LOCUS       sequence-19224-e         705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid was designed for screening purposes and may contain 
            discrepancies relative to the current canonical GenBank version of 
            the gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Tscharke DC, Karupiah G, Zhou J, Palmore T, Irvine KR, Haeryfar SM, 
            Williams S, Sidney J, Sette A, Bennink JR, Yewdell JW
  TITLE     Identification of poxvirus CD8+ T cell determinants to enable 
            rational design and characterization of smallpox vaccines.
  JOURNAL   J Exp Med. 2005 January 3; 201(1): 95–104.
  PUBMED    15623576
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp 
            Med."; date: "2005-?-3"; volume: " 201(1)"; pages: " 95–104"
COMMENT     Sequence Label: DCT.VV.Orf147
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             388..429
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             439..456
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(479..496)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    485..694
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 taccgagctc ggatccagta cccttcaccg aattccagct gaccaccatg agtgcaaact
       61 gtatgttcaa tctggacaat gattacatat attggaaacc tattacatat cctaaggcat
      121 tagtattcat aagtcatgga gctggtaaac attctggacg ttatgacgaa ctagctgaaa
      181 acatatcatc gttaggaatt ttagtattct cacatgatca tattggacat ggaagaagta
      241 atggtgaaaa aatgatgatt gatgactttg gtacagcacg tggtaactat taagcaattc
      301 ccggggatcc gaataaaggg tcaagacaat tctgcagata tccagcacag tggcggccgc
      361 tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct cctcggtctc
      421 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
      481 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      541 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
      601 tgtctgagta ggtgtcattc tattctgggg gggtgggggt ggggcaggac agcaaggggg
      661 aggattggga agacaatagc aggcatgctg gggatgcggt gggct
//
