LOCUS       sequence-19223-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf146
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             531..572
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             582..599
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(622..639)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    628..739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agtacccttc accgaattcc agctgaccac catgactttg gtacagcacg tggtaactat
       61 taaatctact tattgggtca ttccatggga gctagcctct tacgataatc caaacttgtt
      121 tacagcaatg attctaatgt ctcctctagt taatgcagat gctgtttcaa aactgaatct
      181 gctagctgcc aaacttatgg gaaccatcac actaaatgcg ccagtcggaa agctatgtcc
      241 agaatcagta tctagagata tggataaagt ttataaatac caatacgacc cattaatcaa
      301 tcatgaaaaa attaaggctg gatttgctag tcaggtcttg aaggctacca acaaggttag
      361 aaaaataatt tccaagatta acaccccccg actctcatac tccagggaac aaacaataag
      421 attagcgatg ttttaggcaa ttcccgggga tccgaataaa gggtcaagac aattctgcag
      481 atatccagca cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta
      541 tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt
      601 gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
      661 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
      721 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctat
//
