aaatttattt gtactaccgg taaacttcct gtcccttggc caacattagt caccacatta
     3841 acttacggcg tacaatgctt ctctagatac ccagaccaca tgaagcaaca tgattttttt
     3901 aagtcagcta tgccagaggg ttacgtgcag gagcgtacta tattctttaa agatgacggt
     3961 aactataaga ctagagcgga agttaaattt gagggcgaca cgctagttaa cagaatcgag
     4021 cttaaaggga ttgacttcaa ggaagatggc aacattttgg gacataagtt agagtacaat
     4081 tacaatagtc ataacgtata tataatggct gataaacaga agaatggtat taaagttaac
     4141 ttcaagatcc gtcacaacat cgaagatggg tctgtccagt tggctgatca ttaccagcag
     4201 aacactccaa ttggtgatgg ccctgtgtta cttcctgata atcactactt aagtacccag
     4261 tctgccttgt caaaagaccc gaatgagaaa agagatcaca tggtcctttt ggaatttgtc
     4321 actgcagctg gtattacatt gggtatggac gagctgtaca agtaagcggc cgctagggcc
     4381 ctgcaggagg gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
     4441 acatccgctc taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat
     4501 ttttttatag ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt
     4561 ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg
     4621 ggacgctcga aggctttaat ttgcggccaa gcttggcgta atcatggtca tagctgtttc
     4681 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     4741 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4801 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     4861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     5041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     5101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     5161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     5221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     5281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     5341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     5401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     5461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     5521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5701 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5761 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5821 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5881 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5941 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     6001 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     6061 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     6121 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     6181 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     6241 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     6301 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     6361 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     6421 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     6481 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6541 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6601 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6661 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6721 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6781 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     6841 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattaa
     6901 ttcggtcgaa aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac
     6961 gtgagtatac gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac
     7021 aggtagttct ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg
     7081 tgcactagat tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac
     7141 aattgacccg gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc
     7201 aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc
     7261 tctggtcaat gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga
     7321 ataccaagag ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa
     7381 catactactc agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga
     7441 agcaggtggg acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca
     7501 agagagcccc gagagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg
     7561 tgatgcgctt agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa
     7621 tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat
     7681 tactgagtag tatttattta agtattgttt gtgcacttgc ctgcaagcct tttgaaaagc
     7741 aagcataaaa gatctaaaca taaaatctgt aaaataacaa gatgtaaaga taatgctaaa
     7801 tcatttggct ttttgattga ttgtacagga aaatatacat cgcagggggt tgacttttac
     7861 catttcaccg caatggaatc aaacttgttg aagagaatgt tcacaggcgc atacgctaca
     7921 atgacccgat tcttgctagc cttttctcgg tcttgcaaac aaccgccggc agcttagtat
     7981 ataaatacac atgtacatac ctctctccgt atcctcgtaa tcattttctt gtatttatcg
     8041 tcttttcgct gtaaaaactt tatcacactt atctcaaata cacttattaa ccgcttttac
     8101 tattatcttc tacgctgaca gtaatatcaa acagtgacac atattaaaca cagtggtttc
     8161 tttgcataaa caccatcagc ctcaagtcgt caagtaaaga tttcgtgttc atgcagatag
     8221 ataacaatct atatgttgat aattagcgtt gcctcatcaa tgcgagatcc gtttaaccgg
     8281 accctagtgc acttacccca cgttcggtcc actgtgtgcc gaacatgctc cttcactatt
     8341 ttaacatgtg gaattaattc tcatgtttga cagcttatca tcgaactcta agaggtgata
     8401 cttatttact gtaaaactgt gacgataaaa ccggaaggaa gaataagaaa actcgaactg
     8461 atctataatg cctattttct gtaaagagtt taagctatga aagcctcggc attttggccg
     8521 ctcctaggta gtgctttttt tccaaggaca aaacagtttc tttttcttga gcaggtttta
     8581 tgtttcggta atcataaaca ataaataaat tatttcattt atgtttaaaa ataaaaaata
     8641 aaaaagtatt ttaaattttt aaaaaagttg attataagca tgtgaccttt tgcaagcaat
     8701 taaattttgc aatttgtgat ttaggcaaaa gttactattt ctggctcgtg taatatatgt
     8761 atgctaatgt gaacttttac aaagtcgata tggacttagt caaaagaaat tttcttaaaa
     8821 atatatagca ctagccaatt tagcacttct ttatgagata tattatagac tttattaagc
     8881 cagatttgtg tattatatgt atttacccgg cgaatcatgg acatacattc tgaaataggt
     8941 aatattctct atggtgagac agcatagata acctaggata caagttaaaa gctagtactg
     9001 ttttgcagta atttttttct tttttataag aatgttacca cctaaataag ttataaagtc
     9061 aatagttaag tttgatattt gattgtaaaa taccgtaata tatttgcatg atcaaaaggc
     9121 tcaatgttga ctagccagca tgtcaaccac tatattgatc accgatatta ggacttccac
     9181 accaactagt aatatgacaa taaattcaag atattcttca tgagaatggc ccagcgatta
     9241 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     9301 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9361 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9421 agtcacgacg ttgtaaaacg acggccagt
//
