LOCUS       sequence-19221-e         636 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf144
FEATURES             Location/Qualifiers
     source          1..636
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             393..434
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             444..461
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(484..501)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    490..601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catgcttgca ttttgttatt cgttgcccaa
       61 tgcgggtgat gtaataaagg gcagagtata cgagaaggat tatgctctat atatttatct
      121 ttttgactat cctcactttg aagctatctt ggcagagagt gttaagatgc atatggatag
      181 atatgttgaa tatagggata aactggtagg gaaaactgta aaagttaaag tgattagagt
      241 tgattataca aaaggatata tagatgtcaa ttacaaaagg atgtgtagac atcaataagc
      301 aattcccggg gatccgaata aagggtcaag acaattctgc agatatccag cacagtggcg
      361 gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac cctctcctcg
      421 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
      481 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
      541 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
      601 cgcattgtct gagtaggtgt cattctattc tggggg
//
